The effects of Nigella sativa oil on kidney nephron structure NMRI mice treated with high dose of silver nanoparticles

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

SMA shariatzadeh ¹ , Afsaneh Nemati ²

1 - Department of Biology, Faculty of Science, Arak University, Arak, Iran
2 - Department of Biology, Faculty of Science, Arak University, Arak, Iran

Received: 2017-07-17 Accepted : 2017-09-17 Published : 2017-05-22

Keywords: Biochemical parameters, Mice, kidney, Silver, Stereology, Black oil seeds,

Abstract :

Today, with the increasing use of silver nanoparticles in consumer products and medical products, including serious concerns have been expressed about the potential risks of nano-silver particles, the goal of this study was to evaluate the protective effects of Nigella sativa oil (NSO) as a potent antioxidant on changes histological kidney tissue and blood biochemical parameters in rats treated with high dose of silver nanoparticles (AgNPs), respectively. 24 adult male rats (NMRI) with an average weight of 25 to 30 g were randomly assigned to 4 groups of 6 rats including the control group, AgNPs (mg / kg / day500), NSO (ml / kg / day 5) and AgNPs + NSO split and both were treated orally for 35 days. At the end of the treatment period, mice, anatomy, left kidney removed, fix, molding, cutting and tissue processing was carried out using -Zan Han Hayden were stained. Renal tissue was evaluated parameters stereologically. Serum samples were analyzed. ANOVA and Tukey test to evaluate the study data. The differences in the extent (05/0 P <) were considered significant.In this study, a significant increase in mean total volume of renal corpuscle, glomeruli, Taft, Bowman's capsule membrane and a significant reduction in the volume of the entire space of Bowman's capsule AgNPs group compared to the control group. Malvndy‌Ldyyd rate and serum urea AgNPs compared to the control group showed a significant increase (05 / 0P <) the parameters listed in Group AgNPs + NSO in the control group was normal.

References:

[1] Almansour M. Jarra Q . Battah A. Jarrar B, (2015) ."Morphometric Alterations Induced by the Toxicity of Variable Sizes of Silver Nanoparticles." Int. J. Morphol 33.2: 544-552

[2] Al-Sa'aidi J. A. A. Al-Khuzai A. L. D. AlZobaydi N. F. H, (2009)."Effect of alcoholic extract of Nigella sativa on fertility in male rats." Iraqi J Vet Sci 23.Suppl II: 123-128.

[3] Asharani P.V. Wu.YL. Gong Z. Valiyaveettil S, (2008). " Toxicity of silver nanoparticles in zebrafish models. Nanotechnology". 19(25): 5102-10.

[4] Bayrak O. Bavbek N. Karatas O. F. Bayrak R. Catal F.Cimentepe E. Akbas A. Yildirim E. Unal D. Akcay A, (2008) ."Nigella sativa protects against ischaemia/ reperfusion injury in rat kidneys." Nephrology Dialysis Transplantation 23.7: 2206-2212.

[5] Burits. M and Bucar F, (2000). "Antioxidant activity of Nigella sativa essential oil." Phytotherapy Research 14.5: 323-328.

[6] Buzea C, Pacheco I, Robbie K. (2007). "Nanomaterials and nanoparticles:Sources and toxicity. Biointerphases".; 2(4): 17-71.

[7] Cameron JS. Greger R, (1998) ."Renal function and testing of function." Oxford textbook of clinical nephrology 1: 39-69.

[8] Chen X, Schluesener HJ. (2008)" Nanosilver: a nanoproduct in medical application. Toxicol Lett; 176 (1): 1–12.

[9] Danladi J. Ahmed S. A. Akpulu S. P. Owolagba G. K. Iduh M. U, Mairiga A, (2013)"Protective effect of cool extraction
of Black Seed (Nigella Sativa) oil against CCl4-Induced oxidative damages in wistar rats testis." IOSR-J Pharm Bio Sci 5: 6874.

[10] Dezfoolian A.R. Panahi M. Feizi F, (2009). "Stereological evaluation of renal glomeruli in offspring of diabetic female rats.": 17-22. [11]

[11] Fisher-Wellman K . Bloomer R.J, (2009)."Acute exercise and oxidative stress: a 30 year history." Dynamic Medicine 8.1: 1.

[12] Forbes JM. Cooper ME. Oldfield MD. Thomas MC,"Role of advanced glycation end products in diabetic nephropathy. (2003)" Journal of the American Society of Nephrology 14.suppl 3: S254-S258.

[13] Gaillet S. Rouanet J-M, (2015)."Silver nanoparticles: their potential toxic effects after oral exposure and underlying mechanisms–a review." Food and Chemical Toxicology 77: 58-63.

[14] Ghlissi Z. Hamden Kh, Mongi S. Zouheir S. Mounir Z.K. El Feki A. Hakim A, (2012)."Effect of Nigella sativa seeds on reproductive system of male diabetic rats." African Journal of Pharmacy and Pharmacology 6.20: 1444-

[15] Gibbons B, Warner L. (2005) "The role of antimicrobial silver nanotechnology. Medical Device and Diagnostic Industry Magazine".; 27(5): 164-169.

[16] Gromadzka-Ostrowska J. Dziendzikowska K. Lankoff A. Dobrzyńska M. Instanes C.Brunborg G. Gajowik A. Radzikowska J. Wojewódzka M. Kruszewski M (2012). "Silver nanoparticles effects on epididymal sperm in rats." Toxicology letters 214.3: 251-258

[17] Hadjzadeh M-A-R. Mohammadian N. Rahmani Z. Behnam Rassouli F. (2008) "Effect of Thymoquinone on Ethylene Glycol-Induced Kidney Calculi in Rats". Urol J.;5:149-55.

[18] Heidari Z. Dezfoulian A.R. Barbarestani M. and Noori S.M.H, (2002)."A stereological analysis of renal glomeruli following chronic lead intoxication in rat during a continuous period of 8 weeks." Acta Medica Iranica 40.2: 73-78.

[19] Hemalatha P. Reddy AG. Rani MU. Anandkumar A.Shivakuma P, (2013)"Arsenic-induced histological alterations in various organs in rats." Int J Life Sci Biotechnol Pharm Res 2: 119-27. [20]

[20] Hoseini L. Roozbeh J. Sagheb M. Karbalay-Doust S. Noorafshan, A, (2009) ."Nandrolone decanoate increases the volume but not the length of the proximal and distal convoluted tubules of the mouse kidney." Micron 40.2: 226230.

[21] Howard Vand Reed M, (2004). "Unbiased stereology: three-dimensional measurement in microscopy." Garland Science,

[22] Iqbal M Athar M, (1998). "Attenuation of iron-nitrilotriacetate (Fe-NTA)mediated renal oxidative stress, toxicity and hyperproliferative response by the prophylactic treatment of rats with garlic oil." Food and chemical toxicology 36.6: 485-495

[23] Kim W. Y. Kim J. Park J. D. Ryu H. Y. & Yu I. J, (2009). "Histological study of gender differences in accumulation of silver nanoparticles in kidneys of Fischer 344 rats." Journal of Toxicology and Environmental Health, Part A 72.21-22: 1279-1284.

[24] Mandarim-de-Lacerda C. A, (2003). "Stereological tools in biomedical research." Anais da Academia brasileira de Ciências 75.4: 469-486.

[25] Mansour M. Tornhamre S, (2004). "Inhibition of 5-lipoxygenase and leukotriene C4 synthase in human blood cells by thymoquinone." Journal of Enzyme Inhibition and Medicinal Chemistry 19.5: 431-436

[26] Murmu S and Shrivastava V. K, (2011)."Protective Action of an Antioxidant (vitamin-C) Against Bisphenoltoxicity in Cirrhinus mrigala (Ham.)." Turkish Journal of Fisheries and Aquatic Sciences 11.1.

[27] Naghsh N.Mashayekh A. Khodadadi S , (2013)."Effects of silver nanoparticle on lactate dehydrogenase activity and histological changes of heart tissue in male wistar rats." Journal of Fasa University of Medical Sciences 2.4: 303-307.

[28] Nyengaard J. R. Flyvbjerg A. Rasch R, (1993) "The impact of renal growth, regression and regrowth in experimental diabetes mellitus on number and size of proximal and distal tubular cells in the rat kidney." Diabetologia 36.11: 11261131.

[29] Panacek A, Kvitek L, prucek R, Kolar M. et al . (2006). "Silver colloid nanoparticles: synthesis, characterization, and antibacterial activity". J Phys Chem; 110(33): 16248-16253

[30] Pazvant G. Sahin B. Kahvecioglu K.O. Gunes H. Ince, N.G. . BacinogluD, (2009). "The volume fraction method for the evaluation of kidney: A stereological study." Ankara Univ. Vet. Fak. Derg 56: 233-239.

[31] Rateb A. & Abdel-Hafez A. M, (2013). "Effect of Nigella sativa oil on paracetamol-induced renal cortical damage in rats: light and electron microscopic study." Egyptian Journal of Histology 36.1: 127-138.

[32] Sardari R. R.R. Rezaei Zarchi S. Talebi A. Nasri S, Imani S.Khoradmehr A. Razavi Sheshde S. A, (2012). "Toxicological effects of silver nanoparticles in rats." African Journal of Microbiology Research 6.27: 5587-5593.

[33] Seyedalipour B. Barimani N. Dehpour Jooybari A.A. Hosseini S.M. OshriehM, (2015). "Histopathological Evaluation of Kidney and Heart Tissues after Exposure to Copper Oxide Nanoparticles in Mus musculus." Journal of Babol University of Medical Sciences 17.7: 44-50

[34] Shah SV. Baliga R. Rajapurkar M. Fonseca VA, (2007). "Oxidants in chronic kidney disease." Journal of the American Society of Nephrology 18.1: 16-28.

[35] Sharieatzadeh SM. MalkyRad A. Hovaida R. Rahzani K. AghaJohary M. Fazli D, (2011). "The effect of Nigella sativa on oxidative stress." Journal of Shahrekord University of Medical Sciences 12.4: 2126.

[36] Sichani K. Naghsh N. Razmi N, (2012). "Effects of Alcoholic Extract of Peganumharmala L. on Malondialdehyde Concentration and Catalaseand Glutathione Peroxidase Activity in Mice Treated with Nanosilver Particles".J Mazand Univ Med Sci; 22(95): 10-17 (Persian).

[37] Sies. H, "Oxidative stress: oxidants and antioxidants. (1997). " Experimental physiology 82.2: 291-295

[38] Sohini S. Rana S. V. S, (2007). "Protective effect of ascorbic acid against oxidative stress induced by inorganic arsenic in liver and kidney of rat." Indian journal of experimental biology 45.4: 371.

[39] Sriram M.I. Kanth S.B.M. Kalishwaralal K. Gurunathan S, (2010). "Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model." Int J Nanomedicine 5.1: 753-762

[40] Stebounova LV. Adamcakova-Dodd A. Kim JS. Park H, (2011). "Nanosilver induces minimal lung toxicity or inflammation in a subacute murine inhalation model." Particle and fibre toxicology 8.1: 1. 12

[41] Tang J. Xi T, (2008). "Status of biological evaluation on silver nanoparticles." Sheng wu yi xue gong cheng xue za zhi= Journal of biomedical engineering= Shengwu yixue gongchengxue zazhi 25.4: 958-961.

[42] Tayarani-Najaran Z. Sadeghnia H. R. Asghari M. Mousavi S.H, (2009). "Neuroprotective effect of Nigella sativa hydro alcoholic extract on serum/glucose deprivation induced PC12 cells death." Physiology and Pharmacology 13.3: 263270.

[43] Völker C.Oetken M. Oehlmann J, (2013). "The biological effects and possible modes of action of nanosilver." Reviews of Environmental Contamination and Toxicology Volume 223. Springer New York, 81-106.

[44] Wang Z. Xia T. Liu S, (2015). "Mechanisms of nanosilver-induced toxicological effects: more attention should be paid to its sublethal effects." Nanoscale 7.17: 7470-7481.

[45] Yamagishi S.I. and Imaizumi T, (2005). ”Diabetic vascular complications: pathophysiology, biochemical basis and potential therapeutic strategy." Current pharmaceutical design 11.18: 2279-2299.