بررسی اثر اسید آسکوربیک و هیدرو کسی آنالوگ متیونین بر عملکرد و بافت‌شناسی کبد جوجه‌های گوشتی تحت شرایط تنش گرمایی

محورهای موضوعی : فصلنامه زیست شناسی جانوری

مهسا عرفانی ¹ (گروه علوم دامی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران)
نیما ایلا ² (گروه علوم دامی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران)
ابولفضل زارعی ³ (گروه علوم دامی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران)
علیرضا نوشری ⁴ (گروه علوم دامی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران)

تاریخ دریافت : 1400/07/29 تاریخ پذیرش : 1400/10/22 تاریخ انتشار : 1401/06/01

کلید واژه: عملکرد, جوجه گوشتی, تنش گرمایی, ویتامین C, متیونین هیدروکسی آنالوگ,

چکیده مقاله :

تحقیق حاضر با هدف بررسی اثر اسید آسکوربیک و هیدرو کسی آنالوگ متیونین بر عملکرد و بافت‌شناسی کبد جوجه‌های گوشتی تحت شرایط تنش گرمایی انجام شد. جهت انجام ازمایش از 400 قطعه جوجه نر یکروزه سویه تجاری راس 308 در قالب یک طرح کاملا تصادفی با 4 تیمار و 5 تکرار (هر تکرار حاوی 20 قطعه جوجه) استفاده شد. تیمار های آزمایشی عبارت بودند از: 1) جیره پایه بر اساس ذرت-سویا (شاهد)، 2) جیره پایه به همراه 2/0 درصد ویتامین C، 3) جیره پایه به همراه 46/0، 36/0 و 32/0 درصد متیونین هیدروکسی آنالوگ به ترتیب در دوره اغازین، رشد و پایانی و 4) جیره پایه به همراه مخلوط 2/0 درصد ویتامین C و 46/0، 36/0 و 32/0 درصد متیونین هیدروکسی آنالوگ به ترتیب در دوره اغازین، رشد و پایانی. در دوره رشد خوراک مصرفی در تیمار حاوی متیونین هیدروکسی آنالوگ افزایش معنی داری با شاهد داشت (05/0 > p). در دوره پایانی ضریب تبدیل خوراک در تیمارهایی که از مخلوط ویتامین C و متیونین هیدروکسی آنالوگ استفاده کرده بودند بهبود معنی داری با شاهد داشتند (05/0 >p ). قطر هپاتوسیت و قطر هسته هپاتوسیت در جوجه هایی که از ویتامین C و مخلوط ویتامین C و متیونین هیدروکسی آنالوگ استفاده کرده بودند کاهش معنی داری با شاهد داشتند (05/0>P). نتایج این آزمایش نشان داد که بکار بردن مخلوط ویتامین C و متیونین هیدروکسی آنالوگ نسبت به استفاده جداگانه از آن ها در جیره غذایی می تواند بر کاهش آثار منفی تنش حرارتی موثر باشد.

چکیده انگلیسی:

The present study was aimed at investigating the effect of ascorbic acid and methionine hydroxy analogue (MHA) on performance and liver histology of broilers under heat stress conditions. To perform the experiment, 400 one-day-old male chickens of commercial Ross 308 strain were used in a completely randomized design with four treatments and five iterations (each iteration contained 20 chickens). The experimental treatments included: 1) base diet based on maize-soybean (the control), 2) base diet with 0.2% vitamin C, 3) base diet with 0.46, 0.36, and 0.32% MHA, in the starter, grower, and finisher periods, respectively, and 4) base diet with a mixture of 0.2% vitamin C and 0.46, 0.36, and 0.32% of MHA in the starter, grower, and finisher periods, respectively. During the growth period, feed consumption in the treatment containing MHA had a significant increase compared to the control group (P <0.05). In the final period, feed conversion ratio in treatments using a mixture of vitamin C and MHA had a significant improvement compared to the control group (P <0.05). Hepatocyte diameter and hepatocyte nucleus diameter were significantly reduced in chickens receiving vitamin C and a mixture of vitamin C and MHA compared to the control group (P <0.05). The results of this experiment revealed that the use of a mixture of vitamin C and MHA compared to their separate use in the diet could be effective in reducing the negative effects of heat stress.  

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