Biosynthesis of Silver Nanoparticles using Bacillus subtilis Bacterium Cultured in Corn Steep Liquor and Evaluation of its Antibacterial Activity
Subject Areas : NanoNaeimeh Faridi Aghdam 1 , Shahram Moradi Dehaghi 2 , Sirous Ebrahimi 3 , Hamed Hamshehkar 4
1 - Faculty of Chemistry, Islamic Azad University, North Tehran Branch, Tehran, Iran
2 - Department of chemistry, Tehran North Branch, Islamic Azad University, Tehran, Iran
3 - Biotechnology Research Centre, Faculty of Chemical Engineering, Sahand University of Technology, Tabriz, Iran
4 - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Keywords: Bacteria, Corn steep, Green method, Silver nanoparticles,
Abstract :
In this study, the biosynthesis of silver nanoparticles was done using Bacillus subtilis bacterium cultured in corn steep liquor (CSL) nutrient. The biosynthesized nanoparticles were characterized by several techniques including FT-IR, XRD, UV-Vis, SEM, EDX, and TEM. The absorption spectrum of the nanoparticles indicated the maximum absorption at 436 nm. The SEM image confirmed the nanoparticles had polydisperse spherical morphology (~20nm). Also, the TEM image showed the nanoparticles had spherical or elliptical shape and the approximate diameter of the particles was between 10-20 nm. Morphological studies showed that the nanoparticles were completely separated and no aggregation was observed. Moreover, XRD studies confirmed that the produced nanoparticles were crystallized in the FCC crystal lattice. The antibacterial activity results indicated that the synthesized nanoparticles had significant effect against Escherichia coli bacteria, and the inhibition zone was equal to Gentamicin. So, the production of silver nanoparticles using green method is economically very economical, and can be a method for the production of silver nanoparticles in industrial scale.
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