Karyological Study of Domestic Goat (Capra Hircus) to determine of G-banded karyotype of Raeini goat
Subject Areas : Genetics
Mohammadsadegh Malekpour
1
(Veterinary department,azad university of shoushtar,shoushtar,iran)
Maryamolsadat Jananeh
2
(Veterinary Department, Faculty of Agriculture, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran)
Leila Roushani
3
(Veterinary Department, Faculty of Agriculture, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran)
Keywords: Goat, karyotype, Key Words: karyology, G-banding,
Abstract :
Abstract In order to prepare the karyotype of Raiini Goats from blood lymphocytes ,peripheral blood of 40Animals (20 male and 20 female) were taken from each breed and every genuse using heparinized Syring .Then ,all of the samples was transfered to laboratory and treat them in cool circumstance in 4degree of centigrade in refrigerator. After from each sample 0.5 ml of whole blood was cultured in RPMI1640 medium culture and incubated at 37ºC for 72 hours. The resulting (PHA-M) was used as mitogenic agent. After 72 hours 200µl cholchicine was added to each samples in order to stop the cell division and mitose cycle in metaphase stage. Then, they treated with hypotonic solution. Proliferated lymphocytes were spread and treated with trypsin enzyme, subsequently stained by G-banding method. All samples has monitored under microscope and pictures was sent to computer. The results showed that all goats have a diploid chromosome number of 2n=60. Each karyotype consisted of 29 pairs of acrocentric autosomes. The largest acrocentric chromosome was X and the smallest chromosome was Y.
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