ارتباط چندشکلی تک نوکلئوتیدی (SNP) g.281G > A ژن CAST با کیفیت گوشت بز بوئرکا

Subject Areas : Camel

ا.  آنتنیوس ¹ (Indonesia Goat Research Center Sei Putih, Galang 20585, North Sumatera, Indonesia)
س.پ.  گینتینگ ² (Indonesia Goat Research Center Sei Putih, Galang 20585, North Sumatera, Indonesia)
س.  الایزر ³ (Indonesia Goat Research Center Sei Putih, Galang 20585, North Sumatera, Indonesia)
ا.  تاریگان ⁴ (Indonesia Goat Research Center Sei Putih, Galang 20585, North Sumatera, Indonesia)
س.  سلهودین ⁵ (Indonesia Goat Research Center Sei Putih, Galang 20585, North Sumatera, Indonesia)
آی.گ.س.  بودیساتریا ⁶ (Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia)
آ.پ.ز.ن.ل.  ساری ⁷ (Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia)
د.ن.ه.  هاریونو ⁸ (Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia)
د.  ماهارانی ⁹ (Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia)

Received: 2019-06-18 Accepted : 2019-08-15 Published : 2020-06-01

Keywords: چندشکلی, کالپاستاتین, بز بوئرکا,

Abstract :

ژن کالپاستاتین به عنوان یک ژن کاندید برای کیفیت گوشت در گاو، گوسفند و مرغ شناخته شده است. هدف از این مطالعه شناسایی چندشکلی‌های ژن CAST و ارتباط آن با صفات گوشت در بز بوئرکا بود. داده‌های PH، از دست دادن پخت (CL)، نیروی برشی Warner-Bratzler (WBSF)، ظرفیت نگهداری آب (WHC)، کلسترول، آب، خاکستر، چربی، و محتوای پروتئینی رکورد برداری شدند. توالی‌یابی 21 نمونه، پنج چندشکلی تک‌نوکلئوتیدی در اینترون 12 ژن CAST در بز بوئرکا، به نام‌های g.146C > A، g.224A > G، g.281G > A، g.737C > T و g.431G > A نشان داد. تنها چندشکلی تک­نوکلئوتیدی (SNP) g.281G > A برای تعیین ژنوتیپ استفاده شد. فراوانی ژنوتیپی و آللی براساس g.281G > A نشان دهنده 14.29 درصد (ژنوتیپ GG) و 85.71 درصد (ژنوتیپ GA) همراه با 57 درصد آلل G و 43 درصد آلل A بود. آزمون کای-اسکوئر انحراف از تعادل هاردی-واینبرگ (HWE) (05/0P<) در بز بوئرکا را نشان داد. چندشکلی g.281G > A نشان دهنده دارا بودن اثر معنی‌دار بر CL بود. حیوانات با ژنوتیپ GA درصد CL کمتری در مقایسه با حیوانات GG داشتند. در نتیجه‌گیری، SNP انتخاب شده می‌تواند برای شناسایی گوشت دارای CL پایین در بز بوئرکا استفاده شود.

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