شناسایی جایگاه های صفات کمی مؤثر بر صفات تولید شیر بر روی کروموزوم 14 در گاوهای شیری هلشتاین

Subject Areas : Camel

م. نوری صادق ¹ ( Department of Animal Science, College of Agriculture, University of Zanjan, Iran )
س. انصاری-مهیاری ² ( Department of Animal Science, College of Agriculture, Isfahan University of Technology, Isfahan, Iran )
م.پ. اسکندری نسب ³ ( Department of Animal Science, College of Agriculture, University of Zanjan, Iran )
ف. رفیعی ⁴ ( Department of Agricultural Biotechnology, Faculty of Agricultural Science, University of Guilan, Rasht, Iran )

Received: 2018-02-03 Accepted : 2018-05-15 Published : 2018-12-01

Keywords: QTL, تولید شیر, گاو هلشتاین ایران, LA, LDLA,

Abstract :

هدف از این تحقیق، تعیین جایگاه ­های صفات کمی (QTL) مؤثر بر صفات تولید شیر بر روی کروموزوم 14 گاوی با استفاده از دو روش تجزیه پیوستگی (LA) و عدم تعادل پیوستگی (LDLA) و مقایسه این دو روش در جمعیت هلشتاین ایران بود. تجزیه و تحلیل داده ­ها با آزمون نسبت درست ­نمایی (LRT) محاسبه و جهت برآورد مؤلفه ­های واریانس و کوواریانس بر اساس میانگین حداکثر احتمال اطلاعات از نرم افزار DMU استفاده و نتایج با آزمون مربع کای مقایسه شده و در نهایت محتمل­ ترین مکان­ ها برای صفات مورد نظر در سطح 5 درصد گزارش گردیدند. در این تحقیق تعیین ژنوتیپ بر مبنای طرح دختری با 232 دختر از 10 خانواده پدری و 10 نشانگر ریزماهواره در فاصله بین 0 تا 63 سانتی­مورگان از نقشه، بین نشانگرهای ILSTS039 و DIK4361 (ILSTS011، DIK2598، DIK4884، DIK5080، CBDIKM002، ILSTS039، BM1508، CSSM066، CBDIKM004 و DIK4361) انجام گردید. برای صفات تولید شیر و تولید چربی در دو روش مورد استفاده به ترتیب، بیشترین معنی­داری برای جایگاه QTL در فاصله­ های، 20 تا 60 و 54 تا 60 سانتی­مورگان (با استفاده از LA) و در فاصله ­های 12 تا 60 و 60 سانتی­مورگان (با استفاده از LDLA) گزارش شد. برای صفت درصد چربی شیر، بیشترین معنی­داری برای جایگاه QTL در نقاط 3، 12، 20، 36، 44 و 50 سانتی­مورگان در روش LA شناسایی شد. اما در روش LDLA برای صفت درصد چربی شیر معنی­ داری مشاهده نگردید. نتایج حاصل از این تحقیق با گزارشات مطالعات قبلی برای QTL های شناسایی شده مطابقت دارد. جهت شناسایی QTL در این تحقیق انتخاب به کمک نشانگر و انتخاب و تأیید ژن ­های کاندیدا مؤثر بر صفات اقتصادی در طرح ­­های اصلاح نژاد گاو شیری می ­تواند مفید باشد.

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