بررسی چندشکلی ژنهای GDF9 و BMP15 در گوسفند مهربان برای یافتن جهشهای تأثیرگذار روی پروتیئن
Subject Areas : Camel
ا. احمدی
1
(
Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran
)
ف. افراز
2
(
Department of Genomics and Animal, Agricultural Biotechnology Research Institute (ABRI), North branch, Rasht, Iran
)
ر. طالبی
3
(
Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran
)
ع. فرح‌آور
4
(
Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran
)
س.م.ف. وحیدی
5
(
Department of Genomics and Animal, Agricultural Biotechnology Research Institute (ABRI), North branch, Rasht, Iran
)
Keywords: گوسفند, <, i>, BMP15<, /i>, , <, i>, GDF9<, /i>, , اندرکنش فیزیکی, واریانت, ساختار پروتئین,
Abstract :
بهرهگیری از ژنهای عمده باروری از قبیل GDF9 و BMP15 به بهبود صفات تولیدمثلی در برنامههای اصلاح نژادی گوسفند کمک شایانی خواهد نمود. به منظور ارزیابی جهشهای نقطهای بر روی عملکرد پروتئین، چندشکلی ژنهای GDF9 و BMP15 بر روی 12 گوسفند مهربان به وسیله توالی یابی DNA و مدلسازی ساختار پروتئین بررسی شد. تعداد شش چندشکلی تک نوکلئوتیدی شناخته شده به عنوان جهشهای FecG (G1-G6) در اگزون شماره 1 و 2 ژن GDF9 یافت شد. از میان چندشکلیهای یافت شده، جهش G1 (GDF9 exon1 g.2118 G>A)، G1 (GDF9 exon1 g.2118 G>A) و G6 (GDF9 exon 2 g.3974 G>A) ، جایگزینی اسیدآمینه را در ساختار پروتئین نشان دادهاند. هیچ چندشکلی در اگزون 1 و 2 ژن BMP15 یافت نشد. بر اساس چندشکلیهای شناسایی شده، افراد به سه دسته هاپلوتایپی از قبیل هاپلوتایپ وحشی (بدون جهش)، هاپلوتایپ A (جهشهای همزمان G1، G2، G3 و G4) و هاپلوتایپ B (جهشهای همزمان G5 و G6) تقسیمبندی شدند. ساختار سه بُعدی پروتئین GDF9 در هاپلوتایپ A و B به ترتیب یک چرخش 90 و 45 درجهای را نسبت به هاپلوتایپ نوع وحشی نشان داد. واریانتهای G1/p.Arg87His، G4/p.Glu241Lysو G6/p.Val332Ile بر روی پروتئین بیتأثیر بودند. در حالیکه دو جهشG7/p.Val371Met وG8/p.Ser315Phe به احتمال زیاد تأثیرات مخرب بر روی پروتئین داشتند. بر اساس آنالیز درخت تکامل ژن GDF9، افراد هاپلوتایپ A و B به ترتیب به ارزش خود راه اندازی 63 و 76 از هاپلوتایپ نوع وحشی فاصله داشتند. در نتیجه، پروتین GDF9 در افراد هاپلوتایپ نوع A و B به دلیل اثرات سینرژیسم جهشهای همزمان عملکرد بالاتری را نسبت به افراد هاپلوتایپ نوع وحشی نشان میدهد. این نوع جهشها با تأثیر بر روی مارپیچ و چرخشهای پروتئین GDF9 در نواحی حفاظتی، منجر به اندرکنش فیزیکی پروتئین GDF9 با پروتئینهای فاکتور تبدیل کننده رشد بتا (TGFβ) میشوند.
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