Influence of Different Amounts of Molasses and Microbiologically Treated Potato Aerial Part on in vitro Gas Production

Subject Areas :

S. Najafyar ¹ , H. Paya ^{2 *} , A. Taghizadeh ³ , A. Hosseinkhani ⁴ , H. Mohammadzadeh ⁵

1 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
4 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
5 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

Received: 2024-03-14 Accepted : 2024-11-15 Published : 2024-12-01

Keywords: alfalfa hay, in vitro gas production, microbial additive, molasses, processed po-tato aerial part,

Abstract :

This project aimed to study the effects of in vitro gas production when varying amounts of molasses- and microbial additive-treated aerial part of the potato are added in place of alfalfa hay in a total mixed ration (TMR). The experiment used three treatments and three replicates in a completely randomized design. 1) control (100% alfalfa hay in the fodder part of the diet), 2) T50 (50% alfalfa hay, 50% processed aerial part of potato in the fodder part of the diet) and 3) T100 (100% processed aerial part of potato in the fodder part of the diet) were the treatments. To collect rumen fluid, two fistulated male sheep were used. After incuba-tion, the in vitro gas production amount was measured at 2, 4, 6, 8, 12, 16, 24, 48, 72 and 96 hours. After two hours of incubation, no difference was apparent between the experimental treatments. Treatments T50 and T100 showed no significant difference in the 4th to 12th hour of incubation, but both showed a signifi-cant difference from treatment control (P≤0.05). Furthermore, all three treatments (control, T50 and T100) showed a highly significant difference (P≤0.001) in in vitro gas production from the 16th to the 96th hour of incubation. Treatments control, T50, and T100 produced 52.67, 61.6, and 68.53 ml of in vitro gas per 300 mg dry matter, respectively, based on the total volume of in vitro gas production. On this basis, lambs fed TMR instead of alfalfa hay can to utilize all of the processed aerial part of potato.

References:

Alibes X., Munoz F. and Rodrigez J. (1984). Feeding value of apple pomace silage for sheep. Anim. Feed Sci. Technol. 11, 189-197.
AOAC. (2005). Official Methods of Analysis. 18th Ed. Association of Official Analytical Chemists, Gaithersburg, MD, USA.
Behzadifard H. (2011). The effect of different processing methods of potato vine on quantity changes of solanine and nutritional value (in vitro). MS Thesis. Bou-Ali Sina Univ., Hamedan, Iran.
El Khidir O.A. and Vestergaard Thomsen K. (1982). The effect of high levels of molasses in combinations with hay on digestibility of organic matter, microbial protein synthesis and volatile fatty acid production in vitro. Anim. Feed Sci. Technol. 7, 277-286.
FAOSTAT. (2020). Database of the Food and Agricultural Organization (FAO) of the United Nations. Food and Agriculture Organization of the United Nations (FAO), Rome, Italy.
Fedorak P.M. and Hrudey S.E. (1983). A Simple apparatus for measuring gas production by methanogenic cultuvesin serum bottles. Environ. Technol. Lett. 4, 425-435.
Getachew G., Makkar H.P.S. and Becker B. (2002). Tropical browses: Contents of phenolic compounds, in vitro gas production and stoichiometric relationship between short chain fatty acid and in vitro gas production. J. Agric. Sci. 139, 341-352.
Getachew G., Robinson P.H., De-Preters E.J. and Taylor S.J. (2004). Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds. Anim. Feed Sci. Technol. 111, 57-71.
Havekes C.D., Duffield T.F., Carpenter A.J. and DeVries T.J. (2019). Effects of wheat straw chop length in high-straw dry cow diets on intake, health, and performance of dairy cows across the transition period. J. Dairy Sci. 103, 254-271.
Jadhav S.J., Shama R.D. and Salunkhe D.K. (1981). Naturally occurring toxic alkaloids in food. Science. 38, 1099-1100.
Karalazos A. and Swan H. (1977). The nutritional value for sheep of molasses and condensed molasses solubles. Anim. Feed Sci. Technol. 2, 143-152.
Khazaal K., Dentinho M.T., Ribeiro R. and Orskov E.R. (1995). Prediction of apparent digestibility and voluntary intake of hays fed to sheep: comparison between using fiber components, in vitro digestibility or characteristics of gas production or nylon bag degradation. J. Anim. Sci. 61, 527-538.
MacDonald D.A. (1979). On study flow through modelled vascular stenoses. J. Biomech. 12, 13-20.
McDonald P., Edwards R.A., Greenhalgh J.F.D., Morgan C.A., Sinclair L.A. and Wilkinson R.G. (2011). Animal Nutrition. Harlow, United Kingdom.
Menk K.H. and Steingass H. (1988). Estimation of energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Rec. Dev. 28, 7-55.
Najafyar S., Salamat Doust-Nobar R., Maheri Sis N., Fartash A., Salamatazar M. and Aminipour H. (2011). Estimation of net energy and degradability kinetics of treated whole safflower seed by in vitro gas production and nylon bag methods. Ann. Biol. Res. 2, 295-300.
Noordar H. (2012). Laboratory investigation of the effects of different processing methods on the nutritional value of potato aerial parts. MS Thesis. Bou-Ali Sina Univ., Hamedan, Iran.
NRC. (2007). Nutrient Requirements of Small Ruminants, Sheep, Goats, Cervids, and New World Camelids. National Academy Press, Washington, D.C., USA.
Parfitt S.J., Dan E.P. and Jorgensen N.A. (1982). The nutritional value of pressed potato vine silage. American J. Potato. Res. 59, 415-423.
Salehi P., Mansouri H., Ibn Abbasi R., Mahakhari S., Zamani A., Kamangar H. and Kamgar K. (2008). The effect of different methods of enrichment and processing of potato aerial parts on fattening performance of male Kurdish lambs. Research Center and Natural Resources of Kurdistan Province, Iran.
SAS Institute. (2009). SAS®/STAT Software, Release 9.2. SAS Institute, Inc., Cary, NC. USA.
Van Soest P.J., Robertson J.B. and Lewis B.A. (1991). Method for dietary neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74, 3583-3597.