بررسی میزان باروری و سطح پلاسمایی هورمون‌های تخمدانی در میش‌های نژاد ماکویی درمان شده با سیدر و PMSG

محورهای موضوعی : فصلنامه زیست شناسی جانوری

قادر نجفی ¹ (گروه پاتوبیولوژی، دانشکده‌ی دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران)
جعفر پیش جنگ آقاجری ² (گروه علوم دامی، واحد مراغه، دانشگاه آزاد اسلامی، مراغه، ایران)

تاریخ دریافت : 1400/08/02 تاریخ پذیرش : 1400/09/03 تاریخ انتشار : 1401/09/01

کلید واژه: PMSG, میزان باروری, استرادیول (E2), پروژسترون (P4), میش نژاد ماکویی,

چکیده مقاله :

هدف این مطالعه بررسی میزان باروری و تعیین سطح پلاسمایی هورمون های تخمدانی در میش های نژاد ماکویی تحت تاثیر قرار گرفته با سیدر و گنادوتروپین سرم مادیان آبستن بود. برای مطالعه ی میزان باروری، تعداد 80 رأس میش سالم به طور تصادفی به چهار گروه تقسیم شدند. میش های گروه اول، دوم و سوم با تعداد مساوی 20 راس، در زمان سیدربرداری تک دوز هورمون PMSG (به ترتیب 300، 400 و500 واحد بین المللی) و میش های گروه چهارم با عنوان شاهد (20 راس) یک میلی لیتر نرمال سالین را به صورت عضلانی دریافت کردند. برای مطالعه ی هورمون های تخمدانی به طور تصادفی از نمونه های خونی ده میش در هر گروه استفاده شد. جهت تعیین سطح استرادیول و پروژسترون در گروه های آزمایشی، نمونه های خونی در روزهای قرارگیری و برداشت سیدر، روزفحلی پس از تزریق PMSG و سی امین روز آبستنی، از ورید گردنی تهیه گردید. برای پاسخ های فحلی تفاوت معنی داری مشاهده نگردید (05/0 p ). نرخ آبستنی مابین گروه های آزمایشی و شاهد معنی دار بود (05/0 > p). سطح پلاسمایی استرادیول در روز سیدرگذاری و روز فحلی مابین گروه های آزمایشی و شاهد معنی دار بود (05/0 > p). سطح پلاسمایی پروژسترون در روز فحلی و روز 30 آبستنی مابین گروه های آزمایشی و شاهد معنی دار بود (05/0 > p). با توجه به میزان باروری و سطح پلاسمایی هورمون های تخمدانی در گروه های آزمایشی، پروتکل سیدرگذاری و تزریق PMSG در میش نژاد ماکویی موثر بوده است.

چکیده انگلیسی:

The objective of this study is to investigate fertility rate and determine plasma levels of ovarian hormones in the Makuii breed ewe treated by CIDR and PMSG. This investigation conducted in 80 fat-tailed Makuii breed ewes during the breeding season. All animals were divided randomly into four groups then a single intramuscular (IM) injection of PMSG (group 1, 300 IU; group 2, 400 IU; group 3, 500 IU, group 4) was made apart from 1 ml normal saline solution which was used as control group at the time of CIDR removal. Blood samples collected from the jugular vein at the days of CIDR insert removal, Estrus after PMSG treatment and the day 30 of pregnancy for the determination of plasma E2 and P4 concentrations. Estrus responses were similar in all groups. Pregnancy rates were 85%, 90%, 95% and 64.7% in groups 1, 2, 3 and the control group, respectively. Pregnancy rates were higher in groups 1, 2 and 3 than in control group (P<0.05). There were significant differences (P<0.05) between the treatment groups and the control group regarding the plasma E2 levels at days of CIDR insert and estrus. Differences between the treated and the control animals in the Plasma P4 levels at day estrus and the day 30 of pregnancy were significant (P<0.05). The result of current research showed that an injection of PMSG after CIDR removal can be more effective on the fertility response of Makuii breed ewes during breeding season.

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