اثر ضدسرطانی عصاره هیدروالکلی گیاه گل انگشتانه (Digitalis nervosa) بر رده سلولی سرطان دهانه رحم (Hela)
محورهای موضوعی :
گیاهی
نازیلا احمدزاده
1
(کارشناسی ارشد، گروه زیست‌شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران.)
نسترن اصغری مقدم
2
(استادیار، گروه زیست‌شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران)
زهرا کشتمند
3
(استادیار، گروه زیست‌شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران)
تاریخ دریافت : 1401/01/08
تاریخ پذیرش : 1401/03/11
تاریخ انتشار : 1401/04/01
کلید واژه:
رده سلولی,
زنده مانی,
MTT,
عصاره هیدروالکلی,
گل انگشتانه,
رده سلولی Hela,
آپوپتوز,
سرطان دهانه رحم,
چکیده مقاله :
هدف: امروزه با شیوع زیاد سرطان، نیاز به داروهایی با عوارض جانبی کمتر و اثرات درمانی بهتر مورد توجه پژوهشگران قرار گرفته است، به طوری که درصد زیادی از ترکیبات ضد سرطانی، از منابع طبیعی تهیه می‌‌شوند. هدف پژوهش حاضر بررسی اثرات ضدسرطانی عصاره هیدوالکلی گیاه گل انگشتانه (Digitalis nervosa) بر رده سلول سرطان رحم (Hela) می‌‌باشد.مواد و روش‌ها: در این مطالعه تجربی، عصاره هیدروالکلی گیاه گل انگشتانه تهیه گردید. سپس، رده سلولی سرطانی Hela با غلظت‌های مختلف عصاره (125/3، 25/6، 5/12، 25، 50 و 100 میکرگرم بر میلیلیتر) به مدت 24 ساعت تیمار شد و سمیّت سلولی عصاره با روشMTT ارزیابی گردید. در نهایت، میزان بیان ژنهایBax ، Bcl2، P53 وCDH1 در غلظت 50 درصد کشندگی با روشPCR Time-Real مورد بررسی قرار گرفت. داده‌ها با استفاده از نرم‌افزارSPSS و آزمون واریانس یک‌طرفه، تجزیه و تحلیل شدند.یافته‌ها: با توجه به نتایج تست MTT عصاره هیدروالکلی گل انگشتانه دارای اثر سمیّت سلولی وابسته به دوز علیه رده سلولی می‌‌باشد و غلظت 50 درصد کشندگی67/24 میکروگرم بر میلیلیتر تعیین شد. همچنین در غلظت (67/24 میکروگرم بر میلیلیتر) میزان بیان ژنهای آپوپتوزیBax ،CDH1 وP53 افزایش یافته و میزان بیان ژن Bcl2 نسبت به ژن رفرنس کاهش نشان داد.نتیجه‌گیری: عصاره گیاه گل انگشتانه دارای اثر سمیت سلولی و القاء آپوپتوز بر سلول‌های سرطانی Hela است.
چکیده انگلیسی:
Objective: Today, with the high prevalence of cancer, the need for drugs with fewer side effects and better therapeutic effects has been noticed by researchers, so that a large percentage of anti-cancer compounds are prepared from natural sources. The purpose of this study is to investigate the anticancer effects of digitalis nervosa hydroalcoholic extract on uterine cancer cell line (Hela).Materials and methods: In this experimental study, the hydroalcoholic extract of the marigold plant was prepared. Then, Hela cancer cell line was treated with different concentrations of the extract (3.125, 6.25, 12.5, 25, 50 and 100 μg/ml) for 24 hours and the cytotoxicity of the extract was evaluated by MTT method. Finally, the expression level of Bax, Bcl2, P53 and CDH1 genes at 50% lethality concentration was investigated by Time-Real PCR method. Data were analyzed using SPSS software and one-way variance test.Findings: According to the results of the MTT test, the hydroalcoholic extract of foxglove has a dose-dependent cytotoxic effect against the cell line, and the concentration of 50% lethality was determined to be 24.67 micrograms per milliliter. Also, at the concentration (24.67 μg/ml), the expression of apoptotic genes Bax, CDH1 and P53 increased and the expression of Bcl2 gene decreased compared to the reference gene.Conclusion: Thimble flower plant extract has cytotoxicity and apoptosis induction effect on Hela cancer cells.
منابع و مأخذ:
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Shandiz SAS, Farasati S, Saeedi B, Baghbani-Arani F, Akbari Asl E, Keshavarz-Pakseresht B & et al. Up regulation of KAI1 gene expression and apoptosis effect of imatinib mesylate in gastric adenocarcinoma (AGS) cell line. Asian Pacific Journal of Tropical Disease. 2016; 6(2): 120-125.
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Maroufi A, Salimi V & Majdi M. Isolation of Δ5-3β-hydroxysteroid dehydrogenase involved in the biosynthetic pathway of cardenolides and its expression level under the influence of salicylic acid and methyl jasmonate elicitors in foxglove (Digitalis nervosa L). Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research. 2017; 25(1): 97-110.
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Seyedalipour B, Pourakbar E & Taravati A. The Cytotoxic Effect of Ethanolic Extract of Pistacia Khinjuk Leaf on Hela and MCF-7 Cancerous Cell Lines. The Journal of Rafsanjan University of Medical Sciences. 2016; 14(11): 939- 952. [in persian]
Tabatabaee M, Ahmadi R & Kassaee M. The Cytotoxic Effects of Hydroalcoholic Extract of Aerial Parts of Scrophularia striata on MCF-7 Cell Line and Evaluation of BAX and KAI1 Genes Expression. Qom University of Medical Sciences Journal. 2018; 12(9):
38-46. [in persian]
Rezaiyan Gharagozlu N, Sadat Shandiz SA & Baghbani-Arani F. Anti-cancer effects of aqueous and alcoholic licorice root extracts on human hepatocarcinoma (HepG2) cell line. Medical Journal of Tabriz University of Medical Sciences. 2018; 40(2): 40-49. [in persian]
Panahi Kokhdan E, Sadeghi H, Ghafoori H, Sadeghi H, Danaei N, Salaminia S & et al. Apoptotic Effect of the Stachys pilifera Bent Plant Extracts on Colorectal Cancer Cell Line (HT- 29). Armaghane danesh Journal. 2019; 24(1): 17-30. [in persian]
Khosravi F, Moshtaghian J & Zarkesh SH.The effect of ethanolic extract of Achilleawilhelmsii on the survival of three cancer cell lines in vitro culture. Journal of Cell & Tissue. 2021; 11(4): 302-311.
Behboodi S, Baghbani-Arani F, Abdalan S & Sadat Shandiz SA. Green engineered biomolecule-capped silver nanoparticles fabricated from Cichorium intybus extract: in vitro assessment on apoptosis properties toward human breast cancer (MCF-7) cells. Biological trace element research. 2019; 187(2): 392-402.
_||_Cohen PA, Jhingran A, Oaknin A &Denny L. Cervical cancer. The Lancet. 2019; 393(10167): 169-182.
Zhang Z, Zhang D, Xiao BB, Zhang R, Bai HH, Dong HY & et al. Primary study on the relationship between high-risk HPV infection and vaginal cervical microbiota. Zhonghua fu Chan ke za zhi. 2018; 53(7): 471-480.
Abdalan S, Baghbani-Arani F & Sadat Shandiz SA. Evaluation of Anticancer Effect of Aqueous and Hydroalcoholic Extracts of Quercusin Fectoria Leaf against Colon Cancer HT29 Cell Line. Journal of Arak University Medcine Science. 2018; 21(4): 48-57.
Shandiz SAS, Farasati S, Saeedi B, Baghbani-Arani F, Akbari Asl E, Keshavarz-Pakseresht B & et al. Up regulation of KAI1 gene expression and apoptosis effect of imatinib mesylate in gastric adenocarcinoma (AGS) cell line. Asian Pacific Journal of Tropical Disease. 2016; 6(2): 120-125.
Vafaeinezhad Z, Kazemi Z, Mirmoeini M, Piroti H, Sadeghian E, Mohammad Ali-Vajari M & et al. Trends in cervical cancer incidence in Iran according to national cancer registry. Journal of Mazandaran University of Medical Sciences. 2018; 28(161): 108-114. [in persian]
Shirjang S, Mansoori B, Asghari S, Duijf PH, Mohammadi A, Gjerstorff M & Baradaran B. MicroRNAs in cancer cell death pathways: Apoptosis and necroptosis. Free Radical Biology and Medicine. 2019;139: 1-15.
Ghaed Amini N, Fazilati M, Habib-Allahi S, Nazem HA & Hejazi SH. Evaluation of the Effect of Moringa Olifera Hydroalcoholic Extract in a Model of B16F10- Induced Mouse Melanoma Cancer. Experimental animal Biology. 2022; 10(3): 99-111.
Aboeepoor S, Dehghani Ashkezari M, Aboee-Mehrizi F, Haghiralsadat BF & Nikoonahad Lotfabadi N. Designing and Characterizing Nano-carriers Containing Nepeta Persica Extract and Their Effect on Bone Cancer. Internal Medicine Today. 2020; 26 (2): 142-155. [in persian]
Sofalian O, Zare N, Latifi S, Hasanpour Reyhani H & Motallebinia S. Evaluation of anticancer effect of Mentha longifolia and Artemisia persica hydro -alcoholic extracts against human gastric cancer cells (AGS).Genetic Engineering and Biosafety Journal. 2021; 9(1): 1-10.
Badgujar SB, Patel VV, Bandivdekar AH & Mahajan RT. Traditional Uses, Phytochemistry and Pharmacology of Ficus Carica: A Review. Pharmaceutical Biology. 2014; 52(11): 1487-1503.
Al-Sheddi ES, Farshori NN, Al-Oqail MM, Al-Massarani SM, Saquib Q, Wahab R & et al. Anticancer potential of green synthesized silver nanoparticles using extract of Nepeta deflersiana against human cervical cancer cells (Hela). Bioinorganic Chemistry and Applications. 2018. DOI: 10.1155/2018/9390784
Maroufi A, Salimi V & Majdi M. Isolation of Δ5-3β-hydroxysteroid dehydrogenase involved in the biosynthetic pathway of cardenolides and its expression level under the influence of salicylic acid and methyl jasmonate elicitors in foxglove (Digitalis nervosa L). Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research. 2017; 25(1): 97-110.
Chegini S, Tafvizi F & Noorbazargan H. Effect of Valeriana Sysimberifolia Extract on VEGF Expression in A549 Cell Line. Journal of Babol University of Medical Sciences. 2020; 22(1): 222-228. [in persian]
Khatami M & Pourseyedi S. Phoenix dactylifera (date palm) pit aqueous extract mediated novel route for synthesis high stable silver nanoparticles with high antifungal and antibacterial activity. IET nanobiotechnology. 2015; 9(4): 184-190.
Javadi F, Asgharei Moghaadm N & Keshtmand Z. Anti-cancer effects of hydroalcoholic extract of Digitalis nervosa in breast cancer (MCF7) cell line. Plant and biothenology of Iran journal. 2022; 11(1): 21-31. [in persian]
Keshtmand Z, Akbaribazm M, Bagheri Y & Oliaei R. The ameliorative effects of Lactobacillus coagulans and Lactobacillus casei probiotics on CCl4‐induced testicular toxicity based on biochemical, histological and molecular analyses in rat. Andrologia. 2021; 53(1): e13908.
Mousavi SM, Montazeri A, Mohagheghi MA, Jarrahi AM, Harirchi I, Najafi M & et al. Breast Cancer in Iran: An Epidemiological Review. Breast Journal. 2007; 13(4):
383-391.
Azami H, Malek-Hosseini S, Mojahed Taghi M, Zareinejad M & Amirghofran Z. Antitumor Activity and Immunomodulatory Effects of Ficus carica Latex. Journal of Shahid Sadoughi University of Medical Sciences. 2021; 28(12): 3288-3299. [in persian]
Ghazy E & Taghi HS. The autophagy-inducing mechanisms of vitexin, cinobufacini, and physalis alkekengi hydroalcoholic extract against breast cancer in vitro and in vivo. Journal of gastrointestinal cancer. 2021: 1-5.
Nasiri Tarzejani E & Nasri S. Cytotoxicity of methanol extracts of Ziziphora tenuior.L on Hela cell line by MTT assay. New Cellular and Molecular Biotechnology Journa. 2020; 10(38): 69-82. [in persian]
Balouchi A, Fazilati M, Habibollahi S, Nazem H & Hejazi S. The effect of hydroalcoholic extract of Moringa oleifera leaves in a mouse model of colon cancer induced by CACO2 cell line. Journal of jiroft university of medical sciences. 2021; 8(1): 574-585. [in persian]
Seyedalipour B, Pourakbar E & Taravati A. The Cytotoxic Effect of Ethanolic Extract of Pistacia Khinjuk Leaf on Hela and MCF-7 Cancerous Cell Lines. The Journal of Rafsanjan University of Medical Sciences. 2016; 14(11): 939- 952. [in persian]
Tabatabaee M, Ahmadi R & Kassaee M. The Cytotoxic Effects of Hydroalcoholic Extract of Aerial Parts of Scrophularia striata on MCF-7 Cell Line and Evaluation of BAX and KAI1 Genes Expression. Qom University of Medical Sciences Journal. 2018; 12(9):
38-46. [in persian]
Rezaiyan Gharagozlu N, Sadat Shandiz SA & Baghbani-Arani F. Anti-cancer effects of aqueous and alcoholic licorice root extracts on human hepatocarcinoma (HepG2) cell line. Medical Journal of Tabriz University of Medical Sciences. 2018; 40(2): 40-49. [in persian]
Panahi Kokhdan E, Sadeghi H, Ghafoori H, Sadeghi H, Danaei N, Salaminia S & et al. Apoptotic Effect of the Stachys pilifera Bent Plant Extracts on Colorectal Cancer Cell Line (HT- 29). Armaghane danesh Journal. 2019; 24(1): 17-30. [in persian]
Khosravi F, Moshtaghian J & Zarkesh SH.The effect of ethanolic extract of Achilleawilhelmsii on the survival of three cancer cell lines in vitro culture. Journal of Cell & Tissue. 2021; 11(4): 302-311.
Behboodi S, Baghbani-Arani F, Abdalan S & Sadat Shandiz SA. Green engineered biomolecule-capped silver nanoparticles fabricated from Cichorium intybus extract: in vitro assessment on apoptosis properties toward human breast cancer (MCF-7) cells. Biological trace element research. 2019; 187(2): 392-402.
