تاثیر تمرین مقاومتی بر بیان مولکول‌های چسبان سلولی و RAGE در بطن چپ رت های دیابتی شده توسط رژیم غذایی پرچرب و استرپتوزوتوسین
محورهای موضوعی : فصلنامه زیست شناسی جانوری
محمدرضا باقری افسریه ای
1
(گروه فیزیولوژی ورزشی، واحد تهران جنوب، دانشگاه آزاد اسلامی، تهران، ایران)
عبدالعلی بنائی فر
2
(گروه فیزیولوژی ورزشی، واحد تهران جنوب، دانشگاه آزاد اسلامی، تهران، ایران)
سجاد ارشدی
3
(گروه فیزیولوژی ورزشی، واحد تهران جنوب، دانشگاه آزاد اسلامی، تهران، ایران)
شهرام سهیلی
4
(گروه فیزیولوژی ورزشی، واحد شهر قدس، دانشگاه آزاد اسلامی، تهران، ایران)
کلید واژه: تمرین مقاومتی, دیابت نوع 2, بیان ژن, مقاومت انسولین, مولکول چسبان سلولی,
چکیده مقاله :
کاردیومیوپاتی دیابتی (DCM) یک شرایط پاتوفیزیولوژیک است که در پاسخ به دیابت اتفاق می¬افتد و به نارسایی قلبی منجر می‌شود. هدف از پژوهش حاضر بررسی تاثیر تمرین مقاومتی بر بیان ژن¬های RAGE، VCAM-1 و ICAM-1 در بطن چپ رت‌های چاق دیابتی نوع 2 بود. در این مطالعه تجربی، 14 سر موش نر صحرایی نژاد ویستار دیابتی شده توسط رژیم غذایی پر چرب و تزریق استرپتوزوتوسین به طور تصادفی به دو گروه کنترل و مقاومتی تقسیم شدند. پروتکل تمرینی به مدت 6 هفته به تعداد 5 جلسه در هفته در قالب 3 ست با 5 تکرار در هر ست انجام گرفت. فواصل استراحت بین ست¬ها و تکرارها در هر ست به ترتیب 90 و 30 ثانیه بود. همه موش‌ها 48 ساعت پس از آخرین جلسه تمرینی تشریح شدند. از آزمون آماری تی مستقل جهت مقایسه متغیرها در سطح معنی‌داری آلفای کمتر از 5 صدم استفاده شد. تمرین مقاومتی به تغییر معنی‌داری در بیان ICAM-1 (237/0 = p)، VCAM-1 (295/0 = p) و RAGE (561/0 = p) در مقایسه با گروه کنترل منجر نشد. با این حال به کاهش معنی‌دار گلوکز و مقاومت انسولین و افزایش معنی‌دار انسولین سرم منجر شد (001/0 = p). بر پایه یافته های مطالعه حاضر، تمرینات تناوبی در غیاب تغییر بیان ICAM-1، VCAM-1 و RAGE در بطن چپ با بهبود نیمرخ گلیسیمیک و مقاومت انسولین در رت‌های دیابتی نوع 2 همراه است.
Diabetic cardiomyopathy (DCM) is a pathophysiological condition that occurs in response to diabetes and leads to heart failure. The purpose of this study was to determine the effect of resistance training on ICAM-1, VCAM-1, and RAGE gene expression in the left ventricular in obese rats with type 2 diabetes. In this experimental study, 14 male Wistar diabetic rats made diabetic by a high-fat diet and streptozotocin were randomly divided into two control and resistant groups. The training protocol was performed for 6 weeks in 5 sessions per week in the form of 3 sets with 5 repetitions in each set. Rest intervals between sets and repetitions in each set were 90 and 30 seconds, respectively. All rats were dissected 48 hours after the last training session. The independent t-test was used to compare variables at a significance level of alpha less than 5 percent. Resistance training did not lead to a significant change in the expression of ICAM-1 (p = 0.237), VCAM-1 (p = 0.295) and RAGE (p = 0.561) compared to the control group. However, it led to a significant decrease in glucose and insulin resistance and a significant increase in serum insulin (p = 0.001). Based on the findings of the present study, intermittent exercise in the absence of changes in the expression of ICAM-1, VCAM-1, and RAGE in the left ventricular was associated with the improvement of glycemic profile and insulin resistance in type 2 diabetic rats.
1. Abdul S., Shawk R., Baher H. 2014. Intercellular adhesion molecule-1, vascular adhesion molecule-1, interlukin1-β, and c - reactive protein levels in iraqi patientswith type 2 diabetes mellitus. J Dental Med Sci, 13(4):55-62.
2. Asad M., Sistani M., Barzegari A. 2019. The Effect of Eight Weeks of Continuous Endurance Training on ICAM-1 and VCAM-1 Expression in the Heart Tissue of Rats. Journal of Babol University of Medical Sciences, 21(1):230-6.
3. Banaeifar A., Ebrahimpour S., Tabatabaei H., Ebadi Ghahremani M. 2018. The Effect of Resistance Training on Muscle GLUT4Gene expression Blood glucose and Insulin Resistancein type 2Diabetic Rats. Scientific Journal of Ilam University of Medical Sciences, 17(7):46-57.
4. Basta G., Lazzerini G., Massaro M., Simoncini T., Tanganelli P., Fu C. 2016. Advanced Glycation End Products Activate Endothelium Through Signal-Transduction Receptor RAGE, AMechanism for Amplification of Inflammatory Responses. American Heart Association, Inc, 11:816- 22.
5. Bijeh N., Zabihi A., Jafari M. 2011. The acute effects of strength training on inflammatory markers predicting atherosclerosis: a study on inactive middle-aged men. Tehran Univ Med J, 69(3):204-209.
6. Bosanská L., Michalský D., Lacinová Z., Dostálová I., Bártlová M., Haluzíková D. 2010. The Influence of Obesity and Different Fat Depots on Adipose Tissue Gene Expression and Protein Levels of Cell Adhesion Molecules. Physiol Res, 59(1):79-88.
7. Burgomaster K.A., Howarth K.R., Phillips S.M., Rakobo wchuk M., Macdonald M.J., McGee S.L. 2008. Similar Metabolic Adaptations during Exercise after Low Volume Sprint Interval and Traditional Endurance Training in Humans. J Physiol, 586(1):151-160.
8. Choi K. M., Han K.A., Ahn HJ., Hwang S.Y., Hong H.C., Choi H.Y. 2012. Effects of exercise on sRAGE levels and cardiometabolic risk factors in patients with type 2 diabetes: a randomized controlled trial. J Clin Endo-crinol Metab. 97(10):3751-8.
9. Colberg S., Sigal R., Fernhall B., Regensteiner J., Blissmer B., Rubin R. 2010. Exercise and type 2 diabetes: the American College of Sports Medicine and the American Diabetes Association: joint position statement. Diabetes Care, 33:147-67.
10. Ding Y.H., Young C.N., Luan X., Li J., Rafols J.A., Clark J.C., 2005. Exercise preconditioning ameliorates inflammatory injury in ischemicf rats during reperfusion. Acta Neuropathol (Berl), 109(3):237-46.
11. Eizadi M., Mirakhori Z., Farajtabar B.S. 2019. Effect of 8-Week Interval Training on Protein Tyrosine Phosphatase 1B Expression in Gastrocnemius Muscle and Insulin Resistance in Rats with Type 2 Diabetes. Avicenna J Med Biochem, 7(2): 51-56.
12. Farsi S., Azarbayjani M.A., Hosseini S.A., Pirei P. 2008. Response of Serum Levels of ICAM-1, VCAM- 1and CRP to Highand Moderate Endurance Trainings in Sprague Dawley MaleDiabetic Rats. Armaghane-danesh, 21(8):757-71.
13. Gu Q., Wang B., Zhang X.F., Ma Y.P., Liu J.D., Wang X.Z. 2014. Contribution of receptor for advanced glycation enproducts to vasculature-protecting effects of exercise training in aged rats. Eur J Pharmacol, 741(27):186-94.
14. Hosseini M., Bagheri Afsariehee M.R. 2016. The effect of high intensity interval training and Aloe Vera consumption on resistin and insulin resistance index in diabetic rat. Feyz, 22(4):370-8.
15. Hosseni Abrishami L., Hejazi SM., Rashid Lamir A., 2019. The Effect of Eight Weeks of Continuous and Intermittent Aerobic Exercise on Serum C-Reactive Protein and Adhesion Molecules in Men With Heart Failure. Sabzevar University of Medical Sciences, 26(4). [In persian].
16. Jamshidi K.Z., Ahmadizad S., Hedayati M., Rahmani H. 2014. Responses of visfatin and insulin resistance index to different resistance exercise protocols. Iranian J Diabetes Metab, 13:297-307.
17. Jasińska-Stroschein M. 2023. The current state of preclinical modeling of human diabetic cardiomyopathy using rodents. Biomed Pharmacother, 168:115843-115855.
18. Jia G., Hill MA., Sowers JR. 2018. Diabetic Cardiomyopathy:An Update of Mechanisms Contributing to This Clinical Entity. Circ Res, 122(4):624-38.
19. Kadoglou NP., Fotiadis G., Kapelouzou A., Kostakis A., Liapis C.D. 2013. The differen-tial anti-inflammatory effects of exercise mo-dalities and their association with early carotid atherosclerosis progression in patients with type 2 diabetes. Diabet Med, 30(2):41-50.
20. Kargarfard M., Lam E.T., Shariat A., Asle Mohammadi M., Afrasiabi S., Shaw I. 2016. Effects of endurance and high intensity training on ICAM-1 and VCAM1 levels and arterial pressure in obese and normal weight adolescents. Physician Sports medicine, 44(3):208-216.
21. Koh Y., Park J . 21018. Cell adhesion molecules and exercise. J Inflamm Res, 11:297–306.
22. Lorenzo O., Picatoste B., Egido J., Tunon J. 2011. Potential Role of Nuclear Factor κB inDiabetic Cardiomyopathy. Hindawi Publishing Corporation Mediators of Inflammation, 9(3):1-9.
23. Mohammadi R., Matin Homaee H., Aazarbayjani M.A., Baesi K. 2017. The Effect of 12-Week Resistance Training on Cardiac Hypertrophy, Glucose Level, Insulin, and Insulin Resistance Index in STZ-Induced Diabetic Rats. Qom Univ Med Sci J, 11(2):38-45.
24. Mohammadi R., Matin Homaee H., Azerbaijani M.A., Baesi K. 2016. Effect of 12 week resistance training on gene expressions rage, icam, vcam in the heart of diabetic rats with stz. Iranian journal of diabetes and lipid disorders, 16(1):1-8.
25. Nowotny K., Jung T., HOhn A., Weber D., Grune T., 2016. Advanced Glycation End Products and Oxidative Stress in Type 2 Diabetes Mellitus. Biomolecules, 5(1):194-222.
26. Olson T.P., Denge l.D. 2007. Changes in inflammatory biomarkers following one-year of moderateresistance training in overweight women. International Journal of Obesity, 31:996–1003.
27. Pearson M.J., Mungovan SF., Smart N.A. 2018. Effect of Aerobic and Resistance Training on Inflammatory Markers in Heart Failure Patients: Systematic Review and Metaanalysis. Heart Failure Reviews, 23(2):209-23.
28. Ramezani N., Gaiini A., Choobineh S., Kordi M., Baesi K. 2017. The Effect of resistance training on serum levels of RBP-4 and insulin resistance index in type 2 diabetic male rats. Journal of North Khorasan University of Medical Sciences, 9(2):147-157.
29. Riebe D., Franklin B., Thompson P., Garber C., Whitfield G., Magal M. 2015. Updating ACSM's Recommendations for Exercise Preparticipation Health Screening. Med Sci Sports Exerc, 47:2473-2479.
30. Sharman M.J., Jefe V., 2004. Weight Loss Leads to Reductions in Inflammatory Biomarkers after aVery-Lowcarbohydrate Diet and A Low-Fat Diet in Overweight Men. Clin Sci (Lond), 107(4):365-369.
31. Singh B., Arora S., Goswami B., Mallika V. 2009. Metabolic syndrome: A review of emerging markers and management. Diabetes & Metabolic Syndrome. Clinical Research & Reviews, 3(4):240-254.
32. Tinken T.M., Thijssen D.H.J., Hopkins N., 2009. Impact of Shear Rate Modulation on Vascular Function in Humans. Hypertension, 54:278-285.
33. Viguier C., Bullich S., Botella M., Fasseu L., Alfonso A., Rekik K. 2023. Impact of physical activity on brain oxidative metabolism and intrinsic capacities in young swiss mice fed a high fat diet. Neuropharmacology, 241:109730.
34. Wu H., Sheng Z.Q., Xie J., Li R., Chen L., Li G.N. 2116. Reduced HMGB 1-Mediated Pathway and Oxidative Stress in Resveratrol-Treated Diabetic Mice: A Possible Mechanism of Cardioprotection of Resveratrol in DiabetesMellitus. Oxid Med Cell Longev, 983680(10).
35. Yang S.J., Kim S., Hwang S.Y., Kim T.N., Choi H.Y., Yoo H.J. 2012. Association between sRAGE, esRAGE levels and vascular inflammation: analysis with 18F-fluor-odeoxyglucose positron emission tomography. Atherosclerosis, 220(2):402-406.
36. Yazdanpazhooh S., Banaeifar A., Arshadi S., Eizadi M. 2019. The effect of resistance training on PPARy expression in subcutaneous fat tissue of diabetic rats with high fat diet and STZ. Razi J Med Sci, 26(8):68-77.
37. Zebrack J.S. 2007. Role of Inflammation in Cardiovascular Disease: How to Use C‐Reactive Protein in Clinical Practice. Prog Cardiovasc Nurs, 17(4):174-185.
38. Zhang F., Su X., Huang G., Xin X.F., Cao E.H., Shi Y. 2017. sRAGE alleviates neutrophilic asthma by blocking HMGB1/RAGE signalling in airway dendritic cells. Scientific Reports, 7(1):1-13.