بررسی پارامترهای بیوشیمیایی و فعالیت آنزیم های آنتی اکسیدانی برنج تحت تاثیر تنش شوری

الموضوعات :

علی اصغر باقری ¹ (گروه زیست شناسی،دانشگاه آزاد اسلامی واحد رودهن،)
فریبا خسروی نژاد ² (گروه زیست شناسی، دانشگاه آزاد اسلامی واحد رودهن،)

تاريخ الإرسال : 23 السبت , ذو الحجة, 1437 تاريخ التأكيد : 11 السبت , ربيع الأول, 1438 تاريخ الإصدار : 21 الإثنين , صفر, 1438

الکلمات المفتاحية: تنش شوری, پرولین, Oryza sativa, آنزیم های آنتی اکسیدان, مالون دی آلدئید,

ملخص المقالة :

تنش های غیر زیستی از جمله شوری، مهم ترین عوامل محدود کننده رشد و عملکرد گیاهان زراعی در مناطق خشک و نیمه خشک هستند. بسیاری از اثرات تنش شوری در سطوح سلولی اعمال می گردد. به منظور درک پاسخ برنج به تنش شوری، فعالیت بعضی آنزیم های آنتی اکسیدان، پراکسیداسیون لیپید و محتوای پرولین مورد بررسی قرار گرفت. گیاه برنج به مدت 14 روز تحت تاثیر غلظت های 0، 100، 200و 300 میلی مولار کلرید سدیم(NaCl) قرار گرفت. بدین منظور، آزمایش گلخانه ای در قالب طرح بلوک های کامل تصادفی با 3 تکرار انجام گرفت. نتایج نشان داد که با افزایش کلرید سدیم پارامترهای رشد برای مثال وزن تر و خشک و اندازه گیاه کاهش نشان داد. فعالیت آنزیم های کاتالاز(CAT) و آسکوربات پراکسیداز(POD) با افزایش غلظت کلرید سدیم افزایش یافت اما فعالیت آنزیم سوپراکسید دیسموتاز(SOD) با افزایش غلظت کلرید سدیم تا غلظت 200 میلی مولار افزایش و سپس در غلظت 300 میلی مولار کاهش نشان داد. محتوای پرولین(Pro) و مالون دی آلدئید(MDA) در غلظت 300 میلی مولار به ترتیب به میزان 2 و 6/3 برابر نسبت به غلظت صفر میلی مولار کلرید سدیم افزایش نشان داد. نتایج ما نشان داد که افزایش فعالیت آنزیم های کاتالاز، سوپراکسید دیسموتاز و آسکوربات پراکسیداز همراه با افزایش میزان پرولین درتحمل برنج به تنش شوری تاثیر مثبتی داشته است.

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