In order to evaluate of injection of different levels of vitamin E-selenium supplementation in late pregnancy of cows and its effects on growth performance and immunity of their male calves, 28 Holstein dairy cows 28 days before calving in a complete design were randomly selected with 7 replications and 4 treatments. Experimental treatments included injection of 0, 15, 30 and 45 mL of E-selenium supplementation before calving. Injections of selenium (sodium selenite) and vitamin E (DL alpha tocopherol acetate) supplements were given at 28 and 14 days before delivery. Blood samples were taken from calves at the end of the ex-perimental period (45 days after birth) to measure blood parameters. The results showed that injection of vitamin E-selenium solution in pregnant cows did not affect birth weight, feed intake and feed conversion ratio and dry matter digestibility of calves, while the final weight of calves increased (P<0.05). Blood pa-rameters of glucose, albumin, cholesterol, globulin and urea were not significantly affected, but injection of vitamin E-selenium in pregnant cows increased blood triglyceride concentration compared to the control group (P<0.05). The results showed that the glutathione peroxidase concentration in calves was signifi-cantly increased by injecting vitamin E and selenium solution in pregnant cows (P<0.05). Also, triiodotron-ine and tetraidotronin hormones concentration was significantly increased by injecting 15 mL of vitamin and selenium solution in pregnant cows compared to the control group. However, betahydroxybutyrate, malondialdehyde and non-esterified fatty acids concentrations were not affected by solution injection. The results showed that skeletal growth of calves was not affected by injection of vitamin E-selenium solution in dry cows. In general, it is concluded that injection of vitamin E-selenium in pregnant cows could im-prove the final weight, glutathione peroxidase concentration, the hormones triiodotronine and tetraiodotron-ine in calves. تفاصيل المقالة