شناسایی جایگاه های صفات کمی مؤثر بر صفات تولید شیر بر روی کروموزوم 14 در گاوهای شیری هلشتاین
Subject Areas : Camelم. نوری صادق 1 , س. انصاری-مهیاری 2 , م.پ. اسکندری نسب 3 , ف. رفیعی 4
1 - Department of Animal Science, College of Agriculture, University of Zanjan, Iran
2 - Department of Animal Science, College of Agriculture, Isfahan University of Technology, Isfahan, Iran
3 - Department of Animal Science, College of Agriculture, University of Zanjan, Iran
4 - Department of Agricultural Biotechnology, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
Keywords: QTL, تولید شیر, گاو هلشتاین ایران, LA, LDLA,
Abstract :
هدف از این تحقیق، تعیین جایگاه های صفات کمی (QTL) مؤثر بر صفات تولید شیر بر روی کروموزوم 14 گاوی با استفاده از دو روش تجزیه پیوستگی (LA) و عدم تعادل پیوستگی (LDLA) و مقایسه این دو روش در جمعیت هلشتاین ایران بود. تجزیه و تحلیل داده ها با آزمون نسبت درست نمایی (LRT) محاسبه و جهت برآورد مؤلفه های واریانس و کوواریانس بر اساس میانگین حداکثر احتمال اطلاعات از نرم افزار DMU استفاده و نتایج با آزمون مربع کای مقایسه شده و در نهایت محتمل ترین مکان ها برای صفات مورد نظر در سطح 5 درصد گزارش گردیدند. در این تحقیق تعیین ژنوتیپ بر مبنای طرح دختری با 232 دختر از 10 خانواده پدری و 10 نشانگر ریزماهواره در فاصله بین 0 تا 63 سانتیمورگان از نقشه، بین نشانگرهای ILSTS039 و DIK4361 (ILSTS011، DIK2598، DIK4884، DIK5080، CBDIKM002، ILSTS039، BM1508، CSSM066، CBDIKM004 و DIK4361) انجام گردید. برای صفات تولید شیر و تولید چربی در دو روش مورد استفاده به ترتیب، بیشترین معنیداری برای جایگاه QTL در فاصله های، 20 تا 60 و 54 تا 60 سانتیمورگان (با استفاده از LA) و در فاصله های 12 تا 60 و 60 سانتیمورگان (با استفاده از LDLA) گزارش شد. برای صفت درصد چربی شیر، بیشترین معنیداری برای جایگاه QTL در نقاط 3، 12، 20، 36، 44 و 50 سانتیمورگان در روش LA شناسایی شد. اما در روش LDLA برای صفت درصد چربی شیر معنی داری مشاهده نگردید. نتایج حاصل از این تحقیق با گزارشات مطالعات قبلی برای QTL های شناسایی شده مطابقت دارد. جهت شناسایی QTL در این تحقیق انتخاب به کمک نشانگر و انتخاب و تأیید ژن های کاندیدا مؤثر بر صفات اقتصادی در طرح های اصلاح نژاد گاو شیری می تواند مفید باشد.
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