Sequencing and Molecular Analysis of ATP 6 and ATP 8 of Mitochondrial Genome in Khorasanian Native Chickens
Subject Areas : Camel
1 - Department of Animal Science, Ferdowsi University of Mashad, Mashad, Iran
2 - Department of Animal Science, Ferdowsi University of Mashad, Mashad, Iran
Keywords: DNA mitochondrial, Khorasanian native chicken, phylogenic analysis,
Abstract :
In order to perform breeding programs and improve production of native chickens, preserving genetic diversity in different areas of Iran is important due to the reduced available population. Genome sequencing is considered the most functional approach to determine the phylogeny relation between close populations. The aim of the present study was the evaluation of the phylogeny and genetic nucleotide sequences of ATP 6 and a partial region of ATP 8 fragments in mitochondrial genome of khorasanian native chickens. For this purpose, blood samples were randomly collected from6 khorasanian native chickens and after DNA extraction the ATP 6 and partial region of ATP 8 fragments with the length of 867 bp were amplified using specific primers. Sequencing was don according to Sanger method and based on automate system. The phylogeny tree and matrices of the genetic distances between khorasanian native chickens and other breeds for ATP 6 and ATP 8 of mitochondrial genome were drawn using the same sequences of mitochondrial genome in other available breeds in NCBI database. Results showed no haplotype difference between the studied samples sequences. Phylogeny test results revealed hat khorasanian native chicken were similar to native chicken in Laos, White Leghorn and White Plymouth Rock. Further more, the lowest genetic distance was observed between khorasanian native chicken with Phasianus colchicus and Tragopan caboti for the ATP 6and Columba livia and Francolinus pintadeanus for the ATP 8 genes.
Anderson S., Bankier A.T., Barrell B.G., de Bruijn M.H., Coulson A.R., Drouin J., Eperon I.C., Nierlich D.P., Roe B.A., Sanger F., Schreier P.H., Smith A.J., Staden R. and Young I.G. (1981). Sequence and organization of the human mitochondrial genome. Nature. 290, 457-465.
Bellagamba F., Moretti V.M., Comincini S. and Valfare F. (2001). Identification of species in animal feedstuffs by polymerase chain reaction restriction fragment length polymorphism analysis of mitochondrial DNA. J. Agric. Food Chem. 49, 3775-3781.
Bruford M.W., Bradley D.G. and Luikart G. (2003). DNA markers re-veal the complexity of livestock domestication. Nat. Rev. Genet. 4, 900-910.
Crawford R.D. (1990). Origin and history of poultry species. Pp. 1-42 in Poultry Breeding and Genetics. R.D. Crawford, Ed. Elsevier, Amsterdam.
DiMauro S. (2004). Mitochondrial diseases. Biochim. Biophys. Acta. 1658, 80-88.
Do Amaral F.S.R., Miller M.J., Silveira L.F., Bermingham E. and Wajntal A. (2006). Polyphyly of the hawk genera Leucopternis and Buteogallus (Aves, Accipitridae): multiple habitat shifts during the Neotropical buteonine diversification. BMC Evol. Biol. 6, 10-18.
Liu G.H., Shao R., Li J.Y., Zhou D.H., Li H. and Zhu X.Q. (2013). The complete mitochondrial genomes of three parasitic nematodes of birds: a unique gene order and insights into nematode phylogeny. BMC Genomics. 14, 414.
Liu G., Liu Y., Zhang H., Huang J. and Fang M. (2010). Genetic variations and sequences analysis of MTATP6 and MTATP8 genes among different Chinese pig breeds. J. Anim. Breed. Genet. 127, 474-480.
Stephens A.N., Khan M.A., Roucou X., Nagley P. and Devenish R.J. (2003). The molecular neighborhood of subunit 8 of yeast mitochondrial F1F0-ATP synthase probed by cysteine scanning mutagenesis and chemical modification. J. Biol. Chem. 278, 17867-17875.
Walker J.E., Runswick M.J., Neuhaus D., Montgomery M.G., Carbajo R.J. and Kellas F.A. (2005). Structure of the F1-binding domain of the stator of bovine F1Fo-ATPase and how it binds an alpha-subunit. J. Mol. Biol. 351, 824-838.