بررسی اثر متادون بر طول عمر تا نسل چهارم، باروری، سطح مالون دی آلدئید کل بدن و آسیب‌های بافتی مغز در نسل اول مگس‌های سرکه
محورهای موضوعی : زیست شناسی
زهرا رستمی
1
,
مجید حسن پورعزتی
2
1 - کارشناسی ارشد، گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه شاهد، تهران، ایران
2 - استادیار، گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه شاهد، تهران، ایران
کلید واژه: متادون, مگس سرکه, توارث, مالون‌‌دی‌‌آلدئید, باروری, طول عمر, مغز.,
چکیده مقاله :
هدف: مستندات اولیه نشان می‌دهند که مصرف مواد افیونی می‌تواند بر طول عمر و عملکرد دستگاه تولید مثلی تاثیر بگذارد. در این مطالعه، اثر مصرف خوراکی متادون توسط والدین و نسل اول بر سطح مالون دی آلدئید کل بدن، آسیب‌های بافت مغز در نسل اول، و طول عمر، و باروری مگس‌ها و انتقال اثر تا چهار نسل بررسی شده است.
مواد و روش‌ها: در دوره تخم‌گذاری و نسل اول، مگس والد (10 عدد از هر جنس) تحت درمان خوراکی با متادون (0.1، 1، 5، 10 و 100 میکرومول) و گروه کنترل مثبت تحت درمان با مترونیدازول (1 و 2 میلیگرم در لیتر) قرار گرفتند. تعداد مگس‌های زنده، و فرزندان در هر گروه حداکثر برای 94 روز به صورت هفتگی شمارش شدند. غلظت مالون دی آلدئید در هموژنای کل بدن (30 عدد از هر جنس) به کمک کیت تجاری اندازه‌گیری شد. نواحی فاقد سلول در برش‌های بافت مغز پس از رنگ‌آمیزی هماتوکسیلین- ائوزین شمارش شدند.
یافته‌ها: مصرف دوزهای 10 و 100 میکرومول متادون منجربه مرگ و دوز 5 میکرومول باعث عقیمی کامل مگس‌ها شد. طول عمر و تعداد فرزندان مگس‌ها به صورت وابسته به جنس، بدنبال مصرف خوراکی دوزهای 0.1 و 1 میکرومول در لیتر متادون کاهش یافت. محتوای مالون دی آلدئید فقط در مگس‌های نر تحت اثر متادون
(1 میکرومول) به 0.6 میکرومول/میلیگرم افزایش یافت و تعداد مناطق بدون سلول در یک مقطع تهیه شده از بافت مغز به 60 عدد در مقایسه با گروه کنترل 15 عدد افزایش یافت. اثرات مشاهده شده در مگس‌های ماده، متفاوت از نر بود.
نتیجه‌گیری: مصرف متادون توسط والدین و نسل اول مگس‌ها از طریق تحریک تولید محصول پراکسیداسیون چربی‌ها در بدن و آسیب مغزی باعث کاهش طول عمر و کاهش تولید مثل در نسل اول و انتقال آن به نسل بعدی می‌شود.
Objective: Preliminary documentation shows that the use of opioids can affect
the lifespan and function of the reproductive system. In this study, the effect of oral methadone consumption by parents and the first generation on the level of malondialdehyde in the whole body, brain tissue damage in the first generation, and life span, and the fertility of flies and the transmission of the effect up to four generations have been investigated.
Materials and methods: During the laying period and the first generation,
parent flies (10 of each sex) were treated orally with methadone (0.1, 1, 5, 10 and 100 micromol) and the positive control group was treated with metronidazole (1 and 2 mg in liters) were placed. The number of live flies and offspring in each group were counted weekly for a maximum of 94 days. The concentration of malondialdehyde was measured in whole body homogenates (30 of each gender) using a commercial kit. Areas without cells were counted in brain tissue sections after hematoxylin-eosin staining.
Findings: 10 and 100 micromol doses of methadone led to death and 5 micromol doses caused complete sterility of flies. The life span and the number of offspring of flies in a sex-dependent manner decreased after oral administration of doses of 0.1 and 1 μmol/L methadone. The content of malondialdehyde increased to 0.6 μmol/mg only in male flies under the effect of methadone (1 μmol), and the number of cell-free areas in a section prepared from brain tissue increased to 60 compared to 15 in the control group. The effects observed in female flies were different from males.
Conclusion: Using methadone by the parents and the first generation of flies by stimulating the production of fat peroxidation products in the body and brain damage reduces the lifespan and reduces reproduction in the first generation and its transfer to the next generation.
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