Evaluation of the Foliar Spraying Effects of Chitosan Nanoparticles and Salicylic Acid on the Petal Senescence and Postharvest Quality of Cut Roses cv. "Samuraie"

Subject Areas : Journal of Ornamental Plants

Vida Jodi ¹ (PhD. Student in Department of Horticultural Science, University of Tabriz, Iran)
Mansour Matloobi ² (Department of Horticultural Sciences, Tabriz University Faculty of Agriculture, Tabriz, Iran)
Asghar Ebrahimzadeh ³ (Assistant Professor, Department of Horticultural Sciences, University of Maragheh, Iran)
Gholam Reza Mahdavinia ⁴ (Professor, Dep. of Chemistry, Faculty of Basic Sciences, University of Maragheh, Iran)

Received: 2024-01-29 Accepted : 2024-04-14 Published : 2024-04-24

Keywords: Cut rose, Longevity, Chitosan, SA, Spray treatment,

Abstract :

Extending the flower vase life is very important in the floriculture industry. In present study, the effectiveness of chitosan and salicylic acid nanoparticles in delaying of the senescence of cut roses was evaluated. Experiments were performed in a completely randomized design with 9 treatments in six repetitions, and the treatments were applied by petal spraying every three days. Chitosan nanoparticles with two concentrations (0.1 and 0.5 mg/L) and SA with two concentrations (0.1 and 0.3 mM) on the postharvest quality of cut roses were tested. Biochemical and enzymatic traits were measured during a period of 15 days (1, 4, 7, 11, 15 days after harvest), whereas, the vase life, RWU and electrolyte leakage were recorded till the end of flowers vase life. The maximum vase life (17.8 days) observed in flowers subjected to foliar spraying of 0.5 mg L^-1 of chitosan nanoparticles with 0.3 mM salicylic acid, while shortest flower longevity (13.5 days) obtained in untreated flowers. Also, the amount of relative solution uptake absorption of flowers and flavonoid content with significant increase were observed in 0.5 mg/L chitosan nanoparticles with 0.3 mM salicylic acid treatment. Treatments containing chitosan nanoparticles, especially the combined treatment of 0.5 mg/L of chitosan nanoparticles with 0.3 mM SA, reduced the amount of electrolyte leakage of the petals and increasing the vase life. Moreover, the lowest activity of antioxidant enzymes including GPX, PPO, POD and hydrogen peroxide were observed in flowers sprayed with 0.5 mgL^-1 chitosan nanoparticles + 0.3 mM SA. Among chitosan nanoparticle treatments, the longest vase life was related to 0.5 mgL^-1 (15 days), which increased flowers vase life by 11% compared to the control. Between SA treatments, the concentration of 0.3 mM was the most effective with a 6% increase in flowers vase life comparing untreated flowers. Finally, the combined treatments of chitosan nanoparticles and SA beside improving postharvest quality of cut roses, caused a 31% increase in flower longevity comparing untreated flowers.

References:

Ahmadi, Z., Saber, M., Akbari, A., Mahdavinia, G.R. 2018. Encapsulation of Satureja hortensis L. (Lamiaceae) in chitosan/TPP nanoparticles with enhanced acaricide activity against Tetranychus urticae Koch (Acari: Tetranychidae). Ecotoxicology and Environmental Safety, 161:111-119
Ahmad, I., Saleem, M. and Dole, J. M. 2016. Postharvest performance of cut ‘White Prosperity’ gladiolus spikes in response to nano-and other silver sources. Canadian Journal of Plant Science. 96(3): 511-516
Ahmed, KBM., Khan, MMA., Siddiqui, H. and Jahan, A. 2020. Chitosan and its oligosaccharides, a promising option for sustainable crop production-a review. Carbohydrate Polymers, 227:115331. https://doi.org/10.1016/j.carbpol.2019.115331
Alaey, M., Babalar, M., Naderi, R. and Kafi, M. 2011. Effect of pre-and postharvest salicylic acid treatment on physio-chemical attributes in relation to vase-life of rose cut flowers. Postharvest Biology and Technology 61(1): 91-94
Alexieva,V., Sergiev, I., Mapelli, S. and Karano, v. 2001. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant, Cell & Environment, 24(12): 1337-1344
Alimoradi, M., Poor, M. J. and Golparvar, A. 2013. Improving the keeping quality and vase life of cut Astroemeria flowers by post-harvest nano silver treatment. International Journal of Agriculture and Crop Science, 6(11): 632-635
Armitage, A.M. and Laushman, J.M. 2003. Spatiality cut flowers. The production of annuals, perennials, bulbs, and woody plants for fresh and dried cut flowers, Timber Press, Portland Cambridge. 392
Arora, A. and Singh, V. P. 2004. Cysteine protease gene expression and proteolytic activity during floral development and senescence in ethylene-insensitive Gladiolus grandiflora. Journal of Plant Biochemistry and Biotechnology, 13 (2): 123-126. https://doi.org/10.1007/BF03263206
Bai, J. G., Xu, P. L., Zong, C. S. and Wang, C. Y. 2009. Effects of exogenous calcium on some postharvest characteristics of cut gladiolus. Agricultural Sciences in China, 8, 293-303
Bañuelos-Hernández, K.P., García-Nava, J.R. Leyva-Ovalle, O.R. Peña-Valdivia, C.B. Trejo, C. and Ybarra-Moncada, M.C. 2017. Chitosan coating effect on vase life of flowering stems of Heliconia bihai L. cv. Halloween. Postharvest Biology and Technology, 132:179-187
Bayat, H. and Aminifard, M. H. 2017. Salicylic acid treatment extends the vase life of five commercial cut flowers. Electronic Journal of Biology, 13(1): 67-72
Chalabi, S. K. and Nasreen Khalil A. 2013. Ornamental plants in Iraq. College of Agriculture, University of Baghdad, Ministry of Higher Education and Scientific Research, Iraq, p. 415
Chang, C., Yang, M., Wen, H and Chern, J. 2002. Estimation of total flavonoid content in Propolis by two complementary colorimetric methods. Journal of Food Drug Analysis, 10: 178 - 182
Choudhary, K., Chaudhary,N., Agrawal, S.B and Agrawal, M. 2017. Reactive Oxygen Species: Generation, Damage, and Quenching in Plants During Stress. Book Editor(s):Vijay Pratap Singh, Samiksha Singh, Durgesh Kumar Tripathi, Sheo Mohan Prasad, Devendra Kumar Chauhan.
Doi, M., Hu, Y. and Imanishi, H. 2000. Water relations of cut roses as influenced by vapor pressure deficits and temperatures. Journal of Japanese Society for Horticultural Science, 69: 584–589. https://doi.org/10.2503/jjshs.69.584
Ezhilmathi, K., Singh, V., Aroa, A and Sairam, R. 2007. Effect of 5-Sulfosalicylic acid on antioxidant activity in relation to vase life of gladiolus cut flowers. Journal of plant Growth Regulation, 51: 99-108
Gozukirmizi, N., Yilmaz, S., Marakli, S and Aslihan Temel, A. 2015. Retrotransposon-based molecular markers; Tools for variation analysis in plants. Applications of Molecular Markers in Plant Genome Analysis and Breeding, 2015: 19-45 ISBN: 978-81-308-0560-3 Editor: Ksenija Taški-Ajduković
Hassan, FAS., Mazrou, R., Gaber, A., Hassan, MM. 2020. Moringa extract preserved the vase life of cut roses through maintaining water relations and enhancing antioxidant machinery. Postharvest Biology and Technology, 164:111156. https://doi. org/10.1016/j.postharvbio.2020.111156
Hatamzadeh, A., Hatami, M and Ghasemnezhad, M. 2012. Efficiency of salicylic acid delay petal senescence and extended quality of cut spikes of Gladiolus grandiflora cv ‘wing’s sensation’. African Journal of Agricultural Research 7: 540-545
He, Y., Qian, L., Liu, X., Hu, R., Huang, M., Liu, Y., Chen, G., Losic, D and Zhu, H. 2018. Graphene oxide as an antimicrobial agent can extend the vase life of cut flowers. Nano Research 11(11), 6010–6022. https:// doi.org/10.1007/s12274-018-2115-8
Hojati, M., Mohammad Modarres-Sanavy, S. A., Karimi, M and Ghanati, F. 2011. Responses of growth and antioxidant systems in Carthamus tinctorius L. under water deficit stress. Acta Physiologiae Plantarum, 33:105–112
Hong-juan, J and Huan-qing, L. 2015. Chitooligosaccharide prolongs vase life of cut roses by decreasing reactive oxygen species. Horticultural Science and Technology, 33(3):383-389
In, BC., Ha, ST., Lee, YS and Lim, JH. 2017. Relationships between the longevity, water relations, ethylene sensitivity, and gene expression of cut roses. Postharvest Biology and Technology, 131:74–83. https:// doi.org/10.1016/j.postharvbio.2017.05.003
In, BC., Inamoto, K and Park, S. 2016. Using thermography to estimate leaf transpiration rates in cut roses for the development of vase life prediction models. Horticulture, Environment, and Biotechnology, 57:53–60. https://doi.org/10.1007/s13580-016-0117-6.
Ioannou, A., Gohari, G., Papaphilippou, P., Panahirad, S., Akbari, A., Dadpour, M. R., and Fotopoulos, V. 2020. Advanced nanomaterials in agriculture under a changing climate: The way to The future? Environmental and Experimental Botany, 104048
Jiang, Y., Khan, MA., Wang, Z., Liu, J., Xue, J and Gao J. 2015. Cu/ZnSOD involved in tolerance to dehydration in cut rose (Rosa hybrida). Postharvest Biology and Technology, 100:187–95
Jing, H.J. and Li, H.Q. 2015. Chitooligosaccaride prolongs vase life of cut roses by decreasing reactive oxygen species. Korean Jorurnal of Horticultural Science and Technology 33(3): 383-389
Jones, R and McConchie, R. 1995. Characteristics of petal senescence in a non-climacteric cut flower. In VI International Symposium on Postharvest Physiology of Ornamental Plants vol. 405, 216–223.
Maehly, A.C and Chance, B. 1995. Assay of catalases and peroxidases. Methods in Enzymology, 2: 764–817.
Mehdikhah, M., Onsinejad, R., Nabi Ilkaee.M and Kaviani, B. 2016. Effect of Salicylic Acid, Citric Acid and Ascorbic Acid on Postharvest Quality and Vase Life of Gerbera (Gerbera jamesonii) Cut Flowers. Journal of Ornamental jPlants, 6(3): 181-191
Mencarelli, F., Agostini, R., Botondi, R. and Massantini, R. 1995. Ethylene production, ACC content, PAL and POD activities in excised sections of straight and bent gerbera scapes. Journal of Horticultural Science. 70: 409- 416
Michalak, A. 2006. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish J. of Environ. Stud. 15(4): 523-530
Mohammadi Kabari, S. F and Jadid Soleimandarabi, M. 2019. Improving Alstroemeria vase life by plant extracts and 8-Hydroxyquinoline sulfate. Journal of Ornamental Plants. 9(1): 1-11
Mohammadi, Z and mortazavi, N. 2014. The Effect of Sucrose and Salicylic acid on Longevity and Postharvest Quality in Alstroemeria (cv. Stratus) Cut Flower. Journal of Horticultural Science. 28 (4): 505-516
Moulaei,M., Homayoun Farahmand, H and Nasibi,F. 2021. Vase life and antioxidant status of two carnations (Dianthus caryophyllus L.) cultivars affected by gamma aminobutyric acid (GABA) treatments. Journal of horticulture and postharvest research. 4(4): 497-508
Nguyen, T.B.T.; Ketsa, S.; van Doorn, W.G. 2003. Relationship between browning and the activities of polyphenol oxidase and phenylalanine ammonia lyase in banana peel during low temperature storage. Postharvest Biology and Technology, 30: 187-93
Okigbo, R. N and Ogbonnaya, U. O. 2006. Antifungal effects of two tropical plant leaf extracts (Ocimum gratissimum and Aframomum melegueta) on postharvest yam (Dioscorea spp.) rot. African Journal of Biotechnology, 5(9):727-731
Oraee T, Asgharzadeh A, Kiani M, Oraee A. 2011. The role of preservative compounds on number of bacteria on the end of stems and vase solution of cut Gerbera. Journal of Ornamental and Horticultural Plants, 1: 161-166
Parween, A and Gupta, P. 2022. Study on postharvest life of cut gerbera flowers as affected by different organic and inorganic preservative solutions. The Pharma Innovation Journal, 11(9): 1600-1603
Pompodakis, N.E., Joyce, D.C., Terry, L.A and Lydakis, D.E. 2004. Effects of vase solution pH and ABA on the longevity of cut ‘Baccara’ roses. The Journal of Horticultural Science and Biotechnology, 79: 828-832
Reddy, K., Zullo, J., Bertolino, E and Singh, E. 2008. Transcriptional repression mediated by repositioning of genes to the nuclear lamina. Nature. Vol 452, doi:10.1038 /nature06727
Reddy, T.V. 1988. Mode of action of cobalt extending the vase life of cut roses. Scientia Horticulturae 36: 303-314
Sairam, R.K., Rao, K.V and Srivastava, G.C. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science, 163: 1037-1046
Scariot, V., Paradiso, R., Rogers, H and De Pascale, S. 2014. Ethylene control in cut flowers: Classical and innovative approaches. Postharvest Biology and Technology, 97:83-92. https://doi.org/10.1016/j. postharvbio.2014.06.010
Shahroodi, E., oise Bernard, F.C., Minaee Tehrani, D and Hassani, S.B. 2020. Stimulatory effect of salicylic acid on Polyamine oxidase activity in Thymus daenensis L. callus. Journal of Plant Research (Iranian Journal of Biology). 33(1): 111-118
Solgi, M., Kafi, M., Taghavi, T. S and Naderi, R. 2009. Essential oils and silver nanoparticles (SNP) as novel agents to extend vase-life of gerbera (Gerbera jamesonii cv. ‘Dune’) flowers. Postharvest Biology and Technology, 53(3):155–158. https://doi.org/10.1504/IJPTI.2011. 043325
Temel, A and Gozukirmizi,N. 2015. Physiological and Molecular Changes in Barley and Wheat Under Salinity. Journal of Applied Biochemistry and Biotechnology, 175:2950-2960
Terry, L.A and Joyce, D.C. 2004. Elicitors of induced disease resistance in postharvest horticultural crops: a brief review. Postharvest Biology and Technology 32:1-13
Van Meeteren, U. and Van Gelder, H. 1999. Effect of time since harvest and handling conditions on rehydration ability of cut chrysanthemum flowers. Postharvest Biology and Technology. 16: 169-177
Van Meeteren, U. 1978. Water relations and keeping-quality of cut Gerbera flowers. II. Water balance of ageing flowers. Scientia Horticulturae 9(2):189-197
Wan, Y., Wen, Ch., Gong, L., Zeng, H and Wang, Ch. 2023. Neoagaro-oligosaccharides Improve the Postharvest Flower Quality and Vase Life of Cut Rose ‘Gaoyuanhong’. HORTSCIENCE 58(4):404–409. https://doi.org/10.21273/HORTSCI16988-22
Wang, W., Chen, W., Luo, H., Jiang, L. and Yu, Z. 2016. Effect of salicylic acid on lignification of fresh-cut Zizania latifolia and the possible biochemical mechanisms. Journal of Food Engineering and Technology, 5:2
Xue, D., Botte, J., Deaets, B., Accoe, F., Nestler, A., Taylor, Ph., Van Cleemput, O., Berglund, M and Boeckx, P. 2009. Present limitations and future prospects of stable isotope methods for nitrate source identification in surface- and groundwater. Water Research. 43(5):1159-1170
Zeeshan, M., Lu, M., Sehar, Sh., Holford, P and Wu, F. 2020. Comparison of Biochemical, Anatomical, Morphological, and Physiological Responses to Salinity Stress in Wheat and Barley Genotypes Deferring in Salinity Tolerance. Agronomy, 10(1): 127; doi:10.3390/agronomy10010127
Zulfiqar, F., Nafees, M., Darras, A., Shaukat, N., Chen, J., Ferrante, A., Zaid, A., Latif, N., Raza, A and Siddique, K. 2020. Pre-harvest potassium foliar application improves yield, vase life and overall postharvest quality of cut gladiolus inflorescences. Postharvest Biology and Technology, 192: 112027. https://doi.org/10.1016/j.postharvbio.2022.112027