تغییر در رنگدانه و ترکیب رنگ گل با استفاده از تیمار هورمونی و ساکاروز در لاله رقم ’کینگز بلاد‘
Subject Areas : Journal of Ornamental Plantsیعقوب حجتی 1 , محمود شور 2 , علی تهرانی فر 3 , بهرام عابدی 4
1 - گروه باغبانی، دانشکده کشاورزی، دانشگاه مشهد، استان خراسان رضوی مشهد. کد پستی: 917751163
2 - گروه باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد
3 - استاد گروه علوم باغبانی و مهندسی فضای سبز دانشگاه فردوسی مشهد
4 - گروه باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد
Keywords: اسید جیبرلیک, فلاونوئید کل, اسید جاسمونیک, اسید آبسیزیک, رنگدانه آنتوسیانین,
Abstract :
آزمایشی بهمنظور بررسی اثر متقابل بین سه گروه از هورمونهای گیاهی و ساکارز برای تغییر ترکیب رنگ گل و متابولیتهای ثانویه گل لاله انجام شد. تغییرات مورفولوژیکی و فیزیولوژیکی گیاهان با استفاده از سه آزمایش جداگانه و توسط تیمار اسید آبسیزیک در غلظت 5 و 10 میلیگرم در لیتر، اسید جیبرلیک 300 و 500 میلی گرم در لیتر، اسید جاسمونیک 50 و 100 میکرو مولار و برهمکنش آنها با ساکاروز در غلظت 1 و 2 گرم در لیتر مورد ارزیابی قرار گرفت. با بررسی نمودارهای HPLCو طیفسنجی یوی دتکتور مشخص شد که تولید متابولیتهای ثانویه، فلاونوئید کل و ترکیب رنگدانههای آنتوسیانین تحت تاثیر تیمارهای هورمونی قرار گرفتند. با وجود اینکه تیمار ساکاروز به تنهایی قادر به تغییرات معنیدار در ترکیب و مقدار متابولیتهای ثانویه نبود، اما اثرات متقابل آن با تیمار هورمونی، اسید آبسیزیک و اسید جاسمونیک تغییرات معنیداری در میزان آنتوسیانینها و فلاونوئید کل ایجاد کرد. همچنین هر دو هورمون آبسیزیک اسید و جاسمونیک اسید تغییرات قابلتوجهی در افزایش میزان آنتوسیانینها و درصد رنگدانه سیانیدین و پلاگونیدین داشتند، اما در طرف دیگر سبب کاهش معنیدار در پارامترهای رویشی و عمر پس از برداشت گلها شد. در تیمار با اسید جیبرلیک 500 میلیگرم در لیتر بدون ساکاروز افزایش قابلتوجهی در میزان آنتوسیانین، عمر گل بریده و افزایش هر سه گروه آنتوسیانینی مشاهده شد. همچنین نتایج نشان داد بین اسید جیبرلیک و ساکاروز یک برهمکنش منفی در تنظیم تولید آنتوسیانینها وجود دارد.
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