Survey on antibacterial effect of Padina sp. and Sargassum sp. collected from the coastal areas of Bushehr port on Vibrio cholera and Escherichia coli isolated from water

Subject Areas : Food Microbial Contamination

Alireza Golchin Manshadi ¹ , برهان مال الهی ² , محمد ترحمی ³

1 - عضو هیات علمی دانشگاه آزاد اسلامی واحد کازرون
2 - دانشگاه آزاد اسلامی واحد کازرون
3 - دانشگاع آزاد اسلامی واحددکازرون

Received: 2015-05-10 Accepted : 2015-07-21 Published : 2015-08-01

Keywords: MIC, Sargassum sp, MBC, Padina sp,

Abstract :

To evaluate the antibacterial effect of Padina sp. and Sargassum sp. extracts, collected from coastal areas of Bushehr port, on Vibrio cholera and Escherichiacoli, after washing in distilled water, the Padina sp. and Sargassum sp. were dried. Extraction was performed by immersion in 96% methanol to obtain methanol extract. Serial dilutions of the algal extracts were prepared by methanol and DMSO to find effect of solvent on algea potential as pure (100 percent), 25 percent and finally 12.5 to 0.195 percent. Concentration of bacteria for inoculating in tubes were prepared base on McFarlane turbidity and was used to determine Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of algal extracts. The results showed that both Padina sp .and Sargassum sp. were effective on V. cholera and E. coli, although the results were not statistically significant (P>0.05). However the effect of Padina sp. on both bacteria and methanol extract was more. The relationship between the concentration of algal extracts with MIC and MBC based on linear regression method showed that there is a significant relationship between two parameters (P≤0.05).