بررسی اثر موضعی ترکیب روغن کتان و عصاره الکلی دارچین در روند التیام زخم تمام ضخامت جراحی در مدل خرگوش

محورهای موضوعی : آسیب شناسی درمانگاهی دامپزشکی

محمد‌رضا فرهپور ¹ (استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.)
مجید غفوری ² (دانشجوی گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.)

تاریخ دریافت : 1393/07/10 تاریخ پذیرش : 1393/11/18 تاریخ انتشار : 1393/09/01

کلید واژه: التیام زخم, روغن دانه کتان, عصاره دارچین, زخم برشی, خرگوش,

چکیده مقاله :

هدف از این مطالعه، ارزیابی تاثیر موضعی ترکیب روغن دانه کتان و عصاره هیدروالکلی دارچین در بهبود زخم تجربی پوست در خرگوش بود. شصت خرگوش نیوزلندی به‌طور تصادفی به 4 گروه آزموناختصاص داده شد. در هر خرگوش دو زخم پوستی تمام ضخامت برشی در پهلو و در هر طرف از خط وسط پشت ایجاد شد. خرگوش های گروه 1 هیچ درمانی را دریافت نکردند. خرگوش‌های گروه 2 با پماد دارونما، گروه 3 با پماد روغن کتان 2٪ و گروه 4 با پماد حاوی ترکیب روغن کتان 2٪ به علاوه عصاره هیدروالکلی دارچین 3٪ درمان شدند. مساحت سطح زخم (میلی متر مربع) در روز 4، 8، 12، 16 و 20 با استفاده از یک کاغذ شفاف اندازه گیری شد. در روزهای 3، 7، 14 و 21 نمونه ای از بافت محل ترمیم، برای مطالعات بافت شناسی توسط رنگ آمیزی تری کروم ماسون تهیه شد. با توجه به نتایج، پماد حاوی ترکیب روغن کتان 2٪ + عصاره هیدروالکلی دارچین 3٪ سبب کاهش قابل توجهی در اندازه زخم در تمامی روزهای پس از ایجاد زخم در مقایسه با گروه شاهد شد (05/0>p). همچنین، این ترکیب به‌طور معنی داری موجب انتشار فیبروبلاست ها در حیوانات درمان شده در مقایسه با دیگر گروه ها شد (05/0>p). نتایج نشان داد که تجویز همزمان روغن کتان 2% و عصاره هیدروالکلی دارچین 3% اثرات مفیدی بر روند بهبود زخم دارد.

چکیده انگلیسی:

The aim of the current study was to evaluate of wound healing activity of flaxseed oil and its combination with Cinnamon hydroalcoholic extract on excision of wound healing model in rabbit. Sixty New Zealand white rabbits were randomly allocated into 4 experimental groups. Two full-thickness skin excisional wounds were created on thoracolumbar region on each side of the dorsal midline in each rabbit. Rabbits in group 1 did not receive any treatments. In group 2, rabbits received placebo ointment; in group 3, animals were treated with flaxseed oil 2% ointment. In group 4, rabbits received ointment containing a combination of flaxseed 2%+Cinnamon hydroalcoholic extract 3%. Wound area (mm2) was determined on days 4, 8, 12, 16 and 20 using a transparent paper. Tissue samples were collected on days 3, 7, 14 and 20 for histopathological studies with Masson trichrome staining. According to the results, ointment containing a combination of flaxseed oil 2%+Cinnamon hydroalcoholic extract 3% significantly decreased wound area on all days after wounding in comporison with the control (p<0.05). Also, this combination ointment caused a significantly improved fibroblast distribution in treated animals compared to other groups (p<0.05). Our data showed that co-administration of flaxseed oil 2%+Cinnamon hydroalcoholic extract 3% ointment had beneficial effects on wound healing.

منابع و مأخذ:

• Amara, A.A., El-Masry, M.H. and Bogdady, H.H. (2008). Plant crude extracts could be the solution: Extracts showing in vivo antitumorigenic activity. Pakistan Journal of Pharmaceutical Sciences, 21: 159-171.


• Asadpour, R., Azari, M., Hejazi, M., Tayefi, H. and Zaboli, N. (2013). Protective effects of garlic aquous extract (Allium sativum), vitamin E, and N-acetylcysteine on reproductive quality of male rats exposed to lead. Veterinary Research Forum, 4(4): 251-257.


• Binic, I., Lazarevic, V., Ljubenovic, M., Mojsa, J. and Sokolovic, D. (2013). Skin Ageing: Natural Weapons and Strategies. Evidence-Based Complementary and Alternative Medicine, 827248.


• Boelsma, E., Hendriks, H.F.J. and Roza, L. (2001). Nutritional skin care: health effects of micronutrients and fatty acids. The American Journal of Clinical Nutrition, 73: 853-864.


• Chen, W., Liou, S., Tzeng, T., Lee, S. and Liu, I. (2012). Wound repair and anti-inflammatory potential o Lonicera japonica in excision wound-induced rats. BMC Complementary and Alternative Medicine, 12(226): 2-9.


• Czemplik, M.,  Kulma, A.,  Bazela, K. and Szopa, J. (2012). The biomedical potential of genetically modified flax seeds overexpressing the glucosyltransferase gene. BMC Complementary and Alternative Medicine, 12: 251.  


• Estevão, L.R.M., de Souza Mendonça, F., Baratella-Evêncio, L., Simões, R.S., de Barros, M.E. G., Arantes, R.M.E., et al. (2013). Effects of aroeira (Schinus terebinthifoliu Raddi) oil on cutaneous wound healing in rats. Acta Cirúrgica Brasileira, 28(3): 202-209.


• Eseyin, O.A., Ebong, P., Ekpo, A., Igboasoiyi, A. and Oforah,  E. (2007).  Hypoglycemic  effect  of  the  seed extract  of  Telfairia  occidentalis  in  rat.  Pakistan Journal of Biological Sciences, 10(3): 498-501.


• Farahpour, M.R. and Habibi, M. (2012). Evaluation of the wound healing activity of an ethanolic extract of Ceylon cinnamon in mice. Veterinarni Medicina, 57(1): 53-57.


• Farahpour, M.R., Taghikhani, H., Habibi, M. and Zandieh, M.A. (2011). Wound healing activity of flaxseed Linum usitatissimum L. in rats. African Journal of Pharmacy and Pharmacology, 5(21): 2386-2389.


• Jianming., L., Keqiang., Z., Sangkil., N., Richard., A., Anderson., R.J. and Wei, W. (2010). Novel angiogenesis inhibitory activity in cinnamon extract blocks VEGFR2 kinase and downstream signaling. Carcinogenesis, 31(3): 481-488.


• Koch, A.E., Polverini, P.J., Kunkel, S.L., Harlow, L.A., DiPietro, L.A., Elner, V.M., et al. (1992). Interleukin-8 as a Macrophage-Derived Mediator of Angiogenesis. Science, 258: 1789-1800.


• McDaniel, J.C., Massey, K. and Nicolaou, A. (2011). Fish oil supplementation alters levels of lipid mediators of inflammation in microenvironment of acute human wounds. Wound Repair Regen, 19(2): 189-200.


• Naeini, A.T., Oryan, A., Dehghani, S. and Nikahval, B. (2008). Experimental cutaneous wound healing in rabbits: using continuous microamperage low-voltage electrical stimulation. Comprative Clinical Pathology, 17: 203-210.


• Nesbitt, P.D. and Thompson, L.U. (1997). Lignans in homemade and commercial products containing flaxseed. Nutrition and Cancer, 29(3): 222-227.


• Otranto, M., Nascimento, A.P. and Monte-Alto-Costa, A. (2010). Effects  of  supplementation  with  different  edible  oils  on cutaneous wound healing. Wound Repair and Regeneration, 18(6): 629-636.


• Oussalah, M., Caillet, S. and Lacroix, M. (2006). Mechanism of action of Spanish oregano, Chinese cinnamon, and savory essential oils against cell membranes and walls of Escherichia coli O157:H7 and Listeria monocytogenes. Journal of Food P otection, 69(5): 1046-1055. 


• Purohit, S.K., Solanki, R., Soni, M.K. and Mathur, V. (2012). Experimental evaluation of aloe vera leaves pulp as topical medicament on wound healing. Intern Journal Pharmacology Research, 2(3): 110-112.


• Qin, B., Nagasaki, M., Ren, M., Bajotto, G., Oshida, Y. and Sato, Y. (2003). Cinnamon extract (traditional herb) potentiates in vivo insulin-regulated glucose utilization via enhancing insulin signaling in rats. Diabetes Research Clinic Practice, 62(3): 139-148. 


• Sharifi, R., Rastegar, H., Kamalinejad, M., Dehpour, A.R., Tavangar, S.M., Paknejad, et al.  (2012). Effect of Topical Application of Silymarin (Silybum marianum) on Excision Wound Healing in Albino Rats. Acta Medica Iranica, 50(9): 583-588.


• Singh, G., Maurya, S., DeLampasona, M.P. and Catalan, C.A. (2007). A comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents. Food and Chemical Toxicology, 45(9): 1650-1661.


• Süntar, I., Akkol, E.K., Keleş, H., Oktem, A., Başer, K.H. and Yeşilada, E. (2011). A novel wound healing ointment: a formulation of Hypericum perforatum oil and sage and oregano essential oils based on traditional Turkish knowledge. Journal of Ethnopharmacology, 134(1): 89- 96.


• Thomas, A., Wynn, K. and Luke, B. (2010). Macrophages: Master Regulators of Inflammation and Fibrosis. Seminars in Liver Disease, 30(3): 245-257.


• Trivellato Grassi, L., Malheiros, A., Meyre-Silva, C., da Silva Buss, Z., Monguilhott, E.D., Fröde, T.S., et al. (2013). From popular use to pharmacological validation: A study of the anti-inflammatory, anti-nociceptive and healing effects of Chenopodium ambrosioides extract. Journal of  Ethnopharmacology, 145(1): 127-138.


• Tumen, I., Suntar, I., Keles, H. and Akkol, E.K. (2012). A Therapeutic Approach forWound Healing by Using Essential Oils of Cupressus and Juniperus Species Growing in Turkey. Evidence-Based Complementary and Alternative Medicine, 728281.


• Walker, H.L. and Mason, J.A.D. (1968). A standard animal burn. Journal of Trauma-Injury, Infection Critical Care, 8(6): 1049-1051.