تمرین دوی استقامتی شدید و مکمل‌یاری با عصاره آبی بذر زنیان: تاثیر بر سطوح روی و برخی انتقال دهنده‌های روی در بافت کبد در موش‌های صحرایی نر ویستار
محورهای موضوعی : فصلنامه زیست شناسی جانوریعباس قنبری نیاکی 1 , عراز نظری 2 , خدیجه نصیری 3
1 - گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه مازندران، مازندران، بابلسر، ایران
2 - دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه مازندران، مازندران، بابلسر، ایران
3 - گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه مازندران، مازندران، بابلسر، ایران
کلید واژه: ورزش با شدت بالا, مکمل یاری, عصاره آبی دانه زنیان, Slc30a (ZnT), Slc39a (Zip),
چکیده مقاله :
روی بعنوان یک عنصر کمیاب ضروری، نقش مهمی در متابولیسم سلول‌ها دارد. بطوریکه با اتصال به بسیاری از پروتئین‌ها، بر طیف گسترده‌ای از فرایند‌های بیولوژیکی از قبیل رشد طبیعی، تولید مثل، سنتز DNA، تقسیم سلولی، بیان ژن، سیگنالینگ سلولی، بهبود زخم، استخوان‌سازی و تقویت سیستم ایمنی بدن ضروری است. کبد، نقش اساسی در متابولیسم و هموستاز روی در بدن دارد. غلظت روی در سلول‌ها توسط دو خانواده از پروتئین‌های ناقل روی Slc30a (ZnT) وSlc39a (Zip) که به ترتیب برای کاهش و افزایش غلظت روی سیتوزولی شناخته شده‌اند به شدت تنظیم می‌شود. چهل سر موش صحرایی نر نژاد ویستار (4 تا 5 هفته، 7/8 ± 189 گرم) به‌طور تصادفی در چهار گروه: سالین- کنترل (SC)، سالین- تمرین (ST)، زنیان- کنترل (AC) و زنیان- تمرین (AT) قرار گرفتند. موش‌های گروه تمرین روی تردمیل با سرعت 32 متر در دقیقه، 60 دقیقه در جلسه، 5 روز در هفته به مدت 8 هفته روی نوارگردان دویدند. موش‌ها به‌صورت خوراکی (2 گرم در 10 میلی‌لیتر آب/کیلوگرم وزن بدن) دریافت کردند و گروه‌های سالین به همان روش تیمار شدند. برای تجزیه و تحلیل داده‌ها از نرم¬افزار SPSS نسخه 27 و روش اماری آنالیز واریانس دو طرفه و آزمون تعقیبی توکی استفاده شد. مکمل‌یاری با زنیان نسبت به گروه‌های سالین، بیان ژن Znt5 و Zip8 را بطور معنی‌داری افزایش داده بود. سطوح روی و همچنین بیان ژن Znt6 و Zip7 تغییرات معنی‌داری نداشت. اجرای همزمان تمرین و مکمل‌یاری با عصاره آبی دانه زنیان ممکن است به‌طور سطوح روی و همچنین بیان ژن برخی از ناقلان روی را در کبد تعدیل کند. این یافته‌ها می‌تواند بینش جدیدی در مورد مکانیسم‌های اساسی اثرات ورزش و تغذیه بر بافت کبد ارائه دهد.
Zinc, an essential trace element, plays a crucial role in cellular metabolism. By interacting with numerous proteins, it is essential for a wide range of biological processes, including normal growth, reproduction, DNA synthesis, cell division, gene expression, cell signaling, wound healing, bone formation, and immune system enhancement. The liver plays a central role in zinc metabolism and homeostasis in the body. Zinc concentrations in cells are tightly regulated by two families of zinc transporter proteins, Slc30a (Znt) and Slc39a (Zip), which are known to decrease and increase cytosolic zinc concentration, respectively. Forty male Wistar rats (4-5 weeks old, 189 ± 7.8 g) were randomly assigned to four groups: saline-control (SC), saline-exercise (ST), Ajwain-control (AC), and Ajwain-exercise (AT). Mice in the training group ran on a treadmill at 32 m/min for 60 minutes per session, 5 days a week, for 8 weeks. Mice were received orally (2 g in 10 ml water/kg body weight) and saline groups were treated in the same way. Data analysis was performed using SPSS version 27 software with a two-way ANOVA and Tukey's post hoc test. Ajwain supplementation significantly increased Znt5 and Zip8 gene expression compared to saline groups. Zinc levels and Znt6 and Zip7 gene expression did not show significant changes. Simultaneous implementation of exercise and supplementation with aqueous extract of fennel seeds may modulate zinc levels as well as the gene expression of some zinc transporters in the liver. These findings may provide novel insights into the underlying mechanisms of exercise and nutrition effects on liver tissue.
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