The nursing ability of productive ewes has been a subject of extensive research. From today's perspective, the survivability of multiple lambs of highly productive ewes in birth time depends largely on the number of functional teats. Different expression patterns in a range of candidate genes may act as a regulatory sig-nal for a particular phenotype from a molecular genetic perspective. A molecular study was developed to validate the supernumerary teats characteristics. The second research phase includes a case-control applica-tion study to analyze the gene expression patterns of fibroblast growth factor 2 (FGF2) and expression of estrogen receptor 1 (ESR1) genes. The approach utilized standard laboratory techniques for RNA extraction, cDNA synthesis and cyber green dye-based gene expression quantification. When comparing two routine teat groups with four teats observation of the gene expression profile clearly shows the FGF2 gene pattern (P<0.01), There was no obvious difference in ESR1 gene response between the two experimental groups. This means that by combining promising gene expression tools, molecular approaches and evidence to vali-date gene expression in ewe carry supernumerary teats which will ultimately improve sheep production. Future research could provide valuable insights into the literature. تفاصيل المقالة