Investigation of the inhibitory effect of engineered probiotic Lactobacillus acidophilus on the growth of Salmonella enteritidis
Subject Areas : Food Microbial Contamination
Mottahareh Eskandari
1
,
Abbas Doosti
2
1 - IAU,,.shahrekord Branch
2 - Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Keywords: Salmonella, Lactobacillus acidophilus, Tachyplesin,
Abstract :
Probiotics are living cellular components that have beneficial effects on the host in a certain number when they enter the gastrointestinal tract. The aim of this study was to produce and evaluate the effects of the probiotic bacterium Lactobacillus acidophilus, which expresses the peach's peptide Tachyplesin I, on the growth of Salmonella. The recombinant plasmid pNZ8148- Tachyplesin containing the Tachyplesin gene was introduced into E. coli by heat shock and then purified. The recombinant vector containing the tachiplicin gene was then inserted into Lactobacillus acidophilus by electroporation method and screened by chloramphenicol antibiotic and PCR. Then, the inhibitory effect of engineered Lactobacillus acidophilus on the growth of Salmonella was investigated and compared with antibiotic discs..After confirmation of the entry of plasmid pNZ8148-Tachyplesin into Lactobacillus acidophilus, it had an inhibitory effect on the growth of Salmonella pathogenic bacterium as the diameter of the growth inhibition zone created on Salmonella was acceptable under the influence of engineered Lactobacillus. After comparing the diameter of the auras of ampicillin, chloramphenicol and tetracycline antibiotic disks with engineered lactobacillus and performing statistical analysis, no significant difference was reported. Considering that the presence of Tachyplesin gene in Lactobacillus acidophilus was confirmed, the resulting growth inhibition zone around Salmonella indicates that the expression and secretion of Tachyplesin gene in Lactobacillus has an antibiotic-like effect on Salmonella.
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