Plantlet Regeneration through Indirect Organogenesis of Flame Gold Tree (Koelreuteria elegans Laxm.)

محورهای موضوعی : مجله گیاهان زینتی

Rameshwar Groach ¹ , Muzafar Hussain Dar ² , Kartar Chand Badgal ³ , Priyanka Pal ⁴ , Narender Singh ⁵ , Kuldeep Yadav ⁶

1 - Department of Botany, Kurukshetra University, Kurukshetra 136119 (India)
2 - Department of Botany, Kurukshetra University, Kurukshetra 136119 (India)
3 - Principal, Govt. Degree College, Hiranagar, Kathua
4 - Department of Botany, Kurukshetra University, Kurukshetra 136119 (India)
5 - Department of Botany, Kurukshetra University, Kurukshetra 136119 (India)
6 - Department of Botany, Kurukshetra University, Kurukshetra 136119 (India)

تاریخ دریافت : 1394/05/17 تاریخ پذیرش : 1394/05/17 تاریخ انتشار : 1393/06/10

کلید واژه: Auxins, Cytokinins, Koelreuteria elegans, Ornamental tree,

چکیده مقاله :

Koelreuteria elegans, popularly known as “Flame Gold” is an ornamental tree. In vitro callus induction and regeneration from various explants (eaf segments and cotyledonary leaf) were studied on modified MS medium. The highest callus induction rate (80%) and multiplication was obtained in 2 mg/l 2,4-D from leaf segments. Calli transferred in 1.5 mg/l BAP resulted in efficient shoot regeneration (70%) and development (4.35 shoots). MS half strength medium supplemented with 0.2 mg/l NAA reported 80% rooting after 21 days of implantation. Mostly, the roots were long and healthy. Plants were successfully transferred in sterilized mixture of vermiculite: soil: sand (3:1:1) with 65% survival rate under field conditions. The in vitro regenerated plantlets were hardened and acclimatized successfully.

چکیده انگلیسی:

Koelreuteria elegans, popularly known as “Flame Gold” is an ornamental tree. In vitro callus induction and regeneration from various explants (eaf segments and cotyledonary leaf) were studied on modified MS medium. The highest callus induction rate (80%) and multiplication was obtained in 2 mg/l 2,4-D from leaf segments. Calli transferred in 1.5 mg/l BAP resulted in efficient shoot regeneration (70%) and development (4.35 shoots). MS half strength medium supplemented with 0.2 mg/l NAA reported 80% rooting after 21 days of implantation. Mostly, the roots were long and healthy. Plants were successfully transferred in sterilized mixture of vermiculite: soil: sand (3:1:1) with 65% survival rate under field conditions. The in vitro regenerated plantlets were hardened and acclimatized successfully.

منابع و مأخذ:

1. Agarwal M., and Kamal, R. 2004. In vitro colonal propagation of Momordica charantia L. Indian Journal of Biotechnology, 3: 426-430.

2. Atta-Alla, H. and Van Staden, J. 1997. Micropropagation and establishment of Yucca aloifolia. Plant Cell Tissue Organ Culture;48(3):209-212. [DOI via Crossref]

3. Feng, D.L., Zhang, J., Liu, X., Peng, W.X. and Wu, T.Y. 2009. In vitro calture of immature embryo from Koelreuteria bipinnata var ̒integrifolia̓. Foresty Studies in China, 11(3): 179-184. [DOI via Crossref]

4. Getu, T., and Feyissa, T. 2013. In vitro regeneration of sweet potato (Ipomoea batatas (L.) Lam.) Convolvulaceae, from leaf and petiole explants. Ethiopian Journal of Biological Sciences, 11: 147-162.

5. Gilman, E.F., and Watson, D. G. 1993. Koelreuteria elegans Flame Gold. U.S. Forest Service Fact Sheet ST-337. Available http://hort.ifas.ufl.edu/database/documents/pdf/tree_fact_sheets/koeelea.pdf (viewed 8-28-2014).

6. Kumari, S., and Singh, N. 2012. In vitro plantlet regeneration from cotyledonary node explants of Salvadora persica L. a medicinally important desert tree. Journal of Agricultural Technology, 8: 1839-1854.

7. Lal, D., and Singh, N., Yadav K. 2010. In vitro studies on Celastrus paniculatus. Journal of Tropical Medicinal Plants, 11: 169-174.

8. Lavanya, A.R., Muthukrishnan, S., Kumaresan, V., Franklin Benjamin, J.H., and Rao, M.V. 2012. In vitro micropropagation of Hildegardia populifolia (Roxb.) Schott & Endl., an endangered tree species from Eastern Ghats of Tamil Nadu. Indian Journal of Agricultural Technology, 8: 1727-1744.

9. Mithila, J., Hall, J., Victor, J.M.R. and Saxena, P.K. 2003. Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionanthaWendl.). Plant Cell Reports;21(5):408-414. [Pubmed]

10. Meyer, F. G. 1976. A revision of the genus Koelreuteria (Sapindaceae). Journal of the Arnold Arboretum, 57: 129–166.

11. Murashige, T., and Skoog, F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15: 473-495. [DOI via Crossref]

12. Nagaraju, S., Reddy, S.K. and Farook, S.A. 1998. Propagation of Ficus reliosa L. from maxillary buds and shoot tips. Adv Plant Science;11(2):287-290.

13. Osternack, N., Saare-Surminski, K., Preil, W. and Lieberei, R. 1999. Induction of somatic embryos, adventitious shoots and roots in hypocotylstissue of Euphorbia pulcherrimaWilld. Ex Klotzsch: comparative studies on embryogenic and organogenic competence. Journal of Applied Botany; 73:197-201.

14. Rawat, J. M., Rawat, B., Chandra, A., and Nautiyal, S. 2013. Influence of plant growth regulators on indirect shoot organogenesis and secondary metabolite production in Aconitum violaceum Jacq. African Journal of Biotechnology, 12: 6287-6293.

15. Sharma, T., Khan, M. K., Misra, P., and Shukla, P. K. 2012. Micropropagation of kinnow through nodal explants. The Biosean, 7: 295-297.

16. Sita, G. L., and Rani, B. S. 1985. In vitro propagation of Eucalyptus grandis L. by tissue culture. Plant Cell Reports, 4: 63-65. [DOI via Crossref]    [Pubmed]

17. Teware, K., Singh, P., and Mehta, R. 2012. In vitro callus induction from stem explants of Cissus quadrangularis L. (Hadjod). International Journal of Ayurvedic and Herbal Medicine 2: 135-138.

18. Van der Salm, T.P.M., Van der Toorn, C.J.G., Hanisch-ten Cate, C.H. and Dons, H.J.M. 1996. Somatic embryogenesis and shoot regeneration from excised adventitious roots of the root stock Rosa hybrida cv. ̒Money Way̓. Plant Cell Reports;15:522-526. [DOI via Crossref]

19. Yadav, K., and Singh, N. 2010. Micropropagation of Spilanthes acmella Murr. an important medicinal plant. Nature and Science, 8: 5-11.

20. Yadav, K., and Singh, N. 2011. In vitro propagation and biochemical analysis of field established wood apple (Aegle marmelos L.). Analele Universităţii din Oradea Fascicula Biologie, 18: 23-28.

21. Yadav, K., Aggarwal, A. and Singh, N. 2013. Evaluation of genetic fidelity among micropropagated plants of Gloriosa superba L. using DNA-based markers—a potential medicinal plant. Fitoterapia, 89:265-270. [DOI via Crossref]    [Pubmed]

22. Zakizadeh, S., Kaviani, B., and Onsinejad, R. 2013. In vitro rooting of amaryllis (Hippeastrum johnsonii), a bulbous plant, via NAA and 2-iP. Annals of Biological Research, 4: 69-71.