بررسی ایمنی زایی واکسن روغنی تب برفکی فرموله شده با ادجوان های روغنی در مقایسه با ادجوان ژل آلومینوم هیدروکساید در بز
محورهای موضوعی : ویروس شناسی
مسعود مقدم پور
1
,
سید محمود عظیمی
2
1 - بخش تحقیق و تولید واکسن های ویروسی ، موسسه تحقیقات واکسن و سرم رازی، سازمان تحقیقات کشاورزی، آموزش و ترویج (AREEO)، کرج، ایران
2 - بخش تحقیق و تولید واکسن های تب برفکی ، موسسه تحقیقات واکسن و سرم رازی، سازمان تحقیقات کشاورزی، آموزش و ترویج (AREEO)، کرج، ایران
کلید واژه: واکسن روغنی, تب برفکی, ژل آلومینیوم هیدروکساید, بز , ادجوان روغنی ,
چکیده مقاله :
در مناطقی که بیماری تب برفکی اندمیک می باشد، همانند ایران، موثرترین راه کنترل و پیشگیری از بیماری واکسیناسیون است. در حال حاضر واکسن تب برفکی در دو طیف پایه آبی و روغنی بصورت تجاری تولید شده و در اختیار دامداران قرار دارد. در مطالعه حاضر میزان پاسخ ایمنی همورال و مدت دوام آن متعاقب تجویز واکسن مورد بررسی قرار گرفت. در این تحقیق واکسن های آزمایشی با ادجوان روغنی 62B با واکسن دارای ادجوان ISA61 و واکسن دارای ژل آلومینیوم هیدروکساید در دام هدف (بز) مورد مقایسه قرار گرفت. واکسیناسیون گروه ها در دو نوبت و با فاصله 28 روز انجام شد. نمونه گیری از دام های تحت آزمایش در روز های صفر، 28، 60، 90، 120، 150، 180، 210 انجام شد. بررسی ایمنی همورال دام ها با استفاده از روش خنثی سازی سرم انجام شد. عیار آنتی بادی حاصل از واکسن های دارای ادجوان های روغنی 62 B و ISA61 و ژل آلومینیم هیدروکساید تا روز 60 افزایش یکسانی را نشان دادند ولی از روز 60 عیار سرمی گروه دریافت کننده ژل آلومینیوم هیروکساید کاهش یافت ولی دو گروه های که ادجوان 62 B و ISA61دریافت کرده بودند تا روز 210 عیار خنثی سازی بالاتر و یا حدود 6/2 نشان دادند. بررسی های آماری نتایج عیار سرمی در آزمایش خنثی سازی سرم( SN) اختلاف معنی دار را بین گروه واکسن های دارای ادجوان های روغنی 62 B و ISA61 با سایر گروه ها از روز 60 تا پایان تحقیق را نشان داد .
Foot and mouth disease is one of the infectious diseases in the livestock industry. In areas where foot-and-mouth disease is endemic the most effective way to control and prevent the disease is vaccination. At present, foot-and-mouth disease vaccine is commercially produced in two types of Alum and oil-based and is available to livestock farmers. In the present study, the level of humoral immune response and its duration following the administration of a vaccine prepared with an internal oil adjuvant compared to a vaccine with an imported adjuvant and a vaccine with aluminum gel in target animals (goats) were investigated. two occasions with an interval of 28 days vaccination were done. Sampling was done on days zero, 28, 60, 90, 120, 150, 180, 210. The humoral immunity was investigated using serum neutralization method. Satistical methods were used in order to determine the significant difference in results of groups . The antibody titer obtained from group A and group B and group C showed the same increase until day 60, but from that time the serum titer of group C decrease , but the other groups(A&B) kept the titer obtained until day 210. The groups D and E during the research period could not have the good titer and quickly lost the low titer obtained on the 60th day. Statistical analysis of the results of serum resalts in the serum neutralization test (SN) showed a significant difference between the group of vaccines with oil adjuvants 62 B and ISA61 and other groups from the 60th day to the end of research. The neutralization rate of the above-mentioned two groups was reported to be about 2.6.The groups C and D performed very poorly in increasing the immunity level and also the length of the immunization period compared to the groups A and B. In total and considering the lack of decrease in the immunity level after 210 days (7 months) , in Group (B 62) where foot-and-mouth oil vaccine contained internal adjuvant was inoculated, compared to other foot-and-mouth vaccines, they can be of great help to the livestock industry to deal with foot-and-mouth disease.In total, considering the increase in the appropriate immunity rate up to 210 days (7 months) in the first group (B 62) which had a domestically produced adjuvant, compared to other groups, this type of vaccine can be great help to control FMD in animal husbandry industry.
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