بررسی اثر عصاره آبی کلزا بر میزان کلروفیل، فعالیت آنزیمهای نیترات ردوکتاز، کاتالاز و پراکسیداز در دانهرست سویا تحت شرایط هیدروپونیک
الموضوعات :
1 - گروه زیستشناسی دانشگاه آزاد اسلامی واحد گرگان
2 - گروه زیستشناسی دانشگاه آزاد اسلامی واحد گرگان
الکلمات المفتاحية: کاتالاز, کلزا, سویا, کلروفیل, هیدروپونیک, پراکسیداز, نیترات ردوکتاز,
ملخص المقالة :
گیاه کلزا حاوی ترکیبات آللوشیمیایی میباشد که این ترکیبات متابولیسم علفهای هرز و گیاهان زراعی را تحت تاثیر قرار میدهد. هدف از این مطالعه بررسی اثر عصاره آبی کلزا بر میزان کلروفیل و نیز فعالیت آنزیمهای نیترات ردوکتاز کاتالاز و پراکسیداز در ریشه، ساقه و لپه دانهرست سویا تحت شرایط هیدروپونیک میباشد. در این راستا بذرهای گیاه کلزا رقم هایولا 401 تحت شرایط گلدانی کشت و از کل گیاه در مرحله 5 برگی عصاره آبی تهیه شد. این عصاره به محیط کشت هوگلند افزوده و پس از 9 روز میزان کلروفیل a و b در لپه و فعالیت آنزیمهای نیترات ردوکتاز، کاتالاز و پراکسیداز در سه بخش ریشه، ساقه و لپه دانهرست سویا رقم گرگان 3 مورد ارزیابی قرار گرفت. نتایج نشان داد که مقدار کلروفیل a وb در لپهها و نیز فعالیت آنزیمهای کاتالازو پراکسیداز در ریشه، ساقه و لپهدانه رستهای سویا تحت تیمار در مقایسه با شاهد (هوگلند) کاهش یافت، در حالی که فعالیت نیترات ردوکتاز در لپه کاهش و در ریشه و ساقه این گیاهان افزایش یافت.
Appel, H. M. (1993). Phenolics in ecological interactions: The importance of oxidation. Chem. Ecol. 19: 1521-1552.
Bais, H.P., Vepechedu. R., Gilroy. S., Callaway. R.M. and vivanco. J.M. (2003) Allelopathy: From molecules and genes to species interactions. Science 301: 1377-1380.
Bruisma, j. (1963).The quantitative analysis of chlorophyll a & b in plant extract .Photochem. Photobiol, 12: 241.249.
Brunn, S.A., Muday, G.K. and Haworth, P. (1992). Auxin transport and the interaction of phytotropins. Plant Physiol. 98: 101-107.
Chance, B., and Maehley, A. (1955). Assay of catalases and peroxidase, Methods in Enzymology, 2, 764-775.
Ervin, G.N. and Wetzel, R.G. (2000). Allelochemical autotoxicity in the emergent wetland macrophyte Juncus effusus ( Juncaceae ) Am. J. Bot. 87: 853-860.
Fettell, N.A. and H. S. Gill 1995. "Long-term effects of tillage, stubble, and nitrogen management on properties of a red-brown earth." Australian Journal of Experimental Agriculture 35: 923-928.
Jimenez-orornio, J.J. and Gliessman, S.R. (1987). In allelochemicals role in agriculture and foresty. American Chemical Society, Washington, Dc. pp: 262-274.
Katalin, N. Omarov, R.T., Evdei, L. and Herman lips, S. (2000). Distribution of the Mo-enzymes. aldehyde oxidase, xanthine dehydrogenase and nitrate reductase in maize (Zea maize L.) roots as affected by nitrogen and Salinity. Plan Sci. 155: 45-58.
Khalid, Sh., Ahmad, T. and Shad, R.A. (2002). Use of allelopathy in agriculture. Asian Journal of Sciences. 3: 292-297.
Koroi, S.A.A. (1989). Gele electrophores tishe and spectrophoto metrscho unter uchungen zomeinfiuss der tem pelature auf straktur der amylase and peroxidose isoenzyme. Physiol.Veg, 20: 15-23.
Malinda, D. K. (1995). Factors in conservation farming that reduce erosion. Australian Journal of Experimental Agriculture 35: 969-978.
Matile, P. Hortensteiner, S., Thomas, H. and Krautler, B. (1996). Chlorophyllase in the chloroplast envelope. Planta. 201: 96-99.
Narwal, S.S and Tauro, P. (1996). Suggested methodology for allelopathy: field observations and Methodology. P. 255-260.
Purvis, C. E. (1990). Differential response of wheat to retained crop stubbles. I. Effect of stubble type and degree of decomposition". Australian Journal of Agricultural Research 41: 225-242.
PengS-L and Shao. H. (2001). Reaserch significance and foreground of allelopathy.Chin J Appl Ecol, 12:780-786.
Rice, E. L. (1984). Allelopathy. 2nd ed. Academic press, Orland. pp: 226-291
Rizivi, S. J. H. and Rizivi. V. (1992). Expoitation of allelochemiocals in improving crop productivity. Cand hall, London. p: 443-473
Shiming, L. (2003). Allelopathy in South china agroecosystems. Institute of Tropical and Subtropical Ecology. P: 40-54
Sym, G.L. (1984). Optimisation of the invivo assay conditions for nitrate reductase in barly. J. Sci. Food. Agri, 35: 725-730.
Williams, R.D. and Hoagland, E. (1982). The effects of naturally Occurring phenolic compounds on seed germination.Weed Sci. 30: 206.
Yang, C.M., Chang, I.F., Lin, S.J. and C.H. (2004). Effects of three allelopathic phenolics on chlorophyll accumulation of rice (Oryza sativa) seedling: II. stimulation of consumption-Orientation. Bot. Bull. Acad. 45: 112-125.
Zeng, R. S., Luo, S. M., Shi, Y.H., Shi, M.B. and Tu, C.Y. (2001). Physiological and biochemical mechanism of allelopathy of Secalonic acid F of higher plants. Agronomy Journal. 93: 72-79.
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Appel, H. M. (1993). Phenolics in ecological interactions: The importance of oxidation. Chem. Ecol. 19: 1521-1552.
Bais, H.P., Vepechedu. R., Gilroy. S., Callaway. R.M. and vivanco. J.M. (2003) Allelopathy: From molecules and genes to species interactions. Science 301: 1377-1380.
Bruisma, j. (1963).The quantitative analysis of chlorophyll a & b in plant extract .Photochem. Photobiol, 12: 241.249.
Brunn, S.A., Muday, G.K. and Haworth, P. (1992). Auxin transport and the interaction of phytotropins. Plant Physiol. 98: 101-107.
Chance, B., and Maehley, A. (1955). Assay of catalases and peroxidase, Methods in Enzymology, 2, 764-775.
Ervin, G.N. and Wetzel, R.G. (2000). Allelochemical autotoxicity in the emergent wetland macrophyte Juncus effusus ( Juncaceae ) Am. J. Bot. 87: 853-860.
Fettell, N.A. and H. S. Gill 1995. "Long-term effects of tillage, stubble, and nitrogen management on properties of a red-brown earth." Australian Journal of Experimental Agriculture 35: 923-928.
Jimenez-orornio, J.J. and Gliessman, S.R. (1987). In allelochemicals role in agriculture and foresty. American Chemical Society, Washington, Dc. pp: 262-274.
Katalin, N. Omarov, R.T., Evdei, L. and Herman lips, S. (2000). Distribution of the Mo-enzymes. aldehyde oxidase, xanthine dehydrogenase and nitrate reductase in maize (Zea maize L.) roots as affected by nitrogen and Salinity. Plan Sci. 155: 45-58.
Khalid, Sh., Ahmad, T. and Shad, R.A. (2002). Use of allelopathy in agriculture. Asian Journal of Sciences. 3: 292-297.
Koroi, S.A.A. (1989). Gele electrophores tishe and spectrophoto metrscho unter uchungen zomeinfiuss der tem pelature auf straktur der amylase and peroxidose isoenzyme. Physiol.Veg, 20: 15-23.
Malinda, D. K. (1995). Factors in conservation farming that reduce erosion. Australian Journal of Experimental Agriculture 35: 969-978.
Matile, P. Hortensteiner, S., Thomas, H. and Krautler, B. (1996). Chlorophyllase in the chloroplast envelope. Planta. 201: 96-99.
Narwal, S.S and Tauro, P. (1996). Suggested methodology for allelopathy: field observations and Methodology. P. 255-260.
Purvis, C. E. (1990). Differential response of wheat to retained crop stubbles. I. Effect of stubble type and degree of decomposition". Australian Journal of Agricultural Research 41: 225-242.
PengS-L and Shao. H. (2001). Reaserch significance and foreground of allelopathy.Chin J Appl Ecol, 12:780-786.
Rice, E. L. (1984). Allelopathy. 2nd ed. Academic press, Orland. pp: 226-291
Rizivi, S. J. H. and Rizivi. V. (1992). Expoitation of allelochemiocals in improving crop productivity. Cand hall, London. p: 443-473
Shiming, L. (2003). Allelopathy in South china agroecosystems. Institute of Tropical and Subtropical Ecology. P: 40-54
Sym, G.L. (1984). Optimisation of the invivo assay conditions for nitrate reductase in barly. J. Sci. Food. Agri, 35: 725-730.
Williams, R.D. and Hoagland, E. (1982). The effects of naturally Occurring phenolic compounds on seed germination.Weed Sci. 30: 206.
Yang, C.M., Chang, I.F., Lin, S.J. and C.H. (2004). Effects of three allelopathic phenolics on chlorophyll accumulation of rice (Oryza sativa) seedling: II. stimulation of consumption-Orientation. Bot. Bull. Acad. 45: 112-125.
Zeng, R. S., Luo, S. M., Shi, Y.H., Shi, M.B. and Tu, C.Y. (2001). Physiological and biochemical mechanism of allelopathy of Secalonic acid F of higher plants. Agronomy Journal. 93: 72-79.