مقایسه کارآیی جمعیت میکروبی شکمبه و مدفوع گوسفند در برآورد ارزش غذایی دانه‌های ذرت و سورگوم با استفاده از روش تولید گاز آزمایشگاهی

محورهای موضوعی : فصلنامه زیست شناسی جانوری

ابوالفضل آقاجانزاده گلشنی ¹ (گروه علوم دامی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران)
ناصر ماهری سیس ² (گروه علوم دامی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران)
رامین سلامت دوست نوبر ³ (گروه علوم دامی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران)
یحیی ابراهیم نژاد ⁴ (گروه علوم دامی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران)
ابوالفضل قربانی ⁵ (گروه علوم دامی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران)

تاریخ دریافت : 1400/01/25 تاریخ پذیرش : 1400/05/20 تاریخ انتشار : 1400/12/01

کلید واژه: تخمیر, انرژی قابل متابولیسم, تولید گاز آزمایشگاهی, شیرابه شکمبه, سوسپانسیون مدفوع,

چکیده مقاله :

هدف از این پژوهش بررسی کارآیی تخمیر میکروارگانیسم‌های مدفوع در مقایسه با میکروارگانیسم‌های شیرابه شکمبه برای برآورد ارزش غذایی دانه های ذرت و سورگوم در نشخوارکنندگان با روش تولید گاز آزمایشگاهی است. برای انجام آزمایش تولید گاز با استفاده از دو روش شیرابه شکمبه و سوسپانسیون مدفوع؛ لیکور شکمبه و سوسپانسیون مدفوع از سه رأس گوسفند فیستوله‌‌شده توده قزل اخذ شد. نتایج این تحقیق نشان داد که در مواد خوراکی مورد آزمایش، تفاوت معنی‌داری از نظر تولید گاز در زمان‌های مختلف انکوباسیون بین دو روش یاد شده وجود نداشت. از نظر حجم گاز حاصل از بخش قابل تخمیر (A) برای دانه ذرت بین روش‌های مورد آزمایش اختلاف معنی‌داری مشاهده نشد، اما مقدار این فراسنجه در دانه سورگوم با روش سوسپانسیون مدفوع به طور معنی‌داری بیشتر از روش شیرابه شکمبه بود (05/0p <). مقادیر انرژی قابل متابولیسم، انرژی خالص شیردهی، قابلیت هضم ماده آلی و اسیدهای چرب زنجیر کوتاه برآورد شده مواد خوراکی مورد آزمایش از روی مقدار تولید گاز با استفاده از شیرابه شکمبه و سوسپانسیون مدفوع تفاوت معنی‌داری با هم نداشتند. مقدار گاز تولیدی در روش شیرابه شکمبه از روی مقدار گاز تولیدی با روش سوسپانسیون مدفوع برای دانه سورگوم و دانه ذرت با استفاده از معادلات رگرسیونی حاصل از نتایج این تحقیق به ترتیب (6353/1-X 8929/0=Ysorghum و 4097/3- X 9657/0=Ycorn) قابل برآورد است. با توجه به نتایج پژوهش به نظر می‌رسد سوسپانسیون مدفوع، جایگزین مناسبی برای شیرابه شکمبه در روش تولید گاز آزمایشگاهی جهت ارزشیابی مواد خوراکی نشخوارکنندگان می‌باشد.

چکیده انگلیسی:

This study aimed to investigate the fermentation efficiency of faecal microorganisms in comparison with rumen microorganisms to estimate the nutritional value of corn and sorghum grains in ruminants using in vitro gas production technique. To perform in vitro gas production technique with rumen liquor and faecal suspension, rumen fluid and fresh faeces were collected from three fistulated Gezel rams. The results of this study showed that there are no significant differences due to in vitro gas production between the two methods at different incubation times in experimental feedstuffs. There was no significant difference between two methods in terms of the amount of gas production from fermentable fraction (A) for corn grain, while the amount of this parameter by fecal suspension was significantly higher than that of rumen liquor in sorghum grain (P<0.05). Estimated metabolizable energy (ME), net energy for lactation (NEL), organic matter digestibility (OMD), and short chain fatty acids (SCFA) contents of experimental grains with rumen fluid and faecal suspension showed no significant differences.The amount of gas produced with rumen liquor can be successfully estimated from faeces suspension using the obtained equations Ysorghum=0.8929 X -1.6353 for the sorghum grain and Ycorn=0.9657X-3.4097 for corn grain. According to the results of the study, it seems that the animal faeces suspension has the potential to replace rumen liquor in the in vitro gas production technique for ruminants feed evaluation.

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