Evaluation of the effect of Eucalyptus and Thyme essential oils and aqueous extract of Artemisia annum and synergistic combination of pyrimethamine-sulfadiazine on acute toxoplasmosis in Balb / c mice
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)
سید رضا حسینی
1
,
آرین اسعدپور بهزادی
2
1 - گروه انگل شناسی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی ، شهرکرد، ایران؛
2 - دانش آموخته دکتری عمومی دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
Keywords: Toxoplasmosis, Thyme essential oil, Eucalyptus essential oil, Artemisia aqueous extract, Pyrimethamine sulfadiazine compound, Survival time,
Abstract :
Introduction & Objective: Toxoplasmosis is an infection caused by Toxoplasma gondii parasite and is one of the most common parasitic infections in humans and other warm-blooded animals. In this study, the effect of Eucalyptus and Thyme essential oils and aqueous extract of Artemisia as well as the synergistic combination of pyrimethamine-sulfadiazine on acute toxoplasmosis in a mouse model was investigated.Materials and Methods: In order to investigate the effect of Eucalyptus and Thyme essential oils and Artemisia aqueous extract as well as the synergistic composition of pyrimethamine-sulfadiazine on acute toxoplasmosis in a mouse model, 44 Balb / c mice with Tuxvit tux Peritoneal infection was divided into 11 groups of 4. To 9 groups of concentrations of 150 mg / kg / day, 300 mg / kg / day and 600 mg / kg / day of eucalyptus and thyme essential oils and aqueous extract of Artemisia 24 hours after infection for 7 days orally Was fed. One group was treated orally with the chemical combination of pyrimethamine (dose 10 mg / kg / day) and sulfadiazine (dose 50 mg / kg / day) for 10 hours after infection for 10 days and another group as The patient's control was considered.Results and Discussion: The results showed that there was no obvious difference in terms of survival time between the groups treated with essential oil and plant extracts and the patient control group and all died by the sixth day, while consuming mice. The chemicals all survived, and after the peritoneal fluid and brain of one of the mice in this group were inoculated separately into two groups of healthy mice intraperitoneally, it was observed that the mice given the peritoneal fluid Inoculated, all survived, while mice inoculated with brain all perished. This result indicated that the combination of pyrimethamine-sulfadiazine in this dose is quite effective on acute toxoplasmosis, but is not able to eradicate and eliminate parasitic cysts in the brain.Practical / Industrial Recommendation: It is better to do more comprehensive research on the effects of different species of these plants on Toxoplasmosis in vitro and in vivo so that an effective and safe drug against Toxoplasmosis can be presented in the world.
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