Study of Biochemical Parameters and Antioxidant Enzymes Activities on Oryza sativa under Salt Stress

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Ali Asghar  Bagheri ¹ (Department of Biology, Islamic Azad University, Roudehen Branch,)
Fariba  Khosravinejad ² (Department of Biology,Islamic Azad University Roudehen Branch,)

Received: 2016-09-24 Accepted : 2016-12-10 Published : 2016-11-21

Keywords: salinity stress, Antioxidant enzymes, Proline, Oryza sativa, Malondialdehid,

Abstract :

Abiotic stress including Salt stress is the major limiting factors of growth and crop production in arid and semiaridregions. Many of the Salinity affects are seen at the cellular levels. In order to understand the response of Oryza sativa to salt stress, some of antioxidant enzyme activities, Lipid peroxidation and Proline content were analyzed. Oryza sativa plant was treated by NaCl 0(control), 100, 200 and 300 mM for 14 days. For this purpose, a factorial design based on completely randomized design with three replications was used. The results showed that Growth parameters for example fresh and dry weight and height of shoot decreased under increasing NaCl. With increasing the concentration of NaCl, The activity of Catalase(CAT) and Ascorbat peroxidase(APX) were increased, but Superoxide dismutase (SOD) activity increased with increasing NaCl to 200 mM and then decreased at 300mM. Proline (Pro) and Malondialdehyde(MDA) contents respectively increased 2 and 3.6-fold at 300mM NaCl relative to the 0mM NaCl. Our results showed that by increasing the activity of CAT, SOD and APX associated with increased Proline had a positive effect on salt tolerance of Oryza sativa.

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