ارزیابی وضعیت تعادل اکسیدانی-آنتی‌اکسیدانی پلاسما با افزودن عصاره آبی پیاز گیاه سیر (Allium sativum) به شیر بزغاله‌های شیرخوار
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
مصطفی عبداللهی
1
,
اشکان جبلی جوان
2
1 - دستیار تخصصی بیماری‌های داخلی دام‌های بزرگ، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران.
2 - دانشیار گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه سمنان، سمنان، ایران.
تاریخ دریافت : 1398/04/28
تاریخ پذیرش : 1398/11/26
تاریخ انتشار : 1398/11/01
کلید واژه:
آنتی‌اکسیدان,
سیر,
عصاره آبی,
اکسیدان,
بزغاله شیرخوار,
چکیده مقاله :
در نوزاد نشخوارکنندگان به علت نابالغ بودن سیستم ایمنی، نسبت به عفونت ها حساسیت بالایی وجود دارد. آنتی اکسیدان ها قادر به تقویت عملکرد سلول های سیستم ایمنی می باشند و گیاه سیر(Allium sativum) هم آنتی اکسیدانی است که در مورد نوزاد نشخوارکنندگان به عنوان یک آنتی بیوتیک پروفیلاکتیک مطرح شده است. مطالعه حاضر با هدف تعیین وضعیت تعادل اکسیدانی-آنتی‌اکسیدانی پلاسما دراثر افزودن عصاره آبی پیاز گیاه سیر به شیر بزغاله های نوزاد انجام شد. این مطالعه روی 30 رأس بزغاله نوزاد نر و ماده 7 روزه نژاد مخلوط شیرخوار با میانگین وزن 3 کیلوگرم انجام گرفت. بزغاله ها به 3 گروه مساوی تقسیم شدند. هر گروه یکی از 3 درمان خوراکی شامل 30 میلی لیتر سالین (درمان کنترل منفی)، 400 واحد ویتامین E و 83 میلی گرم بر کیلوگرم وزن بدن عصاره آبی سیر را در هر 3 وعده دریافت شیر در طول 24 ساعت، دریافت کردند. در دقیقه صفر و نیز ساعات 1، 5/2، 5، 12، 18 و 24 پس از شروع درمان، جداگانه نمونه خون از همه دام های مورد آزمایش اخذ و پس از جداسازی پلاسمای هر نمونه،میزان ظرفیت هایآنتی اکسیدانی و اکسیدانی تام پلاسما و اندیس استرس اکسیداتیو آن تعیین شد. یافته ها نشان داد که در مقایسه با درمان کنترل منفی، درمان با ویتامین E سبب سوق معنی دار تعادل اکسیدانی-آنتی اکسیدانی پلاسما به سمت آنتی اکسیدانی (05/0>p ) و درمان با سیر سبب سوق تعادل مذکور به سمت اکسیدانی (05/0> p ) گردیده و هیچ نوع عارضه جانبی بالینی هم به دنبال تجویز ترکیبات مذکور در بزغاله ها مشاهده نشد. این مطالعه نشان داد که عصاره آبی سیر دارای اثر اکسیدانی در پلاسمای بزغاله های نوزاد شیرخوار می باشد.
چکیده انگلیسی:
The ruminant infant is susceptible to neonatal infections due to the immature immune system. Antioxidants can enhance the function of the immune system cells. Garlic is an antioxidant that has been proposed as a prophylactic antibiotic in ruminant infants. The aim of this study was to determine the effect of adding aqueous extract of garlic to milk on the plasma oxidant-antioxidant balance in neonatal goat kids. This study was performed on 30 male and female suckling 7-day-old neonatal goat kids of mixed breed (mean weight 3 kg). The goat kids were divided into 3 equal groups. Each group received one of the following three oral treatments (inclusive 30 ml of saline, 400 IU of vitamin E, 83 mg / kg of aqueous extract of garlic) at every 3 milk meals per 24 hours. At 0 min and 1, 2/5, 5, 12, 18, and 24 hours after the beginning of the first treatment, blood samples were taken and the plasma was discarded and the total antioxidant and total oxidation capacity of the plasma and its oxidative stress index were determined. Compared to negative control, treatment with vitamin E caused a significant tendency of the oxidant-antioxidant balance of the plasma towards the antioxidant (p < /p>
منابع و مأخذ:
Abdollahi, M., Mohammadi H.R., Ahmadi-Hamedani, M., Abdollahi, M. and Shahbazi, V. (2019). The effect of oral administration of Folus (Cassia fistula) aqueous extract on abomasal emptying rate in neonatal lambs. Journal of Veterinary Clinical Pathology, 13(49): 67-75. [In Persian]
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De-La-Fuente, M. (2002). Effects of antioxidants on immune system ageing. European Journal of Clinical Nutrition, 56(3): 55-58.
Dokuyucu, R., Karateke, A., Gokce, H., Kurt, R.K., Ozcan, O. and Ozturk, S. (2014). Antioxidant effect of erdosteine and lipoic acid in ovarian ischemia-reperfusion injury. European Journal of Obstetrics & Gynecology and Reproductive Biology, 183(4): 23-27.
Donovan, D.C., Frenklin, S.T., Chase, C.C.L. and Hippen, A.R. (2002). Growth and health of Holstein calves fed milk replacers supplemented with antibiotics or enteroguard. Journal of Dairy Science, 85(4): 947-950.
El-Sayed, Y.S., El-Okle, O.S.M., Hassan, S.M.H. and Bakir, N.M.A. (2015). Poisoning of Cattle Feeding on Allium ampeloprasum (Egyptian kurrat). Journal of Veterinary Science & Medical Diagnosis, 4(4): 1-4.
Erel, O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry, 37(4): 277-285.
Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12): 1103-1111.
Esmaeili, S., Vaez, E., Yassini, A., Mortazavian, A.M., Sohrabvandi, S. and Ferdosi, R. (2016). The effect of inulin on the qualitative characteristics of malt beverage during storage at different temperatures. Iranian Journal of Nutrition Sciences and Food Technology, 11(1): 113-120. [In Persian]
Fernandez, A., Ramos, J.J., Loste, A., Ferrer L.M., Figueras, L., Verde, M.T., et al. (2006). Influence of colostrum treated by heat on immunity function in goat kids. Comparative Immunology, Microbiology & Infectious Diseases, 29(5): 353-364.
Ghosh, S., Mehla, R.K., Sirohi, S.K. and Tomar, S.K. (2011). Performance on crossbred calves with dietary supplementation of garlic extract. Journal of Animal Physiology and Animal Nutrition, 95(4): 449-455.
Javanmardi, J., Stushnoff, C., Locke, E. and Vivanco, J.M. (2003). Antioxidant activity and total phenolic content of Iranian ocimum accessions. Food Chemistry, 83(4): 547-550.
Khodadad, S., Mohajeri, D. and Kaffashi Elahi, R. (2018). The effect of hydroalcoholic extract of Brasscia rapa. L root on methotrexate induced hepatotoxicity in the rat. Journal of Veterinary Clinical Pathology, 12(46): 167-191. [In Persian]
Maritim, A.C., Sanders, R.A. and Watkins, J.B. (2003). Diabetes, oxidative stress, and antioxidants: a review. Journal of Biochemical and Molecular Toxicology, 17(1): 24-38.
Mohammadzadeh, H., Delashoub, M. and Khakpour, M. (2018). Effect of vitamin E in prevention of lipopolysaccharide induced fetal injuries in the rat. Journal of Veterinary Clinical Pathology, 11(44): 367-395. [In Persian]
Mokhber-Dezfouli, M.R., Mohammadi, H.R., Nadalian, M.Gh., Nazem-Bokaee, Z., Hadjiakhoondi, A. and Nikbakht-Borujeni, Gh,R. (2011). Influence of parenteral administration of chamomile (Matricaria recutita L.) extract on colostral IgG absorption in neonatal calves. Iran Journal of Veterinary Medicine, 5(3): 169-171. [In Persian]
Rivlin, R.S. (2001). Historical perspective on the Use of Garlic. The Journal of Nutrition, 131(3): 951-954.
Rodriguez, C., Castro, N., Capote, J., Morales-delaNuez, A., Moreno-Indias, I., Sanchez-Macias, D. and Arguello, A. (2009). Effect of colostrums immunoglobulin concentration on immunity in Majorera goat kids. Journal of Dairy Science, 92(4): 1696-1701.
Salimnejad, R., Jalali, M., Nikravesh, M.R. and Fazel, A.R. (2014). Effect of garlic aqueous extract on markers of oxidative stress in diabetic rats testes. Journal of Rafsanjan University of Medical Sciences, 13(4): 371-382. [In Persian]
Sargison, N., Crilly, J.P. and Hopker, A. (2018). Practical lambing and lamb care- A Veterinary Guide. 4th ed. John Wiley and Sons Ltd. UK, Oxford, pp: 44.
Schaer, S., Herrli‐Gygi, M., Kosmeas, N., Boschung, H. and Steiner, A. (2005). Characteristics of acetaminophen absorption in healthy unweaned calves as an indirect measurement of the oroduodenal transit rate of liquid meals. Transboundary and Emerging Diseases, 52(7): 325-332.
Shokrollahi, B., Hesami, S.M. and Baneh, H. (2016). The effect of garlic extract on growth, haematology and cell-mediated immune response of newborn goat kids. Journal of Agriculture and Rural Development in the Tropics and Subtropics, 117(2): 225-232.
Taji, F., Shirzad, H., Ashrafi, K., Parvin, N., Kheiri, S. and Namjoo, A. (2012). A comparison between the antioxidant strength of the fresh and stale Allium sativum (garlic) extracts. Zahedan Journal of Research in Medical Sciences, 14(5): 25-9. [In Persian]
Zhu, Y., Anand, R., Geng, X. and Ding, Y. (2018). A mini review: garlic extract and vascular diseases. Neurological Research, 40(6): 421-425.
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Abdollahi, M., Mohammadi H.R., Ahmadi-Hamedani, M., Abdollahi, M. and Shahbazi, V. (2019). The effect of oral administration of Folus (Cassia fistula) aqueous extract on abomasal emptying rate in neonatal lambs. Journal of Veterinary Clinical Pathology, 13(49): 67-75. [In Persian]
Ankri, S. and Mirelman, D. (1999). Antimicrobial properties of allicin from garlic. Microbes and Infection, 1(2): 125-129.
Bakri, I.M. and Douglas, C.W.I. (2005). Inhibitory effect of garlic on oral bacteria. Archives of Oral Biology, 50(7): 645-651.
Borek, C. (2001). Antioxidant health effects of aged garlic extract. The Journal of Nutrition, 131(3): 1010-1015.
Dalal, J.H., Macneil, J.H. and Yared, D.M. (1987). Antioxidant activity of onion and garlic juices in stored cooked ground lamb. Journal of Food Protection, 50(5): 411-413.
Daniels, J.T., Hatfield, P.G., Burgess, D.E., Kottt, R.W. and Bowman, J.G. (2000). Evaluation of ewe and lamb immune response when ewes were supplemented with vitamin E. Journal of Animal Science, 78(10): 2731-2736.
De-La-Fuente, M. (2002). Effects of antioxidants on immune system ageing. European Journal of Clinical Nutrition, 56(3): 55-58.
Dokuyucu, R., Karateke, A., Gokce, H., Kurt, R.K., Ozcan, O. and Ozturk, S. (2014). Antioxidant effect of erdosteine and lipoic acid in ovarian ischemia-reperfusion injury. European Journal of Obstetrics & Gynecology and Reproductive Biology, 183(4): 23-27.
Donovan, D.C., Frenklin, S.T., Chase, C.C.L. and Hippen, A.R. (2002). Growth and health of Holstein calves fed milk replacers supplemented with antibiotics or enteroguard. Journal of Dairy Science, 85(4): 947-950.
El-Sayed, Y.S., El-Okle, O.S.M., Hassan, S.M.H. and Bakir, N.M.A. (2015). Poisoning of Cattle Feeding on Allium ampeloprasum (Egyptian kurrat). Journal of Veterinary Science & Medical Diagnosis, 4(4): 1-4.
Erel, O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry, 37(4): 277-285.
Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12): 1103-1111.
Esmaeili, S., Vaez, E., Yassini, A., Mortazavian, A.M., Sohrabvandi, S. and Ferdosi, R. (2016). The effect of inulin on the qualitative characteristics of malt beverage during storage at different temperatures. Iranian Journal of Nutrition Sciences and Food Technology, 11(1): 113-120. [In Persian]
Fernandez, A., Ramos, J.J., Loste, A., Ferrer L.M., Figueras, L., Verde, M.T., et al. (2006). Influence of colostrum treated by heat on immunity function in goat kids. Comparative Immunology, Microbiology & Infectious Diseases, 29(5): 353-364.
Ghosh, S., Mehla, R.K., Sirohi, S.K. and Tomar, S.K. (2011). Performance on crossbred calves with dietary supplementation of garlic extract. Journal of Animal Physiology and Animal Nutrition, 95(4): 449-455.
Javanmardi, J., Stushnoff, C., Locke, E. and Vivanco, J.M. (2003). Antioxidant activity and total phenolic content of Iranian ocimum accessions. Food Chemistry, 83(4): 547-550.
Khodadad, S., Mohajeri, D. and Kaffashi Elahi, R. (2018). The effect of hydroalcoholic extract of Brasscia rapa. L root on methotrexate induced hepatotoxicity in the rat. Journal of Veterinary Clinical Pathology, 12(46): 167-191. [In Persian]
Maritim, A.C., Sanders, R.A. and Watkins, J.B. (2003). Diabetes, oxidative stress, and antioxidants: a review. Journal of Biochemical and Molecular Toxicology, 17(1): 24-38.
Mohammadzadeh, H., Delashoub, M. and Khakpour, M. (2018). Effect of vitamin E in prevention of lipopolysaccharide induced fetal injuries in the rat. Journal of Veterinary Clinical Pathology, 11(44): 367-395. [In Persian]
Mokhber-Dezfouli, M.R., Mohammadi, H.R., Nadalian, M.Gh., Nazem-Bokaee, Z., Hadjiakhoondi, A. and Nikbakht-Borujeni, Gh,R. (2011). Influence of parenteral administration of chamomile (Matricaria recutita L.) extract on colostral IgG absorption in neonatal calves. Iran Journal of Veterinary Medicine, 5(3): 169-171. [In Persian]
Rivlin, R.S. (2001). Historical perspective on the Use of Garlic. The Journal of Nutrition, 131(3): 951-954.
Rodriguez, C., Castro, N., Capote, J., Morales-delaNuez, A., Moreno-Indias, I., Sanchez-Macias, D. and Arguello, A. (2009). Effect of colostrums immunoglobulin concentration on immunity in Majorera goat kids. Journal of Dairy Science, 92(4): 1696-1701.
Salimnejad, R., Jalali, M., Nikravesh, M.R. and Fazel, A.R. (2014). Effect of garlic aqueous extract on markers of oxidative stress in diabetic rats testes. Journal of Rafsanjan University of Medical Sciences, 13(4): 371-382. [In Persian]
Sargison, N., Crilly, J.P. and Hopker, A. (2018). Practical lambing and lamb care- A Veterinary Guide. 4th ed. John Wiley and Sons Ltd. UK, Oxford, pp: 44.
Schaer, S., Herrli‐Gygi, M., Kosmeas, N., Boschung, H. and Steiner, A. (2005). Characteristics of acetaminophen absorption in healthy unweaned calves as an indirect measurement of the oroduodenal transit rate of liquid meals. Transboundary and Emerging Diseases, 52(7): 325-332.
Shokrollahi, B., Hesami, S.M. and Baneh, H. (2016). The effect of garlic extract on growth, haematology and cell-mediated immune response of newborn goat kids. Journal of Agriculture and Rural Development in the Tropics and Subtropics, 117(2): 225-232.
Taji, F., Shirzad, H., Ashrafi, K., Parvin, N., Kheiri, S. and Namjoo, A. (2012). A comparison between the antioxidant strength of the fresh and stale Allium sativum (garlic) extracts. Zahedan Journal of Research in Medical Sciences, 14(5): 25-9. [In Persian]
Zhu, Y., Anand, R., Geng, X. and Ding, Y. (2018). A mini review: garlic extract and vascular diseases. Neurological Research, 40(6): 421-425.
