تأثیر سطوح مختلف مخمر زنده در خوراک با کنسانتره زیاد بر روی عملکرد، ترکیبات خونی و وضعیت سیستم ایمنی برههای زندی
Subject Areas : Camelم. راغبیان 1 , ا. بابایی یزدی 2 , ن. دبیری 3 , ا. حاجی محمدی 4 , پ. حاتمی 5 , ع. راغبیان 6 , ج. شمیزی 7 , م.ج. بحرانی 8
1 - Department of Animal Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Animal Science, Karaj Branch, Islamic Azad University, Karaj, Iran
3 - Department of Animal Science, Karaj Branch, Islamic Azad University, Karaj, Iran
4 - Department of Animal Science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
5 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
6 - Department of Animal Science, Saveh Branch, Islamic Azad University, Saveh, Iran
7 - Department of Animal Science, Karaj Branch, Islamic Azad University, Karaj, Iran
8 - Department of Animal Science, Karaj Branch, Islamic Azad University, Karaj, Iran
Keywords: عملکرد, سیستم ایمنی, وضعیت خونی, برههای زندی پرواری, کنسانتره زیاد, مخمر زنده,
Abstract :
مخمر به عنوان یک افزودنی خوراکی جهت بهبود عملکرد، سلامت حیوان و کاهش استفاده از آنتیبیوتیک در تولیدات دامی مورد استفاده قرار میگیرد. بنابراین آزمایشی با 27 بره نر از شیر گرفته زندی (با وزن اولیه 38/0±1/27 کیلوگرم) جهت بررسی تأثیر سطوح مختلف مخمر ساکارومایسز سرویسیه سویه SC47 در خوراک حاوی کنسانتره زیاد (85 درصد) بر روی عملکرد، پارامترهای خونی و وضعیت سیستم ایمنی انجام گرفت. برهها در 3 تیمار آزمایشی و در قالب یک طرح کامل تصادفی با 3 تکرار و 3 مشاهده در هر تکرار تقسیمبندی شدند. تیمارها شامل: 1. جیره پایه بدون مخمر (شاهد) 2. جیره پایه به همراه 3 گرم مخمر در روز به ازای هر بره (کم مخمر) 3. جیره پایه به همراه 5/4 گرم مخمر در روز به ازای هر بره (پر مخمر). مقایسه ماده خشک مصرفی نشان دهنده عدم وجود اختلاف معنیدار بین تیمارها تا پایان آزمایش بود (05/0P>). افزایش وزن روزانه در گروه پر مخمر بالاترین مقدار را در طول آزمایش داشت، اما تفاوتها بین تیمارها معنیدار نبود (05/0P>) و ضریب تبدیل غذایی نیز تأثیر معنیداری بین تیمارها نداشت. تفاوتها بین غلظت بعضی از نشانگرهای بیوشیمیایی پلاسما (پروتئین کل، گلوبولین و نسبت آلبومین به گلوبولین) معنیدار بود (05/0>P). بیشترین میزان پروتئین کل و گلوبولین در گروه کم مخمر مشاهده شد و تفاوت بین گروهها معنیدار بود (05/0>P)، به غیر از پروتئین کل در هفته دوازدهم (05/0P>). برههای گروه شاهد بیشترین نسبت آلبومین به گلوبولین را داشتند در حالیکه کمترین میزان آن در گروههای مصرفکننده مخمر مشاهده شد (05/0>P). اثر معنیداری در نتایج هماتولوژی مشاهده نشد (05/0P>). تفاوت معنیداری در گلبولهای سفید تشخیص داده نشد، به جز نوتروفیل باند که در گروه پر مخمر در هفته دوازدهم بیشترین مقدار را به خود اختصاص داد (05/0>P). نتیجه این که استفاده از مخمر زنده در خوراک حاوی کنسانتره زیاد میتواند عملکرد (به صورت غیر معنیدار)، غلظت نشانگرهای شیمیایی پلاسما (به صورت معنیدار) و پارامترهای هماتولوژی (به صورت غیر معنیدار) را در برههای زندی بهبود دهد.
Abo El-Nor S.A.H. and Kholif M.A. (1998). Effect of supplementation of live yeast culture in the diet on the productive performance of lactating buffaloes. Milchwissenschaft. 53, 663-666.
Amabile-Cuevas C., Cardenas-Garcia M. and Ludgar M. (1995). Antibiotic resistance. J. Anim. Sci. 83, 320-332.
Antunovic Z., Speranda M., Amidzic D., Seric V., Steiner Z., Doma-Cinovic N. and Boli F. (2006). Probiotic application in lambsnutrition. Krmiva. 4, 175-180.
Beeson W.M. and Perry T.W. (1952). Balancing the nutritional deficiencies of roughages for beef steers. J. Anim. Sci. 11, 501-509.
Brake J. (1991). Lack of effect of all live yeast culture on broiler, breeders and progeny performance. J. Poultr. Sci. 70, 1037-1039.
Bruno R.G.S., Rutigliano H.M., Cerri R.L., Robinson P.H. and Santos J.E.P. (2009). Effect of feeding Saccharomyces cerevisiae on performance of dairy cows during summer heat stress. Anim. Feed Sci. Technol. 150, 175-186.
DavisM.E., BrownD.C., Maxwell C.V., Johnson Z.B., Kegley E.B. and Dvorak R.A. (2004). Effect of phosphorylated mannans and pharmacological additions of zinc oxide on growth and immunocompetence of weaning pigs. J. Anim. Sci. 82, 581-587.
DeSmetI., Van Hoorde L., De Saeyer Van de Woeslyne M. and Verstraele W. (1994). In vitro study of bile salt hydrolase (BSH) activity of BSH isogonics Lactobacillus plantarum 80 strains and estimation of cholesterol lowering through enhanced BSH activity. Microbial Ecol. Health Dis. 7, 315-329.
Deaville E.R. and Galbraith H. (1992). Effect of dietary protein level and yeast culture on growth, blood prolactin and mohair fibre characteristics of British Angora goats. Anim. Feed Sci. Technol. 38, 123-133.
Demir G., Klein H.O., Mandel-Molinas N. and Tuzuner N. (2007). Beta glucan induces proliferation and activation of monocytes in peripheral blood of patients with advanced breast cancer. Int. J. Immunol. Pharmacol. 7, 113-116.
Desnoyers M., Giger-Reverdin S., Bertin G., Duvaux-Ponter C. and Sauvant D. (2009). Meta-analysis of the influence of Saccharomyces cerevisiae supplementation on ruminal parameters and milk production of ruminants. J. Dairy Sci. 92, 1620-1632.
Ding J., Zhou Z.M., Ren L.P. and Meng Q.X. (2008). Effect of monensin and live yeast supplementation on growth performance, Nutrient digestibility, carcass characteristics and ruminal fermentation parameters in lambs fed steam-flaked corn-based diets. Asian-Australas J. Anim. Sci. 21, 547-554.
Dobicki A., Preś J., Łuczak W. and Szyrner A. (2005). Influence of dried brewery’s yeast on body weight gains, physiological and biochemical indicators of blood and development of the rumen micro-organisms in calves. Med. Wet. 61, 946-949.
Dobicki A., Preś J., Zachwieja A., Mordak R. and Jakus W. (2007). Influence of yeast preparations on chosen biochemical blood parameters and the composition of cow milk. Med. Wet. 63, 955-959.
Erdman J.W. (1989). Phytic acid interactions with divalent cations in foods and in gastro intestinal tract. Pp. 161-170 in Mineral Absorption in Monogastric Gastro-Intestinal Tract. F.R. Dintizisand and J.A. Laszlo, Eds. Plenum Press, New York.
FAO. (2002). Probiotics in Food: Health and Nutritional Properties and Guidelines for Evaluation. Food and Agriculture Organization of the United Nations World Health Organization. Rome, Italy.
Fuller R. (1989). Probiotics in man and animals. J. Appl. Bacterial. 66, 365-374.
Fuller R. (1977). The Importance of lactobacilli in maintaining normal microbial balance in the crop. Br. Poult. Sci. 18, 84-94.
Fuller R. (1992). History and development of probiotics. Chapman and Hall, London, UK.
Galina M.A., Delgado M. and Ortíz M. (2006). Effect of a lactic probiotic on kids growth. Pp. 54 57th Ann. Meet. European Assoc. Anim. Prod. Wageningen, Netherlands.
Glade M.J. and Biesik L.M. (1986). Enhanced nitrogen retention in yearling horses supplemented with yeast culture. J. Anim. Sci. 62, 1635-1642.
Glade M.J. and Sist M.O. (1988). Dietary yeast culture supplementation enhances urea recycling in equine large intestine. Nutr. Reprod. Int. 37, 11-17.
Guedes C.M., Goncalves D., Rodrigues M.A.M. and Diasda-Silva A. (2008). Effects of a Saccharomyces cerevisiae yeast on ruminal fermentation and fibre degradation of maize silages in cows. Anim. Feed Sci. Technol. 145, 27-40.
Heinrichs A.J., Jones M. and Heinrichs B.S. (2003). Effects of mannan oligosaccharide or antibiotic in neonatal diets on health and growth of dairy calves. J. Dairy Sci. 86, 4064-4069.
Hernandez R., Gonzalez S.S., Pinos-Rodriguez J.M., OrtegaM.E., Hernandez A., Bueno G. and Cobos M. (2009). Effect of a yeast culture on nitrogen balance and digestion in lambs fed early and mature Orchard grass. J. Appl. Anim. Res. 35, 53-56.
Jouany J.P., Mathieu F., Senaud J., Bohatier J., Bertin G. and Mercier M. (1998). The effects of Saccharomyces cerevisiae and Aspergilus oryzae on the digestion of the cell wall fraction of a mixed diet in defaunated and refaunated sheep rumen. Reprod. Nutr. Dev. 38, 401-416.
Keyser S.A., McMeniman J.P., Smith D.R., MacDonald J.C. and Galyean M.L. (2007). Effects of Saccharomyces cerevisiae subspecies boulardii CNCM I-1079 on feed intake by healthy beef cattle treated with flor fenicol and on health and performance of newly received beef heifers. J. Anim. Sci. 85, 1264-1273.
Khalid M.F., Sarwar M., Mahr Un N. and Zia-Ur R. (2011). Response of growing lambs fed on different vegetable protein sources with or without probiotics. Int. J. Agric. Biol. 13, 332-338.
Line J.E., Bailey J.S., Cox N.A. and SternN.J. (1997). Yeast treatment to reduce Salmonella and Campylobacter population associated with broiler chickens subjected to transport stress. Poult. Sci. 76, 1227-1231.
MackieR.I., Mcsweeney C.S. and Klievea V. (2002). Microbiologyofthe rúmen. Pp. 95-118 in Sheep Nutrition. M. Freer and H. Dove, Eds. CSIRO Publishing, Canberra, Australia.
Małaczewska J. and Milewski S. (2010). Immunomodulating effect of Inter Yeast S on the non-specific and specific cellular and humoral immunity in lambs. Pol. J. Vet. Sci. 13, 163-170.
Mandour M.A., Al-ShamiS.A. and Altabari G. (2009). The effect of feeding probiotics on the productive performance of Saudi Arabia sheep breeds during. Mansoura Vet. Med. J. 11(1), 87-103.
Martin S.A., Nisbet B.J. and Dean R.G. (1989). Influence of a commercial yeast supplement on the in vitro ruminal fermentation. Nutr. Reprod. Int. 40, 395-403.
Masek T., Mikulec Z., Valpotić H., Kušće L., Mikulec N. and Antunac N. (2008). The influence of live yeast cells (Saccharomyces cerevisiae) on the performance of grazing dairy sheep in late lactation. Veterinarski Arhi. 78, 95-104.
Masek T., Mikulec H., Valpotic Snježan P., Stipetic B. and Perkic D. (2007). Influence of yeast culture (Saccharomyces cerevisiae) on performance of fattening lambs fed ground or whole grain diet. Krmiva. 4, 179-187.
Mikulec Ž., Mašek T., Habrun B. and Valpotić H. (2010). Influence of live yeast cells (Saccharomyce scerevisiae) supplementation to the diet of fattening lambs on growth performance and rumen bacterial number. Vet. Arhiv. 80(6), 695-703.
Miles R.D., Arafa I.S., Harms R.H., Carson C.W., Reid B.L. and Crawford J.S. (1981). Effects of a living non freeze dried lactobacillus acidophilus culture on performance, egg quality and gut microflora in commercial layers. Poult. Sci. 60, 993-1004.
Milewski S., Wójcik R., Małaczewska J., Trapkowska S. and Siwicki A.K. (2007). Effect of β-1.3/1.6-D-glucan on meat performance and non-specific humoral defense mechanisms in lambs. Med. Wet. 63, 360-363.
Milewski S., Brzostowski H., Tański Z., Zaleska B., Ząbek K. and Kosińska K. (2009). Effect of yeast preparations Saccharomyces cerevisiae on meat performance traits and hematological indices in sucking lambs. Pp. 101 in Proc. Ann. Meet. European Assoc. Anim. Prod. Wageningen, Netherlands.
Mohamadi P. and Dabiri N. (2012). Effects of probiotic and prebiotic on average daily gain, fecal shedding of Escherichia coli and immune system status in newborn female calves. Asian-Australas J. Anim. Sci. 25(9), 1255-1261.
Moore B.E., Newman K.E., Spring P. and Chandler F.E. (1994). The effect of yeast culture (Yea Sace 1026) in microbial population’s digestion in the cecum and colon of the equine. J. Anim. Sci. 72, 1-10.
Muchmore A.V., Sathyamoorthy N., Decker J. and Sherblom A.P. (1990). Evidence that specific high mannose oligosaccharides can directly inhibit antigen-driven T-cell responses. J. Leukoc. Biol. 48, 457-464.
Mukhtar N., Sarwar M., Nisa M.U. and Sheikh M.A. (2010). Growth response of growing lambs fed on concentrate with or without ionophores and probiotics. Int. J. Agric. Biol. 12, 734-738.
Newbold C.J., Wallace R.J. and Chen X.B. (1995). Differents trains of Saccharomyces cerevisiae differ in their effects on ruminal bacterial numbers in vitro and in sheep. J. Anim. Sci. 73, 1811-1818.
NRC. (1985). Nutrient Requirements of Poultry, 6th Rev. Ed. National Academy Press, Washington, DC., USA.
Onifade A.A. and Babatune G.M. (1996). Supplemental value of dried yeast in a high fiber diet for broiler chicks. Anim. Feed Sci. Technol. 62, 91-96.
Onifade A.A., Obiyan R.I., Onipede E., Adejumo O.A., Abu O.A. and Babatune G.M. (1999). Assessment of the effects of supplementing rabbit diets with a culture of Saccharomyces cerevisiae using growth performance, blood composition and clinical enzyme activities. Anim. Feed Sci. Technol. 77, 25-32.
Onifade A.A. (1997). Growth performance, carcass characteristics, organ measurements and hematology of broiler chickens fed a high fiber diet supplemented with antibiotics or dietary yeast. Die Nahrung. 41, 370-374.
Pagan J.D. (1990). Effect of yeast culture supplementation on nutrient digestibility in mature horses. J. Anim. Sci. 68, 371-380.
Rezaeian M. (2004). Effect of yeast culture supplementation on the performance of finishing Shal lambs. Proc. Br. Soc. Anim. Sci. 128, 211-121.
Sales J. (2011). Effectsof Saccharomyces cerevisiae supplementation on ruminal parameters, nutrient digestibility and growth in sheep: a meta-analysis. Small Rumin. Res. 100, 19-29.
Salminen S., Isolauri E. and Salminen E. (1996). Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful strains and future challenges. Antonievan Leeuwenhoek. 70, 347-358.
SAS Institute. (1997). SAS®/STAT Software, Release 6.11. SAS Institute, Inc., Cary, NC. USA.
Sharon N. (2008). Lectins: past, present and future. Biochem. Soc. Trans. 36, 1457-1460.
Shim S.B. (2005). Effects of prebiotics, probiotics and synbiotics in the diet of young pigs. Ph D. Thesis. WageningenUniversity and ResearchCenter, Wageningen, Netherlands.
Siwicki A.K., Kazuń K., Głąbski E., Terech-Majewska E., Baranowski P. and Trapkowska S. (2004). The effect of beta- 1.3/1.6-glucan in diets on the effectiveness of anti-Yersinia ruckeri vaccine-an experimental study in rainbow trout (Oncorhynchus mykiss). Pol. J. Food Nutr. Sci. 54, 59-61.
Spring P., Wenk C., Dawson K.A. and Newman K.E. (2000). The effects of dietary manna oligosaccharides on cecal parameters and the concentration of enteric bacteria in the ceca of Salmonella challenged broiler chicks. Poult. Sci. 79, 205-211.
Sykes A.R. (1978). An assessment of the value of plasma urea nitrogen and albumin concentrations as monitors of the protein status of sheep. Pp. 143-154 in The Use of Blood Metabolites in Animal Production. O.C.C. Publications, British, UK.
Thrune M., Bach A., Ruiz-Moreno M., Stern M.D. and Linn J.G. (2009). Effects of Saccharomyces cerevisiae on ruminal pH and microbial fermentation in dairy cows: yeast supplementation on rumen fermentation. Livest. Sci. 124, 261-265.
Williams P.W. (1989). Understanding the biochemical mode of action of yeast culture. Pp. 79-99 in Biotechnology in the Feed industry. T.P. Lyons, Ed. Alltech Technical Publications, Nicholasville, Kentucky.
Williams P.E., Tait C.A., Innes G.M. and Newbold C.J. (1991). Effects of the inclusion of yeast culture in the diet of dairy cows on milk yield and forage degradation and fermentation patterns in the rumen of steers. J. Anim. Sci. 69, 3016-3026.
Xiao Z., Trincado C.A. and Murtaugh M.P. (2004). Beta-glucan enhancement of T-cell IFN gamma response in swine. Vet. Immunol. Immunopathol. 102, 315-320.