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        1 - Alternations of Antioxidant Activity in Saliva in Smokers
        S. F. Nosratabadi R. Sariri P. Yaghmaei M. Taheri A. Ghadimi H. Ghafoori
        Cigarette smoke is injurious to both oral cavity and internal body environment. Saliva is the firstbody fluid to encounter the dangerous smoke. The aim of our present study was, therefore, toevaluate the influence of smoking on the antioxidant activity of saliva of heal أکثر
        Cigarette smoke is injurious to both oral cavity and internal body environment. Saliva is the firstbody fluid to encounter the dangerous smoke. The aim of our present study was, therefore, toevaluate the influence of smoking on the antioxidant activity of saliva of healthy smokers.Antioxidant capacity of saliva was determined using different methods in the centrifuged saliva from25 male smokers and 25 non-smokers in a similar age range. Chemical methods and highperformance liquid chromatography (HIQC) revealed a significant decrease in total antioxidantcapacity and values of non-enzymatic antioxidants in saliva of smokers. Based on the results obtainedin the present study, we concluded that smoking could significantly affect the anitoxidant behavior ofnormal human saliva. Thus, smoking may induce several oral diseases as well as respiratory andcardiovascular disorders and could cause different cancers. تفاصيل المقالة
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        2 - The organic extracts from the leaves of <i>Ficus thonningii</i> Blume, <i>Jatropha tanjorensis</i> J.L Ellis and Saroja and <i>Justicia carnea </i> Lindley as potential nutraceutical antioxidants and functional foods
        Kingsley Ijoma Vincent Ishmael Ajiwe Stanley Odinma
        The search for naturally occurring plant-based nutraceutical antioxidants is imperative in the management of arrays of diseases. Herein, the methods involved profiling antioxidant activities and nutritional composition of the methanolic extracts from the leaves of Ficus أکثر
        The search for naturally occurring plant-based nutraceutical antioxidants is imperative in the management of arrays of diseases. Herein, the methods involved profiling antioxidant activities and nutritional composition of the methanolic extracts from the leaves of Ficus thonningii Blume (FTH), Jatropha tanjorensis J.L Ellis and Saroja (JTR) and Justicia carnea Lindley (JCN) using spectrophotometric techniques. In this sense, J. tanjorensis J.L Ellis and Saroja extract showed the highest total antioxidant capacity and strong DPPH radical scavenging activity with an IC50 of 10.490 &plusmn; 0.0320 &mu;g.mL-1 when compared to ascorbic acid (IC50 of 114.140 &plusmn; 5.135 &mu;g.mL-1), while for the hydroxyl radical scavenging activity, F. thonningii Blume and J. carnea Lindl. extracts resembled IC50 values of 523.095 &plusmn; 13.629 and 503.733 &plusmn; 7.562 &mu;g.mL-1, respectively. A positive correlation was observed between the extracts and ascorbic acid (p &lt; 0.001), as well. In addition, nutrients and minerals were present in appreciable quantities. The obtained results validate the assayed plants extracts as potential radical scavengers and promising nutraceutical antioxidants in ethnomedicine. تفاصيل المقالة
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        3 - Enrichment of polyphenolic compounds from grape leaf extract (<i>Vitis vinifera</i> L.) using macroporous resin and investigating its adsorption kinetics and dynamics
        Nadia Sobhani Mahshad Shahriari Mohammad Hossein Jabbari Samad Nejad Ebrahimi
        Vitis vinifera L. with common name of grape is native to Mediterranean region and today is grown all over the world.آHere, the adsorption/desorption process on LXA-10 resin was investigated to enrich polyphenolic compounds of grape extract. The extract was obtained usin أکثر
        Vitis vinifera L. with common name of grape is native to Mediterranean region and today is grown all over the world.آHere, the adsorption/desorption process on LXA-10 resin was investigated to enrich polyphenolic compounds of grape extract. The extract was obtained using ultra-sound-assisted probe with water and ethanol (50:50) with an extraction yield of 26.4%. The pH value of the extract for maximum adsorption was optimized at 3.26. According to the findings of this study, the kinetic adsorption fitted well with pseudo-second-order model (R2=0.99). Moreover, the dynamic study revealed the adsorption capacity and required solvent for polyphenolic desorption being 42 mL and six-bed volume (BV), respectively. In the enriched powder, the amount of quercetin-O-3-glucuronide and quercetin-O-3-glucoside increased from the ranges of 19.43 to 45.92 mg.g-1 and 41.244 to 72.88 mg.g-1, respectively. The antioxidant activity of enriched polyphenols also increased from 252.37 in initial grape extract to 408.03 mM iron (II).mg-1. تفاصيل المقالة
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        4 - Comparison of DPPH and FRAP Assays for Determination of Antioxidant Activity of electro-synthesized Polypyrrole /graphene nanocomposite
        nafiseh hajiabdolah Mohammad Yousefi
        This study is in investigated the antioxidant effects of polypyrrole and grapheme nanocomposite on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and Ferric reduction activity potential (FRAP). The electrosynthesis of this nanocomposite on the tip of a graphite penci أکثر
        This study is in investigated the antioxidant effects of polypyrrole and grapheme nanocomposite on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and Ferric reduction activity potential (FRAP). The electrosynthesis of this nanocomposite on the tip of a graphite pencil by cyclic voltammetry (CV) in the potential range -0.4 to 1.2V at the 5 mL of phosphoric acid (H3PO4), in the presence of potassium chloride (KCl) electrolyte supplements as an inorganic salt and p-Toluene sulfonic acid (pTSA) as an organic salt was performed. Antioxidant capacity was determined using Uv-vis spectroscopy. Also, FT-IR Spectroscopy and scanning electron microscopy (FESEM) were used to study the surface structure and topography of nanocomposite.Polypyrrole-graphene nanocomposite has antioxidant properties and increases the absorption rate at FRAP = &lambda;max596 nm by reducing iron ions and reducing the absorption rate in DPPH = &lambda;max516 nm by the transition of electrons and destroying DPPH free radicals. Also, this nanocomposite in the presence of p-Toluene sulfonic acid has a higher antioxidant capacity than the presence of potassium chloride. تفاصيل المقالة
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        5 - Antimicrobial and antioxidant potentials of callus cultures of Convolvulus microphyllus Sieb. ex Spreng.
        بوسکی پانچولی ام گوتام
        Background &amp; Aim: Convolvulus microphyllus Sieb. ex Spreng. (Family: Convolvulaceae) known as &ldquo;Shankhpushpi&rdquo; is reported to be brain tonic and useful in CNS disorder, hypertension, thyrotoxicosis and ulcer. Tissue culture technique has been optimized for أکثر
        Background &amp; Aim: Convolvulus microphyllus Sieb. ex Spreng. (Family: Convolvulaceae) known as &ldquo;Shankhpushpi&rdquo; is reported to be brain tonic and useful in CNS disorder, hypertension, thyrotoxicosis and ulcer. Tissue culture technique has been optimized for the large scale production of medicinally important plant independent from climatic and geographic conditions. Antioxidant and antimicrobial activities were performed and compared with in vivo. Active compounds with biological activities were isolated, identified from the callus extracts. These biological constituents can be propagated in large amount with low cost use tissue culture techniques.Experimental: Cell cultures of C. microphyllus have been established using Murashige and Skoog&rsquo;s (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2, 4-D). Callus was harvested at different time intervals of 2, 4, 6 and 8 weeks and their antimicrobial and antioxidant potentials along with the isolation of active compounds isolation were carried out using established protocols.Results: Ethanolic extracts of 2 weeks-old callus demonstrated appreciable antifungal activity against Penicillium chrysogenum and Tricophyton rubrum (inhibition zone of 14.66 &plusmn; 0.66 and 14.00 &plusmn; 0.57 mm respectively) while maximum antibacterial activity was recorded in 6 and 4 weeks-old callus against Klebsiella pneumoniae (Inhibition zone of 14.66 &plusmn; 0.61 mm and 14.33 &plusmn; 0.59 mm respectively). Antioxidant potentials were more in plant extract (IC50 0.055 mg/ml and 510 &plusmn; 20.02 ascorbic acid equivalents) as compared to callus. Phenolic acids viz., caffeic-, p- coumaric-, ferulic-, gallic-, vanillic- and syringic acids were isolated and screened for antimicrobial efficacy.Recommended applications/industries: The callus extract shows similar results as that of In vivo plant. Two week old callus exhibit most profound antifungal and 4 to 6 week for antimicrobial activities. Callus extract shows similar bio-potentials and secondary metabolites level, so it can be used for large scale production of biologically active phytochemicals with antimicrobial properties. تفاصيل المقالة
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        6 - α-Amylase inhibition and antioxidant activity of Singhnad Guggul in vitro
        اشوک کومار سالیمولا خان گوپی ستی ساران ناوین کومار لوکانت وی
        Background &amp; Aim: Singhnad Guggulu is a traditional Ayurvedic formula for detoxifying and rejuvenating the joints. The present study was to investigate the &alpha;-amylase inhibition and antioxidant activities of aqueous extract of Singhnad Guggul (AESG). Experimen أکثر
        Background &amp; Aim: Singhnad Guggulu is a traditional Ayurvedic formula for detoxifying and rejuvenating the joints. The present study was to investigate the &alpha;-amylase inhibition and antioxidant activities of aqueous extract of Singhnad Guggul (AESG). Experimental: &alpha;-Amylase inhibition assay by dinitro-salicylic acid method and antioxidant activity by ferric chelating and ferrous reducing assay. Results: AESG indicated that potent &alpha;-amylase inhibition activity (IC50= 84.00 &micro;g/ml). AESG also showed ferric chelating and ferrous reducing activities. Recommended applications/industries: Our studies showed that Singhnad Guggulu possess alpha- amylase and antioxidant properties. تفاصيل المقالة