بررسی اثر موضعی ترکیب روغن کتان و عصاره الکلی دارچین در روند التیام زخم تمام ضخامت جراحی در مدل خرگوش

محورهای موضوعی : آسیب شناسی درمانگاهی دامپزشکی

محمد‌رضا فرهپور ¹ , مجید غفوری ²

1 - استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.
2 - دانشجوی گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.

تاریخ دریافت : 1393/07/10 تاریخ پذیرش : 1393/11/18 تاریخ انتشار : 1393/09/01

کلید واژه: التیام زخم, روغن دانه کتان, عصاره دارچین, زخم برشی, خرگوش,

چکیده مقاله :

هدف از این مطالعه، ارزیابی تاثیر موضعی ترکیب روغن دانه کتان و عصاره هیدروالکلی دارچین در بهبود زخم تجربی پوست در خرگوش بود. شصت خرگوش نیوزلندی به‌طور تصادفی به 4 گروه آزموناختصاص داده شد. در هر خرگوش دو زخم پوستی تمام ضخامت برشی در پهلو و در هر طرف از خط وسط پشت ایجاد شد. خرگوش های گروه 1 هیچ درمانی را دریافت نکردند. خرگوش‌های گروه 2 با پماد دارونما، گروه 3 با پماد روغن کتان 2٪ و گروه 4 با پماد حاوی ترکیب روغن کتان 2٪ به علاوه عصاره هیدروالکلی دارچین 3٪ درمان شدند. مساحت سطح زخم (میلی متر مربع) در روز 4، 8، 12، 16 و 20 با استفاده از یک کاغذ شفاف اندازه گیری شد. در روزهای 3، 7، 14 و 21 نمونه ای از بافت محل ترمیم، برای مطالعات بافت شناسی توسط رنگ آمیزی تری کروم ماسون تهیه شد. با توجه به نتایج، پماد حاوی ترکیب روغن کتان 2٪ + عصاره هیدروالکلی دارچین 3٪ سبب کاهش قابل توجهی در اندازه زخم در تمامی روزهای پس از ایجاد زخم در مقایسه با گروه شاهد شد (05/0>p). همچنین، این ترکیب به‌طور معنی داری موجب انتشار فیبروبلاست ها در حیوانات درمان شده در مقایسه با دیگر گروه ها شد (05/0>p). نتایج نشان داد که تجویز همزمان روغن کتان 2% و عصاره هیدروالکلی دارچین 3% اثرات مفیدی بر روند بهبود زخم دارد.

چکیده انگلیسی:

The aim of the current study was to evaluate of wound healing activity of flaxseed oil and its combination with Cinnamon hydroalcoholic extract on excision of wound healing model in rabbit. Sixty New Zealand white rabbits were randomly allocated into 4 experimental groups. Two full-thickness skin excisional wounds were created on thoracolumbar region on each side of the dorsal midline in each rabbit. Rabbits in group 1 did not receive any treatments. In group 2, rabbits received placebo ointment; in group 3, animals were treated with flaxseed oil 2% ointment. In group 4, rabbits received ointment containing a combination of flaxseed 2%+Cinnamon hydroalcoholic extract 3%. Wound area (mm2) was determined on days 4, 8, 12, 16 and 20 using a transparent paper. Tissue samples were collected on days 3, 7, 14 and 20 for histopathological studies with Masson trichrome staining. According to the results, ointment containing a combination of flaxseed oil 2%+Cinnamon hydroalcoholic extract 3% significantly decreased wound area on all days after wounding in comporison with the control (p<0.05). Also, this combination ointment caused a significantly improved fibroblast distribution in treated animals compared to other groups (p<0.05). Our data showed that co-administration of flaxseed oil 2%+Cinnamon hydroalcoholic extract 3% ointment had beneficial effects on wound healing.

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