انتخاب مقادیر مناسب HLB برای امولسیون پایدار عسل-شی باتر و اثربخشی آن در درمان زخم سوختگی شیمیایی

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

جانسون ایساک ¹ ( گروه توسعه فناوری دارویی و مواد خام، NIPRD، قطعه 942، منطقه کاداستر C16، نیجریه )
عایشا دابوری ² ( گروه فارماکولوژی و سم شناسی، NIPRD، ابوجا، نیجریه )
کنچوکوو بن- اومه ³ ( گروه توسعه فناوری دارویی و مواد خام، NIPRD، قطعه 942، منطقه کاداستر C16، نیجریه )
بنت ایفانی ⁴ ( گروه توسعه فناوری دارویی و مواد خام، NIPRD، قطعه 942، منطقه کاداستر C16، نیجریه )
فیلیپ بیلدرز ⁵ ( گروه توسعه فناوری دارویی و مواد خام، NIPRD، قطعه 942، منطقه کاداستر C16، نیجریه )

Received: 2021-03-30 Accepted : 2021-08-06 Published : 2021-09-23

Keywords: Wound, Emulsion, Hydrophile-lipophile balance, Accelerated stability,

Abstract :

Background & Aim: Since ancient times till today, honey and shea butter are used in wound treatment. This present study aims to determine an effective hydrophile-lipophile balance (HLB) value that will produce a stable W/O honey-shea butter emulsion and to evaluate its healing potential on chemical burn wound in mice.Experimental: Using various ratios of span 20/tween 20, 6 formulations of honey-shea butter emulsion were prepared and subjected to accelerated stability study as per ICH guideline. The most stable preparation was further evaluated for its wound healing ability. The animals were sacrificed via chloroform inhalation after treatment for 7 days from wound created by dropping 0.2 ml, hydrochloric acid for 15 sec. The burn area was harvested and subjected to histopathological study.Results: Most stable emulsion was those prepare with ratio 5:5 of span 20/ tween 20. Haematoxylin-eosin and Verhoeff-Van Gieson stain revealed that groups treated with this emulsion had normal fibroblast, fibrocyte, epidermal cells, inflammatory cells, gland cells, collagen and elastic fibres; outperforming honey, shea butter and silver sulfadiazine groups. The present formulation gave a percent wound contraction of 36, while those of honey, shea butter, silver sulfadiazine were 29, 25 and 28, respectively. The occlusive effect of emulsion and the synergistic effect achieved by combining honey and shea butter could also be responsible for its superior wound healing activity.Recommended applications/industries: Our study concluded that honey-shea butter prepared with 5:5 span 20/tween 20 was relatively stable and possesses greater wound healing activity compared to commercial preparation and can safely be utilize as an effective natural therapy for burn wound.

References:

Alexandra, M.A., Joana, G., Angelo, Luis., Eugenia, G. and Ana, P.D. 2020. Evaluation of the in vitro wound-healing activity and phytochemical characterization of propolis and honey. Applied Sciences, 10: 1-14.

Ana, C.W., Talita, S.J., Dilmar, F.L., Mauricio, J.P., Gabriella, D.G. and Andrea, G.T. 2015. Comparative experimental study of wound healing in mice: Pelnac versus Integra. PLoS ONE, 10(3): 1-10.

Annalisa, M, V. 2020. Fat cells help wounds heal. AAAS Science Signalling, 13(639): 1-2.

Audu, Z., Assogba, G.A., Habib, G., Hope, S., Urbain, C.K., Roland, F.L., Sedami, M., Brigitte, E., Joelle, Q.L. and Ferdnad, A.G. 2020. Development of new dermatological formulation for the treatment of cutaneous candidiasis. Scientific African, 8: 1-8

Baccarin, T. andLemos, S.E. 2017. Potential application of nanoemulsions for skin delivery of pomegranate peel polyphenols. AAPS Pharm SciTech, 18: 3307-3314.

Davrieux, F., Allal, F., Piombo, G., Kelly, B. and Okulo, J. B. 2010. Near infrared spectroscopy of high- throughput characterization of Shea tree (Vitellaria paradoxa) nut fat profiles. Journal of Agricultural and food Chemistry, 58:7811-7819.

Hong, B.L., Yi, C., Mao-can, T., Xiao-hong, Y. and You-li, W. 2017. Effect of zicao ointment on second-degree burns in rats. Tropical Journal of Pharmaceutical Research, 16(4): 873-876.

Juttulapa, M., Piriyaprasarth, S. and Sriamornsak, P. 2013. Effect of pH on stability of oil-in-water emulsions stabilized by pectin–zein complexes. Advanced Materials Research, 747: 127-130.

Kamila, R., Yevgeniy, K., Zharylkasyn, Z., Kuat, K., Shiro, J. and Arman, S. 2021. Immunology of acute and chronic wound healing. Biomolecules, 11(700):1-25.

Laure, R., Dana, L.S., Jeffrey, S.O., John, J.V. and Gary, J.F. 2013. Eccrine sweat glands are major contributors to reepithelialisation of human wounds. The American Journal of Pathology, 182(1):163-171.

Lauren, E.T., Requel, A.M. and Caterson, E.J. 2016. Extracellular matrix and dermal fibroblast function in the healing wound. Advances in Wound Care, 5(3): 119-136.

Loden, M. and Andersson, A. C. 2008. Effect of topically applied lipids on surfactant-irritated skin. British Journal of Dermatology, 134(2): 215–220

Mohammed, H.I., Habiba, A., Momoh, S.A., Aliyu, M. and Rilwan, O.M. 2018. The cost of burn wound care in low-income nations could be reduced with the use of palm kernel oil: A preliminary study. Nigeria Journal of Plastic Surgery, 14(2): 17-21.

Nitish, B., Gursharan, K., Varinder, S., Juhi, K. and Ravi, K.D. 2016. Evaluation of the wound healing potential of isoquercetin-based cream on scald burn injury in rats. Burns and Trauma, 4(7):1-8.

Nur, A.G., Azree, E., Lee, C.Y., Nurul, Z.S., Srijit, D., Farihah, S., Rafizul, Y., Nurul, H.Y. andZar, C.T. 2016. Piper betel leaves induces wound healing activity via proliferation of fibroblasts and reducing 11β hydroxysteroid dehydrogenase-1-expression in diabetic rat. Journal of Ayurveda and Integrative Medicine, 30: 1-11.

Raham, F.E., Raham, I.A., Dalia, A. andElmazar, M.M. 2017. Honey-based hydrogel: in vitro and comparative in vivo evaluation for burn wound healing. Scientific Reports, 7(9): 1-11.

Rodrigo, C.O. and Steven, E.W. 2020. Fibrocytes, wound healing, and corneal fibrosis. Investigative Ophthalmology and Visual Science, 61(2): 1-14.

Samantha, E., Elaine, J.L. and Danielle, T. 2018. Immunology of wound healing. Current Dermatology Reports, 7: 350-358.

Serap, M., Eemel, C. and Pertevniyal, B. 2018. Herbal therapy for burns and burn scars. IntechOpen. 71665.

Subrahmanyam, M. 2007. Topical application of honey for burn wound treatment-an overview. Annals of Burns and Fire Disasters, 20(3): 137-139.

Tzu-Kai, L., Lily, Z. and Juan, L.S. 2018. Anti-inflammatory and skin barrier repair effects of topical application of some plant oils. International Journal of Molecular. Sciences, 19(70): 1-21.

Verma, N., Chakrabarti, R., Das, R.H. and Guatam, H.K. 2012. Anti-inflammatory effects of shea butter through inhibition of iNos, COX-2, and cytokines via the NF-k b pathway in LPS-activated J774 macrophage cells. Journal of Complementary and Integrative Medicine, 9:1.

Vijay, K.K., Arvind, M.P., Joakim, H., Jan, N., Robert, S., Sarunas, P. and Suchitra, S.H. 2017. Significantly accelerated wound healing of full-thickness skin using a novel composite gel of porcine acellular dermal matrix and human peripheral blood cells. Cell Transplantation, 26:293-30.

Yaghoobi, R., Kazerouni, A. and Kazerouni, O. 2013. Evidence for clinical use of honey in wound healing as an anti-bacterial, anti-inflammatory anti-oxidant and anti-viral agent: a review. Jundishapur Journal of Natural Pharmaceutical Products, 8(3): 100-114.

Yaman, A.S., Durmus, S. andCeribasi, M.Y. 2010. Effects of Nigella sativa and silver sulfadiazine on burn wound healing in rats. Veterinarni Medicina, 55(12): 619-624.