بررسی اثر داروی سیکلوفسفامید بر بیان ژن‌های ODF1 و ZPBP در بافت بیضه موش های نر نژاد ویستار
محورهای موضوعی : فصلنامه زیست شناسی جانوریصادق بازوند 1 , طاهره ناجی 2 , عبدالرضا محمد نیا 3
1 - گروه علوم پایه، دانشکده داروسازی و علوم داروئی، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه علوم پایه، دانشکده داروسازی و علوم داروئی، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران
3 - مرکز تحقیقات بیماری های مزمن تنفسی، پژوهشکده سل و بیماری‌های ریوی، بیمارستان مسیح دانشوری، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران
کلید واژه: سیکلوفسفامید, ژن ODF1, ژنZPBP, عامل آلکیله, ریل تایم,
چکیده مقاله :
سیکلوفسفوماید یک داروی الکیله کننده DNA با خواص آنتی نئوپلاستیک است .علیرغم کاربردهای کلینیکی فراوان در درمان سرطان، این دارو دارای اثرات سمی بر بافت های بدن به ویژه ارگان های جنسی می باشد. یکی از مهمترین عوارض جانبی آن، تغییر عملکرد سیستم تناسلی در جنس نر است که ممکن است منجر به ناباروری گردد. در این تحقیق تعداد 20 سر موش نر بالغ نژاد ویستار با وزن 30 ± 220 گرم انتخاب شدند و همه حیوانات توسط رژیم پلت استاندارد تغذیه گردیدند گروه دریافت کننده سیکلوفسفامید: دریافت پلیت استاندارد + آب و سیکلوفسفامید با دوز 40 میلی گرم بر کیلوگرم بصورت داخل صفاقی به مدت 30 روز تحت تیمار قرار گرفتند. بیان ژن های ODF1 وZPBP با روش ریل تایم مورد بررسی قرار گرفت. نتایج به دست آمده با استفاده از نرم افزار SPSS وآزمون آماری واریانس یک طرفهOne way ANOVA مورد تجزیه و تحلیل قرار گرفت. نتایج حاصل نشان داد که در تیمارهای آزمایشی سطح بیان ژن های ODF1 و ZPBP میان گروه های کنترل و تیمارها دارای اختلاف معناداری بود (05/0 p ≤). نتایج نشان داد که بیان ژن ZPBP و ODF1 در گروه مواجهه شده با داروی سیکلو فسفاماید نسبت به گروه کنترل به ترتیب 26/7 و 5/7 برابر کاهش داشته است و بیان ژن های فوق دستخوش تغییرات معناداری شده بود. همچنین مقایسه تعداد اسپرم ها در دو گروه کنترل و تیمار نشان داد در گروه تیمار تعداد اسپرم ها، نسبت به گروه کنترل کاهش یافته است که احتمالاً کاهش تعداد اسپرم ها در میزان باروری رت های نر نژاد ویستار موثر می باشد.
Cyclophosphoma iodine is a DNA-inducing drug with anti-neoplastic properties. One of its most important side effects is changing the function of the reproductive system in males, which may lead to infertility. The ODF gene significantly reduced in infertile men. In spite of the effect of this drug on testicular tissue, there was no work on the expression of ODF1 and ZPBP genes in testicular tissue; this was done for the first time. In this study, 20 adult male Wistar rats weighing 220±30 g were selected and all were fed by standard pellet diet in the group receiving cyclophosphamide: receiving standard pellet with water and cyclophosphamide at a dose of 40 mg/kg. The groups were treated intraperitoneally for 30 days. The expression of ODF1 and ZPBP genes was investigated by Real Time method. The results showed that the expression level of ODF1 and ZPBP genes was significantly different between the control and treatment groups, and according to the calculations performed by the software the statistical data (P≤0.05), SPSS, and one-way ANOVA test, it was concluded that ZPBP and ODF1 gene expression decreased 7.26 and 7.5 times in the group exposed to cyclophosphamide compared to the control group, respectively. Moreover, comparing the number of sperm in the control and treatment groups showed that the number of sperms in the treatment group decreased compared to the control group. Reduced number of sperm may affect the fertility rate of male Wistar rats.
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