Polymorphism and Sequencing of DGAT1 Gene in Iranian Holstein Bulls

Subject Areas : Camel

M. Hosseinpour Mashhadi ¹ , M.R. Nassiri ² , M. Mahmoudi ³ , M. Rastin ⁴ , N.E.J. Kashan ⁵ , R. Vaez Torshizi ⁶ , N. Tabasi ⁷ , S.E. Nooraee ⁸

1 - Department of Animal Science,Mashhad Branch, Islamic Azad University, Mashhad, Iran
2 - Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran
3 - Immunology Research Center, Bu Ali Research Institute, Mashhad Universityof Medical Science, Mashhad, Iran
4 - Immunology Research Center, Bu Ali Research Institute, Mashhad Universityof Medical Science, Mashhad, Iran
5 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
6 - Department of Animal Science, Tarbiat Modares University, Tehran, Iran
7 - Immunology Research Center, Bu Ali Research Institute, Mashhad Universityof Medical Science, Mashhad, Iran
8 - Department of Animal Science,Mashhad Branch, Islamic Azad University, Mashhad, Iran

Received: 2011-02-18 Accepted : 2011-04-22 Published : 2012-03-01

Keywords: PCR-RFLP, Holstein, gene, DGAT1,

Abstract :

Quantitative traits locus for milk production traits has been described on centromeric end of bovine chromosome 14. Reports name the acyl coA: diacylglycerol acyltransferase (DGAT1) gene as a potential candidate gene with dinucleotide substitution (AA to GC) in exon VIII which causes the change of lysine to alanine in amino acid (K232A).The aim of the present study was to estimate the frequency of DGAT1 K232A polymorphism in Iranian Holstein bulls as a potential quantitative trait locus (QTL) for marker assisted selection. Sample of 103 Holstein bulls from the Animal Breeding Center of Iran were genotyped for DGAT1 polymorphism (A and K allele). The PCR-RFLP technique was used to study the DGAT1 gene polymorphism. Frequency of KK, KA and AA genotypes were 0.59, 0.41 and zero respectively. The allele frequencies of the DGAT1 gene were 0.7961 and 0.2039 for K and A allele, respectively. The K allele was sequenced and registered in NCBI gene bank with EU075528 accession number.

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