Effect of Hydrogen Peroxide, Ascorbic Acid and Indolic-3-Butyric Acid on Root Induction and Development in Cuttings of Bougainvillea spectabilis
محورهای موضوعی : مجله گیاهان زینتی
Mehdi Pirdastan
1
,
Abdolhossein Aboutalebi Jahromi
2
,
Hamed Hassanzadeh Khankahdani
3
1 - Former M.Sc Student, Horticulture, Jahrom Branch, Islamic Azad University, Jahrom, Iran
2 - Associate Professor of Horticulture, Jahrom Branch, Islamic Azad University, Jahrom, Iran
3 - Researcher of Horticulture Crops Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran
کلید واژه: Rooting, IBA, vitamin C, H2O2, Long-term and quick dipping,
چکیده مقاله :
Bougainvillea (Bougainvilleaspectabilis) is used for planting in the landscape because of their beautiful and colorful flowers. The easiest and cheapest technique to propagate this plant is by cuttings; however the cuttings of this plant are not easily rooted and require the special treatments, including the use of auxin. For this purpose, an experiment was performed in Completely Randomized Design with 15 treatments and four replications and the characteristics such as root and shoot length, root and shoot number, root fresh and dry weight, and the rooted cuttings percent were evaluated. The results showed a statistically significant difference among the used treatments. The use of 400 mg/L indolic-3-butyric acid (IBA) for 24 hrs dipping led to improve rooting parameters particularly the rooted cuttings percent. However, the use of 1000 mg/L IBA for 20 s quick dipping also had satisfactory results, which can be introduced as a suitable treatment due to the reduction of application time and labor costs. Adding substances similar to ascorbic acid (AsAc) and hydrogen peroxide (H2O2) also improved some traits such as the root length, root fresh weight and the number of shoots. In general, the use of high concentrations of IBA (2000 mg/L), which are used by quick dipping with the use of pre-treatment with AsAc and H2O2 can lead to improve rooting traits in bougainvillea cuttings. When the cost of the plant growth regulators is important, the use of the low-concentration of IBA (400 mg/L) by long-term dipping (24 hrs) can be effective for Bougainvillea cuttings.
گل کاغذی (Bougainvilleaspectabilis) به‌دلیل داشتند گل‌های زیبا و رنگارنگ برای کاشت در فضای سبز مورد استفاده قرار می‌گیرد. راحت‌ترین و ارزان‌ترین روش تکثیر این گیاه از طریق قلمه است. ولی قلمه‌های این گیاه به‌راحتی ریشه‌دار نشده و نیاز به تیمارهای خاصی از جمله استفاده از اکسین دارند. بدین‌منظور آزمایشی در قالب طرح کاملاً تصادفی با 15 تیمار و چهار تکرار انجام و صفاتی از قبیل طول ریشه و شاخه، تعداد ریشه و شاخه، وزن تر و خشک ریشه و درصد قلمه‌های ریشه‌دار مورد ارزیابی قرار گرفت. نتایج نشان داد که اختلاف آماری معنی‌داری بین تیمارها وجود دارد. استفاده از 400 میلی‌گرم در لیتر IBA با فروبری 24 ساعت، منجر به بهبود پارامترهای ریشه‌زایی به‌ویژه درصد قلمه‌های ریشه‌دار شد. با این حال، استفاده از 1000 میلی‌گرم در لیتر IBA با فروبری سریع 20 ثانیه نیز نتایج رضایتبخشی داشت که می‌تواند به‌عنوان یک تیمار مناسب جهت کاهش زمان کاربرد و هزینه‌های کارگری معرفی شود. افزودن موادی نظیر آسکوربیک‌اسید و پراکسید هیدروژن (آب‌اکسیژنه) نیز برخی از صفات نظیر طول ریشه، وزن تر ریشه و تعداد شاخه را افزایش داد. به‌طور کلی، استفاده از غلظت‌های زیاد IBA (2000 میلی‌گرم در لیتر) به‌صورت فروبری سریع همراه با پیش تیمار قلمه با اسکوربیک‌اسید و پراکسید هیدروژن می‌تواند منجر به بهبود صفات ریشه‌زایی در قلمه‌های گل کاغذی شود. زمانی که هزینه تنظیم‌کننده‌های رشد گیاهی اهمیت داشته باشد، استفاده از غلظت‌های کم IBA (400 میلی‌گرم در لیتر) با زمان فروبری طولانی (24 ساعت) می‌تواند برای قلمه‌های گل کاغذی موثر باشد.
Abarca-Vargas, R. and Petricevich, V.L. 2018. Bougainvillea genus: A review on phyto-chemistry, pharmacology, and toxicology. Evidence-Based Complementary and Alternative Medicine, Article ID 9070927, 17 p. doi:10.1155/2018/9070927.
Ahmad, N., Ishtiaq, M. and Nabi, G. 2002. Influence of various concentrations of IBA on different types of Bougainvillea glabera var. Variegata cuttings. Sarhad Journal of Agriculture, Pakistan, 18 (3): 263-270.
Blythe, E.K., Sibley, J.L., Ruter, J.M. and Tilt, K.M. 2004. Cutting propagation of foliage crops using a foliar application of auxin. Scientia Horticultura, 103: 31-37.
Bosila, H.A., Zewil, M.A., Hamza, M.A. and Amin, M.M. 2010. Effect of auxins and ascorbic acid on propgation of Bougainvillea glabra through stem cuttings. Journal of Plant Production, Mansoura University, 1 (7): 893-901.
Danehloueipour, N., Yan, G., Clarke, H.J. and Siddique, K.H.M. 2006. Successful stem cutting propagation of chickpea, its wild relatives and their interspecific hybrids. Australian Journal of Experimental Agriculture, 46: 1349–1354.
Eed, A.M., Albana, B. and Almaqtari, S. 2015. The effect of growing media and stem cutting type on rooting and growth of Bougainvillea spectabilis plants. University of Aden Journal of Natural and Applied Sciences, 19 (1): 141-147.
Fathi, Gh. and Esmaeilpour, B. 2000. Plant growth regulators and materials. Mashhad Jihad-Daneshgahi Press, 228 p. (In Persian)
Fathi, M., Zarei, H. and Varsteh, F. 2017. The effect of natural and chemical compounds on rooting traits of bougainvillea (Bougainvillea spectabilis L.). Journal of Chemical Health Risks, 7 (3): 181-192.
Hartmann, H.T., Kester, D.E., Davies, F. and Geneve, R. 2002. Plant propagation: Principles and practices (7th edition). New York, Prentice-Hall.
Ibironke, O.A. 2019. Root initiation of Bougainvillea from cuttings using different rooting hormones. Advances in Plants and Agriculture Research, 9 (1): 121-125.
Jalili-Marandi, R. 2003. Plant propagation. The 2nd Edition. Orumieh Jihad-Daneshgahi Press. 469 p. (In Persian)
Jalilvand, H. 2003. Effect of cutting location, medium and hormone concentration on the rooting of one Juniperus species cuttings. Journal of Agricultural and Natural Resources Science of Khazar, 1: 23-33. (In Persian)
Khaled Mousa, A.L. 2003. Rooting response of "Nabali" olive cuttings to IBA concentration and collection season. Pakistan Journal of Biological Sciences, 6 (24): 2040-2043.
Khan, M.S., Khan, R.U. and Waseem, K. 2006. Effect of some auxins on growth of damask rose cuttings in different growing media. Journal of Agriculture and Social Sciences, 2(1): 13-16.
Khoshkhoui, M. 1997. Tissue culture technique for horticultural crops. (pp. 73-79). Shiraz University Press. (In Persian).
Khoshkhui, M. 2003. Plant propagation, principals and methods. Vol. II. Shiraz University Press. pp. 522-526. (In Persian)
Mehraj, H., Shiam, I.H., Taufique, T., Shahrin, S. and Jamal Uddin, A.F.M. 2013. Influence of indole-3-butyric acid (IBA) on sprouting and rooting potential of Bougainvillea spectabilis cuttings. Bangladesh Research Publications Journal, 7 (1): 44-49.
Moalemi, N. and Chehrazi, M. 2003. Effect of auxin on the rooting of the leafy and non-leafy cuttings of Bougainvillea spectabilis. In Proceeding of the 3rd Iranian Horticultural Science Congress, p. 110. (In Persian)
Norcini, J.G., Aldrich, J.H. and Mc Dowell, J.M. 1994. Flowering response of Bougainvillea cultivars to dickegulac. HortScience, 29: 282- 284.
Satisha, J., Ramteke, S.D. and Karibasappa, G.S. 2007. Physiological and biochemical characterisation of grape rootstocks. South African Journal of Enology and Viticulture, 28: 163-168.
Satisha, J., Raveendran, P. and Rokade, N.D. 2008. Changes in polyphenol oxidase activity during rooting of hardwood cuttings in three grape rootstocks under Indian conditions. South African Journal of Enology and Viticulture, 29: 98-101.
Sebastiani, L., Tognetti, R., Di Paolo, P. and Vitagliano, C. 2004. Hydrogen peroxide and indole-3- butyric acid effects on root induction and development in cuttings of Olea europaea L. cv. Frantoio and Gentilre di larino. Horticultural Science, 16 (1): 7-12.
Sgherri, C., Cosi, E. and Navari-Izzo, F. 2003. Phenols and antioxidative status of Raphanus sativus grown in copper excess. Physiologia Plantarum, 118 (1): 21-28.
Singh, K.K. 2018. A review: Multiplication of Bougainvillea species through cutting. International Journal of Chemical Studies, 6 (2): 1961-1965.
Singh, K.K., Rawat, J.M.S. and Tomar, Y.K. 2011. Influence of IBA on rooting potential of torch glory Bougainvillea glabra during winter season. Journal of Horticultural Science and Ornamental Plants, 3 (2): 162-165.
Sultana, Z. 2006. Rooting performance of stem cuttings of three ornamental plants as influenced by growth regulators. M.Sc. Thesis, Department of Horticulture and Postharvest Technology, Sher-e-Bangla Agricultural University, Dhaka-1207.
Tariq-Shah, S., Zamir, R., Muhammad, T. and Ali, H. 2006. Mass propagation of Bougainvillea spectabilis through shoot tip culture. Pakistan Journal of Botany, 38 (4): 953-959.
Tausz, M., Wonisch, A., Grill, D.M. and Jimenez, M.S. 2003. Measuring antioxidants in tree species in the natural environment: From sampling to data evaluation. Journal of Experimental Botany, 54 (387): 1505-1510.
Xiao, L. 2003. A study on cutting techniques of Bougainvillea spectabilis. Journal of Anhui Agricultural Sciences, 12: 8-15.
Yilmaz, H., Taskin, T. and Otludil, B. 2003. Polyphenol oxidase activity during rooting in cutting of grape varieties. Turkish Journal of Botany, 27: 495-498.