تاثیر نانو ذره سلنیوم (Nano-Se) و عصاره سبوس برنج بر جوانه زنی و برخی ویژگی-های مورفوفیزیولوژیکی Astragalus adscendens Boissier )
محورهای موضوعی : زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم
1 - گروه مرتع و آبخیزداری دانشکده منابع طبیعی و علوم زمین دانشگاه کاشان
کلید واژه: پرایمینگ, جوانه زنی, کلروفیل, محتوی آب نسبی, طول ریشه چه,
چکیده مقاله :
به منظور بررسی اثر پیش تیمار بذور گون گزی با عصاره سبوس برنج عصاره و محلول نانو ذره سلنیوم در مراحل اولیه جوانه زنی، آزمایشی به صورت فاکتوریل در قالب طرح کاملا تصادفی در 4 تکرار در سال 1396 اجرا شد. تیمار های آزمایش شامل پرایمینگ با عصاره سبوس برنج در 4 سطح (صفر به عنوان شاهد، 1/0 ، 2/0 و 5/0 درصد وزنی-حجمی ) و نانو ذره سلنیوم در 4 سطح (صفر به عنوان شاهد، 04/0، 08/0 و 1/0 درصد وزنی-حجمی ) به مدت 2 ساعت در دمای 25 درجه سانتی گراد بودند. نتایج آزمایشات نشان داد عصاره سبوس برنج، نانو ذره سلنیوم و اثر متقابل تیمارها در سطح احتمال %1 بر تمامی صفات مورد مطالعه شامل درصد جوانه‌زنی، طول ریشه‌چه، طول ساقه‌چه، ضریب جوانه‌زنی، محتوای نسبی آب، محتوای کلروفیل a،b و کلروفیل کل معنی دار بود. همچنین استفاده از عصاره سبوس برنج1/0درصد باعث افزایش 15 درصدی طول ریشه‌چه شد، اما با افزایش غلظت عصاره سبوس برنج طول ریشه‌چه و ساقه‌چه نسبت به شاهد کاهش پیدا کردند. همچنین پرایم بذور با عصاره سبوس برنج2/0درصد و نانو ذره سلنیوم08/0درصد باعث افزایش 37 درصدی طول ریشه‌چه شد. بالاترین میزان درصد جوانه‌زنی، محتوای کلروفیل a،b و طول ساقه‌چه با اعمال تیمار 1/0 درصد وزنی-حجمی عصاره سبوس برنج به همراه تیمار 1/0 درصد وزنی-حجمی نانو ذره سلنیوم به دست آمد. همچنین اعمال تیمار های ذکر شده به تنهایی نیز بر صفات مورد مطالعه اثرات مثبت و معنی دار داشتند.
In order to investigate the effect of seeds priming of Astragalus adscendens with Rice bran extract and potassium nitrate (Nano-Se) solution in the early stages of germination, a factorial experiment was conducted in a completely randomized design (CRD) with four replications conducted at the botany Lab of the Department of Biotechnology, University of Kashan, in 2017. Experimental treatments consisted of priming with Rice bran extrac solution at 4 levels (zero as control, 0. 1, 0.2 and 0.5 (percentage of weight - Volume: w/v), and Nano-Se in 4 levels (zero as control, 0.04, 0.08 and 0.1) w/v for 2 hours at 25 ° C. The results of the experiments showed that Rice bran extract, Nano-Se solution and interaction of treatments were significant at the 1 % level on all studied traits, including germination percentage, root length, stem length, germination coefficient, relative content of water, chlorophyll a, b and total chlorophyll content. Also, the use of rice bran extract 0.1% increased the 15%. of root length but with Increasing the concentrations of bran extract root and shoot length decreased compared to control. Also, seeds priming with rice bran extract 0.2% and Nano- Se 0.8% increased the root length 37%. The highest germination percentage, the content of chlorophyll a and b, and stem length were obtained by applying 0.11% w / v Rice bran extract solution with 0.1% w/v of Nano-Se . Also, applying these treatments alone had positive and significant effects on the studied traits.
[1] Aisha A H., Rizk FA., Shaheen A.M., Abdel-Mouty, M.M. 2007, Onion plant growth, bulb yield and its physical and chemical properties as affected by organic and natural fertilization. Agriculture and Biological Science, 3(5): 380-388.
[2] Arnon D.I., 1949, Copper enzymes in isolated chloroplasts, polyphenoxidase in Beta vulgaris. f Plant Physiology, 24(1):1-15.
[3] Ayub M., Ibrahim M., Noorka I.R., Tahir M., Tanveer A., Ullah, A. 2013, Effect of seed priming on seed germination and seedling growth of garden cress (Lepidium sativum L.). International Agriculture and Applied Sciences, 5(2): 1-10.
[4] Azimi M., Mesdaghi M., Farahpour M. 2005, Relationship between producing insect population and Astragalus adscendens plant criteria in Fereydounshahr area of Isfahan. Agricultural Sciences and Technology of
Isfahan University of Technology. 9(3): 243-253.
[5] Babel S., Kurniawan T.A. 2003, Low-cost adsorbents for heavy metals uptake from contaminated water: a review. Hazardous Materials. 97:219-225.
[6] Begnami CN., Cortelazzo AL. 1996, Cellular alterations during accelerated aging of French bean seeds. Seed Science and Technology 24: 295-303.
[7] Bradford K.J. 1995, Water relations in seed germination. In "Seed Development and Germination " (J. Kigel and G. Galili, Eds.). Marcel dekkerinc. New York, 351-369.
[8] Dastborhan S., Ghassemi-Golezani K. 2015, Influence of seed priming and water stress on selected physiological traits of borage. Polish Horticultural Science, 2 (27):- 159.
[9] Demir Kaya M., Games O., Atak M., Cikili Y., Kolsarici O. 2006, Seed treatment to overcome salt and drough stress during germination in sunflower (Helianthus annuus L.). European Journal Agronomy. 24: 281-295.
[10] Fateh E., Majnoun Hosseini N., Modhara Arefi H., Sharifzadeh F. 2005. Effect of some treatment of seed five rangeland spicies Genetic Research and Reproduction of Rangelands and Forests of Iran, 13(4): 38-45.
[11] Ghasemi Pirbalooti A., Golparvar M., Riaz Dehkordi B., Navid, A. 2006. The effect of different treatments on sleep defeat and germination stimulation of five species of medicinal plants in Chaharmahal and Bakhtiyari. Journal of Research. Sazandegi, 74: 176-191.[In Persian].
[12] Guan Y.J., Hu J., Wang X.J., Shao C.X. 2009, Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. Zhejiang University-Science, 10: 427-433.
[13] Hafiz Haider A., Asif Tanveer M., Nadeem A. 2012. Evaluation of some seed dormancy breaking methods on germination of Rhynchosia capitata, (Roth DC). Pakistan Weed science research, 18(4): 423-432.
[14] Haghighi M., Afifipour Z., Mozafarian M. 2012. The effect of N-Si on tomato seed germination under levels. Biology Enviromental Science, 6(16): 78-90.
[15] [15] Keeling A A., Paton I. K., Mullett JA., 1994, Germination and growth of plants in media containing unstable refuse-derived compost. f Soil Biology and Biochemistry. 26(6): 667-772.
[16] Kumar A., Singh D.P. 2005, Use of physiological indices as screening technique for drought tolerance in oil seed Brassica species. Annual Botany, 81: 413-420.
[17] Limpanavech P., Chaiyasuta S., Vongpromek R., Pichyangkura R., Khunwasi C., Chadchawan S., Lotrakul P., Bunjongrat R., Chaidee A., Bangyeekhun T. 2008, Chitosan effects on floral production, gene expression, and anatomical changes in the Dendrobium orchid. Horticuture Science, 116(1): 65-72.
[18] Liu J., Li J., Su X., Xia Z. 2014, Grafting improves drought tolerance by regulating antioxidant enzyme activities and stress-responsive gene expression in tobacco. Environmental and Experimental Botany, 107: 173-179.
[19] Obara K., Ishihara M., Ishizuka T., Fujita M., Ozeki Y., Maehara T., Saito Y., Yura H., Matsui T., Hattori, H. 2003, Photocrosslinkable chitosan hydrogel containing fibroblast growth factor-2 stimulates wound healing in healing oxidative enzymes and osmoregulation among three different genotypes of Radix Astragali at seeding stage. Colloids and Surface Science Botany, 49: 60-65.
[20] Perry DA. 1972, Seed vigour field establishment. Horticulture Abstract 42: 334-342.
[21] Qasim M., Ashraf M.M., Jamil A.M., Rehman Y.S.U., Rha E.S. 2003, Water relations and gas exchange properties in some elite canola (Brassica napus L.) lines under salt stress. Annual Application of Biology, 142(3): 307-316.
[22] Ramaza A., Hafiz I.A., Ahmad T., Abbasi N.A. 2010, Effect of priming whit potassium nitrate and Dehusking on seed germination of Gladiolus (Gladiolus alatus). Pakistan Jornale, 42(1): 248-251.
[23] Robert EH., Osei-Bonsu K. 1998, Seed and seedling vigour. In: Summerfield RJ (Ed) World Crops: Cool Season Food Legumes London, pp: 897-910.
[24] Saif Allah A. 2015, Ph.D., Dispersal and Biological of Astragalus adscendens MS.c thesis, Faculty of Agriculture. Isfahan University of Technology. 136 p.
[25] Uthairatanakij A., Teixeira da Silva J., Obsuwan K. 2007, Chitosan for Improving Orchid Production and Quality. Orchid Science and Biotechnology, 1(1): 1-9.
[26] Wang X.H., Li D.P., Wang W.J., Feng Q.L., Cui F.Z., Xu Y.X., Song X.H., vanderwerf, M. 2003, Crosslinked collagen/chitosan matrix for artificial livers. Biomaterials, 24: 3213-3220.
[27] Yassen B.T., Mamari A.L., 1995, Further evaluation of the resistance of black barley to water stress. Agronomy Journal, 174: 19-25.
Zhou Y.G., Yang Y.D., Qi Y.G., Zhang Z.M., Wang X.J.,Hu X.J. 2003, Effects of chitosan on some physiological activity in germinating seed of peanut. Plant Science, 31: 20-28.
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