شناسایی مولکولی ژنهای مقاومت به انروفلوکساسین دراشریشیا کلی جدا شده از ماکیان گوشتی در تهران و تنکابن
محورهای موضوعی : پژوهش های بالینی دام های بزرگ
کلید واژه: انروفلوکساسین, مقاومت آنتی بیوتیکی, اشریشیا کلی, DNA ژیراز,
چکیده مقاله :
اشریشیا کلی یکی ازشایع ترین عوامل بیماریزا درطیور می باشد که منجر به بیماری کلی باسیلوز می‌گردد.این بیماری مسئول ضرروزیان اقتصادی برای تولید کنندگان مرغ در سراسر جهان می‌باشد. فلورکینولون ها به خصوص انروفلوکساسین که نسل دوم فلورکینولون‌ها می‌باشند به طور گسترده ای در مرغداری ها جهت درمان بیماری مورد استفاده قرار می گیرد.استفاده وسیع و بی رویه از فلورکینولون ها در صنعت طیور خطر ایجاد مقاومت آنتی بیوتیکی در بین اشریشیا کلی بیماریزا را افزایش می دهد.DNA ژیراز (توپوایزومرازII ) و توپوایزومراز IV هدف اصلی فلورکینولون‌ها می‌باشد. جهش درژن های کد کننده DNA ژیراز (gyrA, gyrB) و توپوایزومراز IV (ParC , parE) علت اصلی مقاومت به فلورکینولون ها در اشریشیا کلی می باشد.هدف ازاین مطالعه شناسایی جهش‌های مرتبط با ژنهای دخیل در مقاومت آنتی بیوتیکی به انروفلوکساسین می باشد.از 80 جدایه E.coli ،20 جدایه توسط روش دیسک دیفوژن، مقاوم به انروفلوکساسین شناسایی شدند . سپس حضور جهش درژن های parC,gyrB, gyrA parE, درآنها مورد بررسی قرار گرفت .جهش های ,S83®L D87®NدرF514®S ,gyrA درS80®I ,gyrB در parC مشاهده شد اما جهشی که منجر به تغییر در اسیدآمینه شود در parE یافت نشد.
E.coli is one of the most prevalent pathogenic factor in poultry which causes colibacillosis. This disease is responsible for lots of economic losses that happen for poultry industry all around the world. Fluoroquinolones especially enrofloxacin is widely used as treatment for the disease in poultry farms. Having anomalous and vast usage of fluoroquinolones in poultry industry would increase antibiotic resistance risk inpathogenic E.coli. DNA gyrase (topoisomerase II) and topoisomerase IV are the main targets for fluoroquinolones. Mutation in encoding genes of DNA gyrase and topoisomerase can be considered as the main cause in resistance to fluoroquinolones in E. coli. The objective of the present study was to identify associated mutation interfering genes to enrofloxacin. Among 80 genera of E.coli there were recognized just 20 through diffusion disk method resistant against enrofloxacin. Then we considered the existence of mutants in genes:gyrA, gyrB, parC and parE. Some mutations like D87→N, S83→L were recognized in gyrA also F514→S in gyrB , S80 →I in parC but no mutation that leading to exchange was seen in parE.
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