آنالیز توالی های تکراری ساده مشتق شده از توالی های بیان شده برچسب دار در شتر تک کوهانه
Subject Areas : Camelا. برازنده 1 , م. مختاری 2 , م. مقبلی دامنه 3 , ز. رودباری 4
1 - Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
3 - Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
4 - Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
Keywords: SSR, شتر, نشانگر مولکولی, ژنومی, EST, هستی‌شناسی ژن,
Abstract :
هدف از تحقیق حاضر بررسی ویژگی های توالی های تکراری ساده مشتق شده از توالی های بیان شده برچسب دار (نشانگرهای EST-SSR) در شتر تک کوهانه و همچنین تجزیه و تحلیل عملکردی این نشانگرها جهت استفاده در مطالعات مولکولی و ژنومی بود. از 17155 توالی EST با استفاده از نرم افزارSSR Locator تعداد 862 توالی تکراری ساده (SSRs) بدست آمد. به طورکلی تعداد 827 توالی EST دارای SSR بوده که 794 (96 درصد)، 31 (8/3 درصد) و 2 (2/0 درصد) تا از آنها به ترتیب حاوی 1، 2 و 3 توالی SSR بودند. موتیفهای دایمری فراوانترین نوع SSR بودند و پس از آنها به ترتیب موتیفهای تری، هگزا، تترا و پنتامری با 27.15، 21.46، 6.96 و 5.57 درصد قرار گرفتند. فراوانترین موتیف های دایمر، تریمر، تترامر، پنتامر و هگزامر به ترتیب AC/TG (54 درصد)، GCC/GGC (19.2 درصد)، TTTA (13.3 درصد)، AAAAG (10.4 درصد) و AACCAC (67.6 درصد) بودند. نشانگرهای EST-SSR نهایی با استفاده از BLASTX بررسی شدند. تقریباً اکثر این نشانگرها در فرایندهای کاتابولیک ماکرومولکول ها و پردازش و اسپلایسینگ RNA مشارکت داشتند. میتوان از نشانگرهای EST-SSR به عنوان نشانگرهای جدید مفید در بررسیهای بیولوژیکی استفاده نمود. همچنین این نشانگرها یک منبع ارزشمند جهت مطالعات مولکولی و ژنومی شتر و گونه های مرتبط با آن هستند.
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