The use of various plant materials for the synthesis of nanoparticles is considered as green synthesis, which does not involve any harmful chemicals. In this study, silver nanoparticles were synthesized by a fast, one-step method and a green biosynthesis method by regenerating a silver nitrate solution using Oak peel extract containing biomolecule which as a reducing and stabilizing element Suitable to play a role. Effective parameters such as pH of solution, silver nitrate concentration, temperature and contact time were studied and optimized The structure and properties of nanoparticles were determined by spectroscopic absorption analyzes UV-Visible, Scanning electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. According to the Sherer-Debye equation, the size of the nanoparticles was measured 29 nm. Then the antibacterial effect of nanoparticles produced against two strains of pathogenic bacteria, gram positive Staphylococcuas aureus and gram negative Escherichia coli was investigated by disk diffusion and determination of minimum inhibitory concentration(MIC) methods. The results of the antibacterial activities test showed that the nanoparticles produced from Oak Peel had a good effect on both bacteria. The purpose of this research is to synthesis and develop a new method for the preparation of silver nanoparticles using environmental methods. تفاصيل المقالة