A total 480 female and 80 male Khaki Campbell ducks were used to investigate the effect of enzyme complex on production performance and intestinal morphology in 24 weeks trial on wheat-based layer diets. There were four dietary treatment groups with 5 replicates in each. In T1 group, ducks were fed basal diet and in T2, T3 and T4 enzyme complex were supplemented at the level of 0.04%, 0.05% and 0.06%. Results showed that there were no significant differences among experimental diets on body weight (BW) and feed intake. However, a significant difference (P≤0.05) on feed conversion ratio (FCR) were observed between T4 and T1. Hen day egg production (HDEP) and hen house egg production (HHEP) were significantly (P≤0.05) improved in T4 compared to other groups. Egg mass was significantly improved (P≤0.05) in T4 than other groups. Dead germ and dead in-shells% were not affected by adding enzyme supplementation. Whereas, significant differences (P≤0.05) in fertility rate and infertile egg % were recorded between enzyme supplemented and T1 group. Hatchability % of total eggs set was significantly different (P≤0.05) in T4 whereas hatching % of fertile egg was unaffected by dietary treatments. Egg quality traits in terms of yolk weight, albumen width, shell weight, egg width, egg length, shape index and shell thickness did not differ significantly (P>0.05) among groups. Villi height (µm) and villi area (mm2) have significantly (P≤0.05) increased in the T3 and T4. The results indicated that 0.06% enzyme complex supplementation in the diet may be beneficial for egg production, hatchability, intestinal morphometry but have no effect on egg quality traits in Khaki Campbel duck. تفاصيل المقالة