Comparative Study on the Effectiveness of the Use of Two Substances in the Disinfection of the Dental Environment by Thermonebulisation

Subject Areas : Journal of Chemical Health Risks

Oscar Daniel Escobar Zabala ^{1 *} , Jorge Daniel Bautista Cerdeño ² , Jesús Alejandro Arenas Fernández Dávila ³ , Verónica Cecilia Quishpi Lucero ⁴ , Annushka M-Caviedes ⁵ , Ninoska Sanches Palomino ⁶ , Daniel Alejandro Pallo López ⁷ , Giovanna Gutiérrez-Gayoso ⁸

1 - Department of Stomatology Universidad Nacional de Chimborazo, Ecuador
2 - Department of Stomatology Universidad Nacional de Chimborazo, Ecuador
3 - Department of Stomatology Universidad Andina del Cusco, Perú
4 - Department of Stomatology Universidad Nacional de Chimborazo, Ecuador
5 - Department of Stomatology Universidad Andina del Cusco, Perú
6 - Department of Stomatology Universidad Andina del Cusco, Perú
7 - Department of Stomatology Universidad Nacional de Chimborazo, Ecuador
8 - Department of Stomatology Universidad Andina del Cusco, Perú

Received: 2024-04-11 Accepted : 2025-02-24 Published : 2025-06-15

Keywords: Quaternary ammonium, Glutaraldehyde, Microorganisms, Stomatological practice, Thermonebulisation,

Abstract :

Stomatological practice demands a high biosafety risk due to the presence of pathogenic agents due to the production of aerosols during procedures, thus urging the implementation of a disinfection means such as Thermonebulisation to significantly eliminate microorganisms, The aim of this research was to compare the effectiveness of two disinfectant substances through thermonebulisation (quaternary ammonium and glutaraldehyde), 20 sterile swab samples from surfaces in public and private dental offices were used, the procedure was performed through thermonebulisation in a period of 5 min, and the sampling was done past the hour and a half of thermonebulisation, which were taken through Stuart transport for preservation, the samples were distributed in two groups of 10 for each disinfectant, where each 90 ml of disinfectant counted with 160 ml of distilled water, 160 ml of liquid glycerin,160 mml of industrial alcohol, It was found that in a thermonebulisation process there is greater effectiveness for quaternary ammonium with a confidence level of 100% due to the total elimination of colony-forming units of microorganisms, while glutaraldehyde only reduced the microbial load, It is concluded that quaternary ammonium proved to be more effective for the disinfection of the dental practice in contrast to glutaraldehyde.

References:

1. Laheij A.M.G.A., de Soet J.J., Crielaard W., Zemouri C., Volgenant C.M.C.و 2021. Cross contamination: spatters, aerosols, and biofilm in dental practices. Ned Tijdschr Tandheelkd. 128(4), 221-227.
2. Paes G.R., Ramos J.T., Ronsani M.M., Meira T.M., 2018. Formação profissional e conhecimento sobre biossegurança de Auxiliares de Saúde Bucal dos setores público e privado. Revista da ABENO. r. Sérgio Guandalini 18(3), 43-52.
3. Thomé, Geninho; Sérgio Bernardes; Sérgio Guandalini; Maria Claudia Vieira Guimarães: "Guidelines for best practice in biosafety at the dental clinic." E book. Straumann Group (2020).
4. Santos I.G., Souza V.G.C., Silva G.T.V. da, Lourenço A.H. de T, Laxe L.A.C., Apolônio A.C.M., 2021. Biosafety in Dental Practices Versus COVID-19 Outbreak. Pesquisa Brasileira em Odontopediatria e Clínica Integrada. 21.
5. Hu Q., Ma P., Wang Y., Huang D., Hong J., Tan Y., 2022. Thermal fogging with disinfectants and antifreezes enables effective industrial disinfection in subzero cold-chain environment. Journal of Applied Microbiology. 132(4), 2673-2682.
6. Wang Y., Wu Q., Ren B., Muskhelishvili L., Davis K., Wynne R., 2022. Subacute Pulmonary Toxicity of Glutaraldehyde Aerosols in a Human In Vitro Airway Tissue Model. International Journal of Molecular Sciences. 23(20), 12118.
7. Qiu Y., Xu J., Xu Y., Shi Z., Wang Y., Zhang L., 2023. Disinfection efficacy of sodium hypochlorite and glutaraldehyde and their effects on the dimensional stability and surface properties of dental impressions: a systematic review. Peer J. 11, e14868.
8. Alkhtani F., 2023. Disinfection of polyvinyl siloxane impression material using ozone gas, 0.1% riboflavin, glutaraldehyde, and microwave irradiation and their effect on physical properties. Photodiagnosis and Photodynamic Therapy. 41, 103242.
9. Matei A., Puscas C., Patrascu I., Lehene M., Ziebro J., Scurtu F., 2020. On the Stability of Glutaraldehyde in Biocide Compositions. International Journal of Molecular Sciences. 21(9), 3372.
10. Peyneau M., de Chaisemartin L., Gigant N., Chollet-Martin S., Kerdine-Römer S., 2022. Quaternary ammonium compounds in hypersensitivity reactions. Frontiers in Toxicology. 4, 973680.
11. Boyce J.M., 2023. Quaternary ammonium disinfectants and antiseptics: tolerance, resistance and potential impact on antibiotic resistance. Antimicrobial Resistance and Infection Control. 12(1), 32.
12. Teska P., Gauthier J., Lamb J., Hug A., 2022. Powered air-purifying respirator (PAPR) disinfection and risk of surface damage from hydrogen peroxide and quaternary ammonium chloride-based disinfectants. Journal of Occupational and Environmental Hygiene. 19(8), 449-454.
13. Vereshchagin A.N., Frolov N.A., Egorova K.S., Seitkalieva M.M., Ananikov V.P., 2021. Quaternary Ammonium Compounds (QACs) and Ionic Liquids (ILs) as Biocides: From Simple Antiseptics to Tunable Antimicrobials. International Journal of Molecular Sciences. 22(13), 6793.
14. Wang G., Yang L., Jiang L., Chen J., Jing Q., Mai Y., 2022. A new class of quaternary ammonium compounds as potent and environmentally friendly disinfectants. Journal of Cleaner Production. 379, 134632.
15. Innes N., Johnson I.G., Al-Yaseen W., Harris R., Jones R., Kc S., 2021. A systematic review of droplet and aerosol generation in dentistry. Journal of Dentistry. 105, 103556.
16. Samaranayake L.P., Fakhruddin K.S., Buranawat B., Panduwawala C., 2021. The efficacy of bio-aerosol reducing procedures used in dentistry: a systematic review. Acta Odontologica Scandinavica. 79(1), 69-80.
17. Kobza J., Pastuszka J.S., Brągoszewska E., 2018. Do exposures to aerosols pose a risk to dental professionals Occupational Medicine. 68(7), 454–8.
18. Zemouri C., Volgenant C.M.C., Buijs M.J., Crielaard W., Rosema N.A.M., Brandt B.W., 2020. Dental aerosols: microbial composition and spatial distribution Journal of Oral Microbiology. 12(1),1762040.
19. Virdi M.K., Durman K., Deacon S., 2021 The Debate: What Are Aerosol-Generating Procedures in Dentistry? A Rapid Review JDR Clinical & Translational Research. 6(2), 115–27.
20. Chávez R.C., Vélez M.C., Sanmartín N.B., 2024 Biosecurity protocols in private dental practice during the COVID-19 pandemic Revista Social Fronteriza. 4(2), e42172–e42172.
21. Polednik B., 2021 Exposure of staff to aerosols and bioaerosols in a dental office Building and Environment 187,107388.
22. Geisinger M.L., Iaonnidou E., 2021. Up in the Air? Future Research Strategies to Assess Aerosols in Dentistry JDR. Clinical & Translational Research. 6(2), 128–31.
23. World Health Organization 2014 Infection prevention and control of epidemic- and pandemic-prone acute respiratory infections in health care WHO Guidelines [sin número]:[páginas no disponibles].
24. Ge Z.Y., Yang L.M., Xia J.J., Fu X.H., Zhang Y.Z., 2020. Possible aerosol transmission of COVID-19 and special precautions in dentistry. J Zhejiang Univ Sci B. 21(5), 361–8.
25. Moffett M., Young J., Stuart R., 1948 Centralized Gonococcus Culture for Dispersed Clinics: The Value Of A New Transport Medium For Gonococci And Trichomonas. Br Med J. 28(2), 421.
26. Cruz Quintana S.M., Díaz Sjostrom P., Arias Socarrás D., Mazón Baldeón G.M., 2017. Microbiota of the oral cavity ecosystems. Revista Cubana de Estomatología. 54(1), 84–99.
27. Schinköthe J., Scheinemann H.A., Diederich S., Freese H., Eschbaumer M., Teifke J.P., 2021. Airborne Disinfection by Dry Fogging Efficiently Inactivates Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), Mycobacteria, and Bacterial Spores and Shows Limitations of Commercial Spore Carriers Applied and Environmental Microbiology. 87(3), e02019–20.
28. Khan M.H., Yadav H., 2020. Sanitization During and After COVID-19 Pandemic: A Short Review Trans Indian Natl Acad Eng. 5(4), 617–27.
29. Piovesan B., Salim M., Rai N., Tagkopoulos I., 2021. Tolerance to Glutaraldehyde in Escherichia coli Mediated by Overexpression of the Aldehyde Reductase YqhD by YqhC Frontiers in Microbiology. 12(1), 680553.
30. Zambrano-Gari C.C., Luna-Fontalvo J.A., 2013. Microbial diversity present in the environment of the dental clinic of the University of Magdalena Intropica. Rev IntRopIca. 1, 61–68.