اثر عصاره گل سرخ (Rosa damascena Mill.) بر آسیب کبدی القا شده ناشی از تتراکلرید کربن در موش های صحرایی نر بالغ نژاد ویستار
محورهای موضوعی : فصلنامه زیست شناسی جانوریفاطمه زهرا دیبایی 1 , اکرم عیدی 2 , پژمان مرتضوی 3
1 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
2 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
3 - گروه دامپزشكي، واحد علوم و تحقيقات، دانشگاه آزاد اسلامي، تهران، ايران
کلید واژه: گل سرخ, Rosa damascena Mill., کبد, تتراکلرید کربن, موش صحرایی,
چکیده مقاله :
گل سرخ (Rosa damascena Mill.) متعلق به خانواده Rosaceae است که در بسیاری از کشورها از جمله ایران می روید. گل های خشک گل سرخ به عنوان دارو استفاده می‌شود و اعتقاد بر این است که شرایطی مانند سرماخوردگی، برونشیت مزمن، آسم، بیماری¬های پوستی، سرطان، زخم ها، جراحت ها، چین و چروک ها، عفونت ها و همچنین یبوست را کاهش می‌دهد. این مطالعه به منظور بررسی اثر محافظتی عصاره اتانولی گل سرخ بر مسمومیت کبدی ناشی از تتراکلریدکربن (CCl4) در موش های صحرایی بالغ نژاد ویستار انجام شد. حیوانات به 8 گروه شامل کنترل سالم، کنترل آسیب کبدی (CCl4، دو بار در هفته)، تجربی سالم (عصاره گل سرخ در دوزهای 50، 100، 200 میلی‌گرم بر کیلوگرم)، تجربی آسیب کبدی (CCl4+ عصاره گل سرخ در دوزهای 50، 100، 200 میلی‌گرم بر کیلوگرم) تقسیم شدند و به مدت 28 روز تحت درمان قرار گرفتند. پس از 28 روز، حیوانات بیهوش شدند و خون از قلب جمع آوری شد. سطح آسپارتات آمینوترانسفراز (AST)، آلانین آمینوترانسفراز (ALT) و آلکالین فسفاتاز (ALP) در سرم اندازه گیری شدند. فعالیت سوپراکسید دیسموتاز (SOD) و سطح مالون¬دی¬آلدهید (MDA) از طریق هموژن بافت کبد اندازه¬گیری شد. نتایج تحقیق حاضر نشان داد که عصاره گل سرخ باعث کاهش سطح آنزیم های کبدی از جمله ALT، AST و ALP می‌شود. همچنین تجویز عصاره گل سرخ باعث افزایشSOD و کاهش سطح MDA شد. نتایج نشان می‌دهد اثر محافظتی عصاره گل سرخ در برابر آسیب کبدی و استرس اکسیداتیو ایجاد شده توسط CCl4موثر است.
Damask rose (Rosa damascena Mill.) belongs to the Rosaceae family which grows in many countries including Iran. The dried flowers of rose are used medicinally and are believed to alleviate conditions such as colds, chronic bronchitis, asthma, skin diseases, cancer, wounds, injuries, wrinkles, infections, as well as constipation. This study was carried out to investigate the protective effect of ethanolic extract of rose on carbon tetrachloride (CCl4)-induced liver toxicity in adult Wistar rats. Animals have divided into 8 groups including normal control, liver damage control (CCl4, twice a week), normal experimental (rose flower extract at doses 50, 100, 200 mg/kg), liver damage experimental (CCl4 + rose flower extract at doses 50, 100, 200 mg/kg) and treated for 28 days. After 28 days, animals were anesthetized and blood was collected from the heart. Levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were measured in the serum. The activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) were measured in the liver homogenate.The result of the present study showed that rose extract reduced the levels of liver enzymes, including ALT, AST, and ALP. Also, administration of rose extract increased SOD and decreased MDA levels. These results indicate that rose extract is effective in protection against liver injury and oxidative stress caused by CCl4.
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