بررسي ميزان شيوع آلودگي به كريپتوسپوريديوم در دامداران وگوسفندان شهرستان بوکان، ایران
محورهای موضوعی : انگل شناسی
امیر قزلباش
1
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سهراب رسولی
2
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,
فائزه حیدربیگی
3
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ملیکا احمدی
4
1 - گروه زیست شناسی، دانشکده علوم پایه، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه ، ایران
2 - گروه پاتوبیولوژی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه ، ایران
3 - گروه پاتوبیولوژی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه ، ایران
4 - گروه پاتوبیولوژی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه ، ایران
کلید واژه: كريپتوسپوريديوم, گوسفند, دامدار, بوکان, ایران.,
چکیده مقاله :
كريپتوسپوريديوم تک ياخته اي است که موجب عفونت فرصت طلب مهمي در انسان و نشخوارکنندگان مي شود. هدف از این مطالعه بررسی ميزان شيوع عفونت كريپتوسپوريديوزيس بر اساس برخي فاكتورهاي اپيدميولوژيك در دامداران و گوسفندان شهرستان بوکان است. در این مطالعه که از تیرماه تا آذر ماه 1398 انجام شد، 218 نمونه مدفوعی از گوسفندان و 200 نمونه مدفوعی از دامداران شهرستان بوکان، ایران جمع آوری شد. پس از تهيه گسترش ، گسترش هاي حاصل با روش ذيل نلسون تغییر یافته رنگ آميزي شدند و مورد بررسی میکروسکوپی با بزرگنمایی ×100 و ×40 قرار گرفتند. در مجموع تعداد 6 نفر (3%) از دامداران و تعدا 25 مورد مثبت (46/11 درصد) از گوسفندان مورد مطالعه آلوده به كريپتوسپوريديوم تشخيص داده شدند. در مطالعه حاضر، بيشترين ميزان شيوع با تفاوت آماري معني دار در گروه سني 7-1 سال (%83/20)، افراد داراي علائم باليني (%32/26) و افرادي که مصرف کننده سبزيجات خانگي پرورش يافته با کود دامي بودند (%52/18) مشاهده شد (p<0.05) و در مورد ساير پارامترهاي مورد مطالعه، تفاوت آماري معني داري مشاهده نگرديد (p>0.05). همچنین، بین میزان آلودگی گوسفندان به کریپتوسپوریدیوم و گروه سنی رابطه آماری معنی داری وجود داشت (p<0.05). در ادامه بررسی ها مشخص شد که بین جنسیت گوسفندان و عفونت شهرستان بوکان رابطه آماری معنی داری وجود دارد (p<0.05) .
Cryptosporidium is a protozoan that causes an important opportunistic infection in humans and ruminants. The purpose of this study is to investigate the prevalence of cryptosporidiosis infection based on some epidemiological factors in stockmen and sheep in Bukan county. In this study, conducted from July to December 2018, 218 fecal samples were collected from sheep and 200 fecal samples from stockmen in Bukan county, Iran. After preparation, the samples were stained using the Modified Ziehl-Neelsen stain method and examined microscopically at 100X and 40X magnification. In total, six stockmen (3%) and 25 sheep (11.46%) were diagnosed as infected with Cryptosporidium. In the present study, the highest prevalence was observed with a significant statistical difference in the age group of 1-7 years (20.83%), among people with clinical symptoms (26.32%), and among those who consumed home-grown vegetables cultivated with animal manure (18.52%) (p<0.05). No significant statistical difference was observed in other studied parameters (p>0.05). Additionally, there is a statistically significant relationship between the level of infection in sheep with Cryptosporidium and their age group (p<0.05). Further investigations revealed a statistically significant relationship between the sex of sheep and the infection in Bukan county (p<0.05).
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