Insulin Like Growth Factor-1 Fortification Improves Post Thaw Sperm Quality and Fertility of Goat

Subject Areas : Camel

K. Sharma ¹ , R. Ranjan ² , S. Gupta ³

1 - Department of Animal Physiology and Reproduction, Institute of Indian Council of Agricultural Research (ICAR)-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh, India|GLA University, Mathura, UP, India
2 - Department of Animal Physiology and Reproduction, Institute of Indian Council of Agricultural Research (ICAR)-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh, India
3 - GLA University, Mathura, UP, India

Received: 2023-01-23 Accepted : 2023-05-16 Published : 2023-06-01

Keywords: Antioxidant, Malondialdehyde, IGF-1, Goat, acrosome,

Abstract :

Insulin like growth factor-1 (IGF-I) has an antioxidant effect to maintain sperm motility. The present study designed to evaluate the effect of different levels IGF-1 on semen characteristic after cryopreservation of buck semen. Fifty ejaculates were collected and were extended with tris- citric acid- fructose diluent with 10% egg yolk and 6% glycerol with sperm concentrations of 4 × 108 mL−1. The effect of IGF-1 concentration showed significant differences (P<0.05) on most of studied traits (sperm motility, live sperm count, acrosomes integrity, hypo osmotic swelling positive spermatozoa, malondialdehyde (MDA)) and were significantly (P<0.05) highest in 250 ng/mL of IGF-1in post thaw semen. The post thaw progressive motility, live, hypo osmotic swelled and acrosome intact sperm increased by 25.26%, 26.77%, 30.35% and 25.52%, respectively in 250 ng/mL IGF1supplemented group in compare with control group. The 250 ng/mL IGF-1 supplemented group showed the lowest MDA content (P<0.05) in compare with other studied groups. The fortification 250 ng/mL IGF-1 reduced the MDA content by 31.16% more than the control group. The results suggested that IGF-1 protects sperm membrane lipid peroxidation by lowering MDA production by reducing the harmful effect of reactive oxygen species. Eight out of ten goats were conceived and kidded by using supplemented of 250 ng/mL IGF-1 and three goats were kidded by non-supplemented (control) frozen semen straw through artificial insemination (AI). The success of kidding was 40.00% in supplemented group (250 ng/mL IGF-1) than 30.00% in non-supplemented group (control) on actual kidding basis.

References:

Chatterjee S. and Gagnon C. (2001). Evidence for the production of oxygen free radicals during freezing/thawing of bull spermatozoa. Mol. Reprod. Dev. 59, 451-458.

Gunaseelan M., Singh B.P., Verma M.R. and Pachaiyappan K. (2018). Adoption level of improved goat farming technologies by commercial goat farmers in Tamil Nadu, India. Int. J. Curr. Microbiol. Appl. Sci. 7, 3293-3300.

Hancock J.L. (1951). A staining technique for the study of temperature-shock in semen. Nature. 167, 323-324.

Henricks D.M., Kouba A.J., Lackey B.R., Boone W.R. and Gray S.L. (1998). Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biol. Reprod. 59, 330-337.

Jones J.I. and Clemmons D.R. (1995). Insulin-like growth factors and their binding proteins: biological actions. Endocr. Rev. 16, 3-34.

Kumar P., Suman S., Pawaria S., Dalal J., Bhardwaj S., Patil S., Jerome A. and Sharma R.K. (2019). Serum and seminal plasma IGF-1 associations with semen variables and effect of IGF-1 supplementation on semen freezing capacity in buffalo bulls. Anim. Reprod. Sci. 204, 101-110.

Kumaresan A., Ansari M.R., Garg A. and Kataria M. (2006). Effect of oviductal proteins on sperm functions and lipid peroxidation levels during cryopreservation in buffaloes. Anim. Reprod. Sci. 93, 246-257.

Lone S.A., Mohanty T.K., Baithalu R.K. and Yadav H.P. (2019). Sperm protein carbonylation. Andrologia. 51(4), 13233-13241.

Miah A.G., Salma U., Takagi Y., Kohsaka T., Hamano K.I. and Tsujii H. (2008). Effects of relaxin and IGF-I on capacitation, acrosome reaction, cholesterol efflux and utilization of labeled and unlabeled glucose in porcine spermatozoa. Reprod. Med. Biol. 7, 29-36.

Naz R.K. and Padman P. (1999). Identification of insulin-like growth factor (IGF)-1 receptor in human sperm cell. Arch. Androl. 43, 153-159.

Pan Y., Cui Y., Baloch A.R., Fan J., He J., Li G., Zheng H., Zhang Y., Lv P. and Yu S. (2015). Insulin like growth factor I improve yak (Bosgrunniens) spermatozoa motility and the oocyte cleavage rate by modulating the expression of Bax and Bcl-2. Theriogenology. 84, 756-762.

Ranjan R., Ramachandran N., Jindal S.K., Sinha N.K., Goel A.K., Kharche S.D. and Sikarwar A.K.S. (2009a). Effect of egg yolk levels on keeping quality of Marwari buck semen at refrigeration temperature. Indian J. Anim. Sci. 79, 662-664.

Ranjan R., Ramachandran N., Jindal S.K. and Sinha N.K. (2009b). Hypo osmotic swelling test in frozen thawed goat spermatozoa. Indian J. Anim. Sci. 79, 1022-1023.

Ranjan R., Goel A.K., Kharche S.D., Ramachandran N., Gangwar C. and Jindal S.K. (2014). Comparison of normal and dual staining techniques for evaluating viability and acrosome status in frozen thawed buck spermatozoa. Indian J. Small Rumin. 20, 50-53.

Ranjan R., Priyadharsini R., Goel A.K., Brejendra S., Satish K., Kharche S.D. and Jindal S.K. (2017). Effect of membrane stabilizer on the freezability of buck semen. Indian J. Anim. Sci. 87, 435-436.

Ranjan R., Goel A.K., Kharche S.D., Priyadharsini R., Ramachandran N., Singh M.K., Kumar R., Dige M.S., Bhushan S., Choudhary U.B. and Kumar S. (2020). Effect of cervical insemination with frozen semen on fertility of Indian goat breed. Indian J. Anim. Sci. 90, 557-559.

Ranjan R., Kumar M., Swain D.K., Singh S.P., Kharche S.D. and Chauhan M.S. (2022). Vitamin B7 protects DNA damage and stabilizes mitochondrial transmembrane potential from cryoinjury. Small Rumin. Res. 212, 106719-106726.

Revell S.G. and Mrode R.A. (1994). An osmotic resistance test for bovine semen. Anim. Reprod. Sci. 36, 77-86.

Selvaraju S., Binsila B., Krishnan K., Archana S.S. and Ravindra J.P. (2016). IGF-1 stabilizes sperm membrane proteins to reduce cryoinjury and maintain post-thaw sperm motility in buffalo (Bubalus bubalis) spermatozoa. Cryobiology. 73, 55-62.

Singh P., Ranjan R., Kharche S.D. and Singh M.K. (2020). Exogenous catalase improves the goat seminal attributes at refrigeration temperature. Indian J. Anim. Sci. 90, 379-382.

Spiteri-Grech J. and Nieschlag E. (1993). Paracrine factors relevant to the regulation of spermatogenesis-a review. Reproduction. 98, 1-14.

SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 20. Chicago SPSS Inc., USA.

Vickers M.H., Casey P.J., Champion Z.J., Gravance C.G. and Breier B.H. (1999). IGF-I treatment increases motility and improves morphology of immature spermatozoa in the GH-deficient dwarf (dw/dw) rat. Growth Horm. IGF Res. 9, 236-240.

Watson P.F. (1975). Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Vet. Record. 97, 12-15.

White I.G. (1993). Lipids and calcium uptake of sperm in relation to cold shock and preservation: A review. Reprod. Fertil. Dev. 5, 639-658.

Zangerônimo M.G., Silva D.M., Murgas L.D.S., Sousa R.V., Rocha L.G.P., Pereira B.A., Faria B.G. and Veras G.C. (2013). Identification of insulin-like growth factor-I in boar seminal plasma and its influence on sperm quality. Arch. Zootec. 62, 411-418.