Synthesis and Characterization of Chitosan/ Soya Protein /clay nanocomposite bioFilm for the Release of the Drug
الموضوعات : Journal of Nanoanalysisfatemeh Mirjalili 1 , Mahboobeh Mahmoodi 2 , fatemeh Sadeghian-Nodoushan 3 , Seyyed Ahmad Mirzababaieiy 4
1 - Department of material engineering,faculty of engineering,Islamic azad university Maybod beranch,Iran
2 - Department of Biomedical Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran | Joint Reconstruction Research Center, Tehran University of Medical Sciences, Tehran, Iran
3 - Medical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Science Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4 - Department of Biomedical Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran
الکلمات المفتاحية: Chitosan , soya protein, valsartan, nano clay particle,
ملخص المقالة :
Soya protein is one of the most easily available biodegradable polymers. However, there are some drawbacks that limit its application. Recently, soya protein-based nanocomposite bio film has proven to be a promising option in improving properties. In this study, Chitosan/ Soya Protein /clay nanocomposite biofilm containing valsartan as a drug was fabricated by solvent mixing method. Then, the nano biofilm properties such as surface morphology, chemical structure, swelling ratio, degradation rate and the release rate of valsartan were measured. The results showed that, the formation of a chemical bond in the constituents along with a smooth monolithic and compact surface. In addition, the swelling and degradation tests indicated that, the addition of 0.1 g of clay nano particle to the biofilm resulted in an increasing in the swelling and biodegradation rate and with addition of 0.05 g of nano clay particle a significant decrease in the rate of degradation and swelling of the biofilm was archived. The drug release test results also showed that, no sever release by soya protein /chitosan/clay nano composite biofilm containing valsartan as well as the drug release indicated a uniform release of nano composite biofilms with 0% and 0.05% of nano clay particles. Then, MTT assay was evaluated, which showed no cytotoxicity results in any of the nano biofilms. Therefore, nano composite biofilms with 0% and 0.05% of nano clay may be a more suitable option for valsartan release through the nano composite bio film for lowering the blood pressure.
[1] Tarkhan M, Safarina M , Khosh. Effectiveness of Group Stress Inoculation Training on the Systolic and Diastolic Blood Pressure and Life Quality of Hypertension in Women. Quartely Journal of heal psychology. 2012 ; 1;46 - 58.
[2] Preetishree N, Sanjib Kumar S, Anamika B. Synthesis and Characterization of Soy Protein Isolate/MMT Nanocomposite Film for the Control Release of the Drug Ofloxacin. World Journal of Nano Science and Engineering.2011; 1:27-36
[3] Michel I.Guidelines of the French Society of Otorhinolaryngology (SFORL). Epistaxis and high blood pressure. European annals of otorhinolaryngology, Head and Neck Diseases.2017; 134.1: 33-35.
[4] Safaeian L, Zabolian H. Antihypertensive Effect of Lactoferrin on Dexamethasone-Induced Hypertension in Rat. Journal of Isfahan Medical School. 2013; 31(245):1096-1104.
[5] Zarneshan A. Synergic Effect of Aerobic Exercise Training (moderate intensity) and Soy Intake on Blood Pressure and Rest Heart Rate in Obese Postmenopausal Women. Hormozgan Medical Journal.2014; 18.1: 52-60.
[6] Khedr M, Waly A.I, Hafez A.A.H. Synthesis of Modified Chitosan-Montmorillonite Nanocomposite. Aus. J. Basic App. Sci. 2012; 6:216-226.
[7] Wen-Fu L, Yung-Chu C. Effect of Intercalated Hydrotalcite on Swelling and Mechanical Behavior for Poly(acryl acid-co-N-isopropylacrylamide)/Hydrotalcite Nanocomposite Hydrogels . Applied polymer. 2005; 98:1572-1580.
[8] Su J F , Huang Z,Yuan X Y , Wang X Y , Li M. Structure and Properties of Carboxymethyl Cellulose/Soy Protein Isolate Blend Edible Films Crosslinked by Maillard Reactions. Carbohydrate polymers. 2010 ; 79(1): 145-153.
[9] Garima T, Amrinder S, Inderbir S. Chitosan-Montmorillonite Polymer Composites: Formulation and Evaluation of Sustained Release Tablets of Aceclofenac. Sci. Pharm. 2016; 84: 603–617.
[10] Chhavi M, Deepmala KM, Singh VK, Sakshi C, Naman J. Soy Protein Based Green Composite: A Review. Research & Reviews: Journal of Material Sciences. 2017;1-13.
[11] Khedr M A, Waly A, Hafez A A H. Synthesis of modified chitosan-montmorillonite nanocomposite. Aus. J. Basic App. Sc.2012; 6: 216-226.
[12] Moon M C. Perivascular Delivery of losartan with Surgical Ffibrin Glue Prevents Neointimal Hyperplasia after Arterial Injury. Journal of vascular surgery.2004; 40.1: 130-137.
[13] Gisela T. Losartan Versus Atenolol for Prevention of Aortic Dilation in Patients with Marfan Syndrome. Journal of the American College of Cardiology.2018; 72.14: 1613-1618.
[14] Wang S F , Shen L , Tong C , Phang I Y , Lim P Q , Liu T X.Chitosan/montmorillonite nanocomposites: preparation and characterization. Polymer Degradation and Stability. Biopolymer.2005; 90(1):123-131.
[15] Casettari L, Illum L. Chitosan in nasal delivery systems for therapeutic drugs. Journal of Controlled Release. 2014;190: 189-200.
[16] Akiladvi D, Basak S.Kiladevi D. Ethosomesa Noninvasive Approach for Transdermal Drug delivery. Int J Curr Pharm Res. 2010; 2.4: 1-4.
[17] Maxwell A, Priya S .Nanosized Ethosomes–A Promising Vesicular Drug Carrier for Transdermal Drug Delivery. Research Journal of Pharmacy and Technology.2019; 12.2: 876-880.
[18] Shaikh H K, Kshirsagar R, Patil S. Mathematical models for drug release characterization: a review. WJPPS. 2015; 4, 324-338.
[19] Sun X , Sun P , Li B , Liu Y , Wang M , Suo N , Jin X. A new drug delivery system for Mitomycin C to improve intravesical instillation. Materials & Design.2016; 110: 849-857.
[20] Sun M , Su X , Ding B , He X , Liu X , Yu A , Zhai G. Advances in nanotechnology-based delivery systems for curcumin. Nanomedicine. 2012; 7(7): 1085-1100.
[21] Anitha A , Sowmya S , Kumar P S , Deepthi S , Chennazhi K , Ehrlich H , Jayakumar R. Chitin and Chitosan in Selected Biomedical Applications. Progress in Polymer Science. 2014;39(9):1644-1667.
[22] Li X , Chen S , Zhang B , Li M , Diao K , Zhang Z , Chen H. In Situ Injectable Nano-Composite Hydrogel Composed of Curcumin, N, O-carboxymethyl Chitosan and Oxidized Alginate for Wound Healing Application. International journal of pharmaceutics. 2012; 437(1):110-119.
[23] QIN Z. Transdermal Permeability of Triamcinolone Acetonide Lipid Nanoparticles. International Journal of Nanomedicine. 2019; 14: 2485-2490.
[24] Mendes I T. Development and Characterization of Nanostructured Lipid Carrier-Based Gels for the Transdermal Delivery of Donepezil. Colloids and Surfaces B: Biointerfaces. 2019; 177: 274-281.
[25] Xiaoying W , Yumin D , Jiwen L. Biopolymer/Montmorillonite Nanocomposite: Preparation, Drug-Controlled Release Property and Cytotoxicity , Nanotechnology.2008; 19 :1-7.
[26] B. Karen, J. Eun ,R. Chung, N Shah, Investigation of Soy Protein Hydrogels for Biomedical Applications: Materials Characterization, Drug Release, and Biocompatibility . Journal of Biomaterials Applications.2014; 28,1 -12.
[27] Bahri-Najafi R. Designing of the Iibuprofen Mucoadhesive Film for Relief the Oral Pain and Inflammation,. Journal of Shahrekord University of Medical Sciences. 2014; 15(6): 132-140.
[28] Silva S S , Popa E G , Gomes M E , Cerqueira M , Marques A P , Caridade S G , Reis R L . An Investigation of The Potential Application of Chitosan/Aloe-based Membranes for Regenerative Medicine. Acta Biomaterialia. 2013; 9(6), 6790-6797.
[29] Liu Y , Cai Y , Jiang X , Wu J, Le X.. Molecular Interactions, Characterization and Antimicrobial Activity of Curcumin–Chitosan Blend Films. Food Hydrocolloids. 2016; 52: 564-572.
[30] H. Hosseini, B. Shirkavand Hadavand , Synthesis and Viscoelastic Properties of Smart Hydrogel. Polymer Science, Series B. 2020 ; 62, 394 -399.
[31] YU, Meiling, et al. Intratumoral Injection of Gels Containing Iosartan Microspheres and (PLG-g-mPEG)-Cisplatin Nanoparticles Improves Drug Penetration, Retention and Anti-Tumor Activity. Cancer letters. 2019, 442: 396-408.
[32] Maxwell A ,Priya S. Nanosized Ethosomes–A Promising Vesicular Drug Carrier for Transdermal Drug Delivery. Research Journal of Pharmacy and Technology. 2019; 12.2: 876-880.
[33] Anchis R, Novel Hydrogel-Advanced Modified Clay Nanocomposites as Possible Vehicles for Drug Delivery and Controlled Release. Nanomaterials.2017; 7.12: 443-448.
[34] Wang S F, Shen L, Tong C L , Phang I Y , Lim P Q , Liu T X. Biopolymer Chitosan/Montmorillonite Nanocomposites: Preparation and Characterization. Polymer Degradation and Stability. 2005; 90(1): 123-131.
[35] Asgarzade M, Shahsavari SH . The Design of a Slow-Release Drug System, 5-Fluorouracil, Using Chitosan Magnetic Nanoparticles in the Monolute Glycerol Matrix. Iranian Journal of Pharmaceutical Research. 2018;1320-1344.
[36] Ounissa S.Optimization of Valsartan Encapsulation in Biodegradables Polyesters Using Box-Behnken Design, Materials Science and Engineering C.2018;1-10.
[37] Jha A K , Tharp K M , Ye J , Santiago-Ortiz J L , Jackson W M , Stahl A , Healy K E .Enhanced Survival and Engraftment of Transplanted Stem Cells Using Growth Factor Sequestering Hydrogels. Biomaterials. 2015; 47, 1-12.
[38] Tsai Y M , Chien C –F, Lin L C, Tsai T H. Curcumin and Its Nano-Formulation: the Kinetics of Tissue Distribution and Blood–Brain Barrier Penetration. International Journal of Pharmaceutics.2011; 416(1): 331-338.
[39] Scherf, K.Martina, B.Axel, S.André , The Contamination of Valsartan and Other Sartans, Part 2: Untargeted Screening Reveals Contamination with Amides Additionally to Known Nitrosamine Impurities. Journal of Pharmaceutical and Biomedical Analysis.2019; 172, 278 -284.
