Recently, Functionally Graded Scaffolds (FGSs) have attracted a lot of attention as bone replacements due to their gradient porosities as well as a bone structure. In present study titanium functionally graded scaffolds (FGSs) were fabricated by powder metallurgy route using Mg and carbamide as space holders. The arranged layers with 20, 40 and 60 Vol.% porosities were compacted in steel die using uniaxial pressure of 500 MPa before sintering in sealed quartz tubes at 1100 °C for 3 hours. Image analyzing results and scanning electron microscope (SEM) observations showed more regular shapes and sizes of pores in FGSs using Mg as a space holder compared to carbamide. The observed compressive strength and Young’s moduli of the FGSs having Mg as a space holder were in the range of 47- 160 MPa and 25-75 GPa , respectively which can be appropriate as bone replacements. The results of MTT assay showed that the values of proliferation rate were higher in samples produced using Mg. تفاصيل المقالة

Ti–6Al–4V scaffolds were fabricated by powder metallurgical space holder technique in this research. The most added magnesium (Mg) powder was evaporated and a skeleton of Ti-6Al-4V alloy was produced. For this purpose Ti-6Al-4V and Mg powders mixture compacted in a steel die by applying uniaxial pressure of 500 MPa before sintering the green product in a sealed quartz tubes at 900 °C for 2 hours. Employing Archimedes’ principle and an Image Tool software, the total and open volume percent of porosities within the scaffolds were found to be in the range of 46-64% and 41-47%, respectively. Bioactivity properties of the scaffolds were investigated in a simulated body fluid (SBF). Scanning electron microscopy (SEM) and Energy dispersive spectroscopy (EDS) were used for studying the specimens’ surfaces after immersing them for 28 days. The results showed that the amounts of calcium (Ca) and phosphor (P) deposited into the porous areas were more than that of smooth surfaces due to the presence of Mg particles within the pores which provoked formation of apatite layers. Changing in the pH values of the SBF during 18 days of immersion revealed that gradual improvement in pH level due to releasing OHˉ .Using atomic absorption spectroscopy (AAS) indicated that by increasing Mg content of scaffolds, Ca concentration of SBF decreased which is an indication of apatite formation on the scaffold. Results of SBF bioactivity assays exhibited that the scaffold with 60 vol.% Mg has the best ability to induce apatite nucleation. تفاصيل المقالة