In vitro propagation of orchid Phalaenopsis amabilis (L.) Blume var. Jawa

Subject Areas : Biochemistry

Mozhdeh Asa ¹ , Behzad Kaviani ²

1 - Department of Horticultural Science, Islamic Azad University, Rasht Branch, Rasht, Iran
2 - Department of Horticultural Science, Islamic Azad University, Rasht Branch, Rasht, Iran

Received: 2020-04-25 Accepted : 2020-04-25 Published : 2020-04-25

Keywords: tissue culture, plant growth regulators, ornamental plants, Micropropagation, protocorm like bodies,

Abstract :

A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, Phalaenopsis amabilis (L.) Blume var. Jawa, using plant growth regulators (PGRs). Protocorm-like bodies (PLBs), as explants were cultured on Murashige and Skoog (MS) medium fortified with various concentrations of kinetin (KIN) and indole-3-butyric acid (IBA), either individually or in combination. A combination of 1.00 mg l-1 KIN + 1.00 mg l-1 IBA was found to be suitable for regeneration of most measured characteristics especially maximum PLBs regeneration (30.40/plantlet), leaf number (5.93/plantlet), and root number (8.36/plantlet) from protocorm explants. The maximum number of plantlets (11.66) was calculated on MS medium supplemented with 1.00 mg l-1 KIN + 0.50 mg l-1 IBA, followed by 1.00 mg l-1 KIN + 1.00 mg l-1 IBA (10.33). Plantlets were transplanted to pots filled with cocochips and sphagnum moss (70:30) for acclimatization and transferred to the greenhouse. Upon ex vitro transfer, 90% of plants survived.

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