The Effects of Naphthaleneacetic Acid on the Rooting of Cycas revoluta Bulbs

محورهای موضوعی : مجله گیاهان زینتی

Mehdi Asgari Gouraj ¹

1 - Landscape Group, Department of Agriculture, Chalus Branch, Islamic Azad University, Chalus, Iran

تاریخ دریافت : 1402/01/31 تاریخ پذیرش : 1402/05/23 تاریخ انتشار : 1402/06/10

کلید واژه: Chlorophyll, root length, Leaf fresh weight, Naphthaleneacetic acid, <i>Cycas revoluta</i>,

چکیده مقاله :

The effects of naphthaleneacetic acid (NAA) (0, 15, 20 and 25 mg/l) were explored on the rooting of Cycas revoluta bulbs in a study based on a randomized complete block design with four replications. Based on the results, the NAA treatments significantly influenced root length, leaf fresh and dry weight, and chlorophyll. But, they did not affect root number, root fresh and dry weight, significantly. The longest roots were 9.43 and 9.63 cm produced by the bulbs treated with 25 and 20 mg/l NAA, respectively. The 25 and 20 mg/l NAA were related to the highest root number of 3.57 and 3.23, respectively, the 25 mg/l NAA was related to the highest mean root fresh weight of 4.9 g, the 25 mg/l NAA was related to the highest root dry weight of 2.5 g, the 20 and 25 mg/l NAA were related to the highest leaf fresh weight of 6.04 and 6.54 g, respectively, the 25 and 20 mg/l NAA were related to the highest leaf dry weight of 4.07 and 3.11 g, respectively, and the 20 and 5 mg/l NAA were related to the highest chlorophyll content of 6.28 and 6.22 mg g-1 F.W., respectively. A significant relationship was observed between leaf dry weight and chlorophyll content at the P < 0.01 level (+0.78). But, there was no significant relationship between root dry weight and mean root length (+0.29). The relationship between chlorophyll content and mean root length was not significant either (+0.13). According to the results, increasing the hormone rate may even affect the plants adversely, injuring them. In general, it can be inferred from the results that NAA at the rate of 20 mg/l influenced the studied traits, thereby entailing the highest gene expression in C. revoluta.

چکیده انگلیسی:

این تحقیق با هدف بررسی اثر نفتالین استیک اسید (NAA) (15، 10، 0 و 25 میلی‌گرم در لیتر) بر ریشه‌زایی پیاز گیاه سیکاس رولوتا (Cycas revolute) در قالب طرح بلوک‌های کامل تصادفی با چهار تکرار انجام شد. نتایج نشان داد که اثر NAA روی طول ریشه، وزن تر و خشک برگ و کلروفیل معنی‌دار بود اما تاثیر معناداری بر تعداد ریشه، وزن تر و خشک ریشه نداشت. تیمارهای 25 و 20 میلی‌گرم در لیتر NAA به‌ترتیب با 9/43 و 9/63 سانتی‌متر بیشترین طول ریشه را داشتند. بیشترین تعداد ریشه به‌ترتیب با 3/57 و 3/23 ریشه متعلق به تیمارهای 25 و 20 NAA بود. تیمار 25 میلی‌گرم در لیتر NAA بیشترین میانگین وزن تر (9/4 گرم) و وزن خشک ریشه (2/5 گرم) را داشت. تیمارهای 20 و 25 میلی‌گرم در لیتر NAA به‌ترتیب با 6/04 و 6/54 گرم بیشترین وزن تر برگ، 25 و 20 میلی‌گرم در لیتر NAA به‌ترتیب با 4/07 و 3/11 گرم بیشترین وزن خشک برگ و تیمارهای 20 و 25 میلی‌گرم در لیتر NAA به‌ترتیب با 6/28 و 6/22 ‌میلی‌گرم در هر گرم وزن تر بیشترین میزان کلروفیل را داشتند. بین وزن خشک برگ با میزان کلروفیل (0/78+) در سطح یک درصد رابطه معنی‌داری وجود داشت اما میان وزن خشک ریشه با میانگین طول ریشه (0/29+) رابطه معنی‌داری وجود نداشت. بین میزان کلروفیل با میانگین طول ریشه (0/13+) هیچ رابطه معنی‌داری وجود ندارد. نتایج این تحقیق نشان داد که همیشه با افزایش غلظت هورمون، بیان ژن افزایش نیافته و این امکان وجود دارد که افزایش غلظت هورمون، حتی باعث اثر عکس بر روی گیاه و آسیب رسیدن به آن شود. به‌طور کلی نتایج نشان داد مصرف 20 میلی‌گرم در لیتر NAA با تاثیر بر صفات مورد بررسی بیشترین بیان ژن را در گیاه سیکاس داشت.

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