بررسی نوع ریزنمونه بذری و منبع کربن در بهینهسازی تولید پینههای مناسب فستوکای بلند در شرایط درون شیشهای
الموضوعات :متین دولتی 1 , مصطفی خوشحال سرمست 2 , سید جواد موسوی زاده 3
1 - گروه علوم باغبانی و فضای سبز، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.
2 - گروه علوم باغبانی و فضای سبز، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.
3 - گروه علوم باغبانی و فضای سبز، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.
الکلمات المفتاحية: جنین, چمن, فستوکای بلند, ریز نمونه, پینه, مالتوز,
ملخص المقالة :
صنعت چمن در برخی از کشورها درآمد چندین میلیار دلاری در سال را فراهم میکند. فستوکای بلند Festuca arundinacea Schreb.)) یک چمن پوششی فصل خنک چندساله است که دارای گردهافشانی آزاد میباشد. ریزافزایی و انتقال ژن به گونههای تک لپه مانند فستوکای بلند همواره با راندمان پایینی همراه بوده است. بنابراین بررسی اثر نوع ریز نمونه بذری و منبع کربن به کار رفته در محیط کشت میتواند از طریق بهینه سازی تولید پینه مناسب، برای انتقال ژن به این گیاه سودمند باشد. پس از گندزدایی سطحی بذر فستوکای بلند با الکل و کلراکس، انواع ریز نمونههای بذری دارای جنین و فاقد جنین برای ارزیابی قدرت تولید پینه در محیط MS کشت شدند. شکر معمولی، مالتوز و نسبت برابری از مالتوز و شکر معمولی نیز به عنوان منبع کربن مورد بررسی قرار گرفتند. در پایان، پینههای جنینزای القاء شده روی محیط دارای 10 میلیگرم در لیتر 2,4-D و 05/0 میلیگرم در لیتر BA مورد باززایی قرار گرفتند. نتایج بررسی نوع ریز نمونه بذری به طور آشکار حاکی از ضرورت وجود جنین در ریز نمونه برای القاء پینه دارد. ریز نمونههای فاقد جنین که به صورت طولی و عرضی برش خورده بودند قادر به القاء پینه نبودند. بررسی سه منبع کربن متفاوت نشان میدهد که مالتوز و شکر معمولی به طور جداگانه میزان کلروفیل کل و کارتنوئید را در برگ گیاهان باززایی شده بهبود میبخشند در حالی که استفاده همزمان از شکر معمولی و مالتوز چنین اثری را نشان نداد. برش عرضی به همراه جنین در بذر فستوکای بلند و استفاده از مالتوز به ترتیب بهترین ریز نمونه و منبع کربن برای القاء تولید پینه و پرآوری شاخساره در این گیاه است. محیط دارای مالتوز پینههای سفتتر و زرد رنگ بیشتری در مقایسه با محیطهای دیگر تولید میکند که ریز نمونهای مناسب برای انتقال ژن به فستوکای بلند میباشد.
Ahmad, T., Akhtar Abbasi, N., Ahmad Hafiz, I. and Ansar, A. (2007). Comparison of sucrose and sorbitol as main carbon energy source in morphogenesis of peach rootstock GF-677. Pakestanian. Journal Botany, 39(4):1264–1275.
Bai, Y. and Qu, R. (2001). Factors influencing tissue culture responses of mature seeds and immature embryos in turf-type tall fescue. Plant Breed, 120: 239-242
Batty, N. and Dunwell J. (1989). Effect of maltose on the response of potato anthers in culture. Plant Cell, Tissue and Organ Culture, 18:221–226
Blanc, G., Michaux-Ferrière, N., Teisson, C. Lardet, T. and Carron, M.P. (1999). Effects of carbohydrate addition on the induction of somatic embryogenesis in Hevea brasiliensis. Plant Cell, Tissue Organ Cult, 59(2):103–112
Buah, J., Tachie-Menson, J.W., Addae, G. and Asare, P. (2011). Sugarcane juice as an alternative carbon source for in vitro culture of plantains and bananas. American Journal of Food Technology, 6(8):685–694
Cross, J.W., Bonos, S.A., Huang, B. and Meyer, W.A. (2013). Evaluation of heat and drought as components of summer stress on tall fescue genotypes. Hort Science 48:1562–1567.
Dong S. and Qu R. (2005). High efficiency transformation of tall fescue with Agrobacterium tumefaciens. Plant Science, 168: 1453–1458.
Gao, C., Long, D., Lenk, I. and Nielsen, K.K. (2008). Comparative analysis of transgenic tall fescue (Festuca arundinacea Schreb.) plants obtained by Agrobacterium-mediated transformation and particle bombardment. Plant Cell Reports, 27: 1601–1609.
George, E.F., Hall, M.A. and Klerk, G.J.D. (2008). The components of plant tissue culture media I: macro-and micro-nutrients. Plant Propagation by Tissue Culture, 5: 65–113
Ha, S.B., Wu, F.S., Thorne, T.K. (1992). Transgenic turf-type tall fescue (Festuca arundinacea Schreb.) plants regenerated from protoplasts. Plant Cell Reports, 11: 601– 604.
Hartmann, H.T., Kester, D.E., Davies, F.T. and Geneve, R.L. (2002). Plant propagation, principles and practices (p. 880). Upper Saddle River, NJ: Pearson Education, Inc.
Hiscox, J.D. and Israelstam, G.F. (1979). A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany, 57: 1332-1334.
Hosseini, H.R., Salehi, H. and Alichi, M. (2019). Acquirement of CRY8DB Transgenic Tall Fescue (Festuca arundinacea Schreb.) by Agrobacterium tumefaciens to Develop Resistance Against Pentodon idiota Herbest. Molecular Biotechnology, 61:528-540.
Hu, Y., Jia, W., Wang, J., Zhang, Y., Yang, L. and Lin, Z. (2005). Transgenic tall fescue containing the Agrobacterium tumefaciens ipt gene shows enhanced cold tolerance. Plant Cell Reports, 23:705–709
Hu, T., Wang, T., Wang, G., Bi, A., Wassie, M., Xie, Y., Xu, H. and Chen, L. (2021). Overexpression of FaHSP17.8-CII improves cadmium accumulation and tolerance in tall fescue shoots by promoting chloroplast stability and photosynthetic electron transfer of PSII. Journal of Hazardous Materials, 417:125932
Lee, K-W., Choi, G., Kim, K., Ji, H., Park, H., Seo, S., Kim, M.J. and Lee, S-H. (2012). Comparison of callus induction and plant regeneration from twenty tall fescue varieties. African Journal of Biotechnology, 11: 3553-3557
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiology Plant, 15:473–497
Morris, P., Dalton, S., Langdon, T. Hauck, B. and de Buanafina, M.M.O. (2017). Expression of a fungal ferulic acid esterase in suspension cultures of tall fescue (Festuca arundinacea) decreases cell wall feruloylation and increases rates of cell wall digestion. Plant Cell Tissue and Organ Culture, 129:181–193.
Patel, M., Dewey, R.E. and Qu, R. (2013). Enhancing Agrobacterium tumefaciens-mediated transformation efficiency of perennial ryegrass and rice using heat and high maltose treatments during bacterial infection. Plant Cell Tissue and Organ Culture, 114, 19–29.
Rahman, M.H. and Islam, M. (2010). Role of sucrose, glucose and maltose on conventional potato micropropagation. Journal Agriculture Technology, 6(4): 733-739
Ruzic´, Lazic, T. and Cerovic, R. (2008). Micropropagation of some Prunus and Pyrus genotypes in vitro as affected by different carbon sources. Acta Hortic, 795:413–418
Sarmast, M.K., Salehi, H. and Zarei, M. (2018). A preliminary experiment on Agrobacterium tumefaciens-mediated transformation of the P5CS1 gene in tall fescue. Journal of Ornamental Plants, 8:79:86
Sarmast, M.K., Salehi, H. and Niazi A. (2015). Biochemical differences underlie varying drought tolerance in four Festuca arundinacea Schreb. genotypes subjected to short water scarcity. Acta Physiology Plant. 37:192
Thomson M. and Thorpe T. (1987). Metabolic and non metabolic roles of carbohydrates. In: Bong JM, Durzan DJ (eds.) Cell and tissue culture in forestry. Martinus Nijhoff Publisher, Dardrecht,
Turgeon, AL. (2008). Turfgrass Management. 8th ed. Prentice-Hall, Englewood. NJ.
Wang Z.Y. and Gee Y. (2004). Agrobacterium-mediated high efficiency transformation of tall fescue (Festuca arundinacea). Journal Plant Physiology, 162: 103-113.
Wu, Y., Chen, Q., Chen, M., Chen, J. and Wang, X. (2005). Salt-tolerant transgenic perennial ryegrass (Lolium perenne L.). obtained by Agrobacterium tumefaciens-mediated transformation of the vacuolar Na+/H+ antiporter gene. Plant Science, 169: 65–73.
Yaseen, M., Ahmad T., Sablok, G., Standardi, A. and Ahmad Hafiz, I. (2013). Review: role of carbon sources for in vitro plant growth and development. Molecular Biology, 40: 2837–2849.
Zhou, B., Luo, H. and Qu, R. (2016). Expression of the shrimp antimicrobial peptide penaeidin 4-1 confers resistance against brown patch disease in tall fescue. Plant Cell Tissue and Organ Culture, 125:599–603.
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Ahmad, T., Akhtar Abbasi, N., Ahmad Hafiz, I. and Ansar, A. (2007). Comparison of sucrose and sorbitol as main carbon energy source in morphogenesis of peach rootstock GF-677. Pakestanian. Journal Botany, 39(4):1264–1275.
Bai, Y. and Qu, R. (2001). Factors influencing tissue culture responses of mature seeds and immature embryos in turf-type tall fescue. Plant Breed, 120: 239-242
Batty, N. and Dunwell J. (1989). Effect of maltose on the response of potato anthers in culture. Plant Cell, Tissue and Organ Culture, 18:221–226
Blanc, G., Michaux-Ferrière, N., Teisson, C. Lardet, T. and Carron, M.P. (1999). Effects of carbohydrate addition on the induction of somatic embryogenesis in Hevea brasiliensis. Plant Cell, Tissue Organ Cult, 59(2):103–112
Buah, J., Tachie-Menson, J.W., Addae, G. and Asare, P. (2011). Sugarcane juice as an alternative carbon source for in vitro culture of plantains and bananas. American Journal of Food Technology, 6(8):685–694
Cross, J.W., Bonos, S.A., Huang, B. and Meyer, W.A. (2013). Evaluation of heat and drought as components of summer stress on tall fescue genotypes. Hort Science 48:1562–1567.
Dong S. and Qu R. (2005). High efficiency transformation of tall fescue with Agrobacterium tumefaciens. Plant Science, 168: 1453–1458.
Gao, C., Long, D., Lenk, I. and Nielsen, K.K. (2008). Comparative analysis of transgenic tall fescue (Festuca arundinacea Schreb.) plants obtained by Agrobacterium-mediated transformation and particle bombardment. Plant Cell Reports, 27: 1601–1609.
George, E.F., Hall, M.A. and Klerk, G.J.D. (2008). The components of plant tissue culture media I: macro-and micro-nutrients. Plant Propagation by Tissue Culture, 5: 65–113
Ha, S.B., Wu, F.S., Thorne, T.K. (1992). Transgenic turf-type tall fescue (Festuca arundinacea Schreb.) plants regenerated from protoplasts. Plant Cell Reports, 11: 601– 604.
Hartmann, H.T., Kester, D.E., Davies, F.T. and Geneve, R.L. (2002). Plant propagation, principles and practices (p. 880). Upper Saddle River, NJ: Pearson Education, Inc.
Hiscox, J.D. and Israelstam, G.F. (1979). A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany, 57: 1332-1334.
Hosseini, H.R., Salehi, H. and Alichi, M. (2019). Acquirement of CRY8DB Transgenic Tall Fescue (Festuca arundinacea Schreb.) by Agrobacterium tumefaciens to Develop Resistance Against Pentodon idiota Herbest. Molecular Biotechnology, 61:528-540.
Hu, Y., Jia, W., Wang, J., Zhang, Y., Yang, L. and Lin, Z. (2005). Transgenic tall fescue containing the Agrobacterium tumefaciens ipt gene shows enhanced cold tolerance. Plant Cell Reports, 23:705–709
Hu, T., Wang, T., Wang, G., Bi, A., Wassie, M., Xie, Y., Xu, H. and Chen, L. (2021). Overexpression of FaHSP17.8-CII improves cadmium accumulation and tolerance in tall fescue shoots by promoting chloroplast stability and photosynthetic electron transfer of PSII. Journal of Hazardous Materials, 417:125932
Lee, K-W., Choi, G., Kim, K., Ji, H., Park, H., Seo, S., Kim, M.J. and Lee, S-H. (2012). Comparison of callus induction and plant regeneration from twenty tall fescue varieties. African Journal of Biotechnology, 11: 3553-3557
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiology Plant, 15:473–497
Morris, P., Dalton, S., Langdon, T. Hauck, B. and de Buanafina, M.M.O. (2017). Expression of a fungal ferulic acid esterase in suspension cultures of tall fescue (Festuca arundinacea) decreases cell wall feruloylation and increases rates of cell wall digestion. Plant Cell Tissue and Organ Culture, 129:181–193.
Patel, M., Dewey, R.E. and Qu, R. (2013). Enhancing Agrobacterium tumefaciens-mediated transformation efficiency of perennial ryegrass and rice using heat and high maltose treatments during bacterial infection. Plant Cell Tissue and Organ Culture, 114, 19–29.
Rahman, M.H. and Islam, M. (2010). Role of sucrose, glucose and maltose on conventional potato micropropagation. Journal Agriculture Technology, 6(4): 733-739
Ruzic´, Lazic, T. and Cerovic, R. (2008). Micropropagation of some Prunus and Pyrus genotypes in vitro as affected by different carbon sources. Acta Hortic, 795:413–418
Sarmast, M.K., Salehi, H. and Zarei, M. (2018). A preliminary experiment on Agrobacterium tumefaciens-mediated transformation of the P5CS1 gene in tall fescue. Journal of Ornamental Plants, 8:79:86
Sarmast, M.K., Salehi, H. and Niazi A. (2015). Biochemical differences underlie varying drought tolerance in four Festuca arundinacea Schreb. genotypes subjected to short water scarcity. Acta Physiology Plant. 37:192
Thomson M. and Thorpe T. (1987). Metabolic and non metabolic roles of carbohydrates. In: Bong JM, Durzan DJ (eds.) Cell and tissue culture in forestry. Martinus Nijhoff Publisher, Dardrecht,
Turgeon, AL. (2008). Turfgrass Management. 8th ed. Prentice-Hall, Englewood. NJ.
Wang Z.Y. and Gee Y. (2004). Agrobacterium-mediated high efficiency transformation of tall fescue (Festuca arundinacea). Journal Plant Physiology, 162: 103-113.
Wu, Y., Chen, Q., Chen, M., Chen, J. and Wang, X. (2005). Salt-tolerant transgenic perennial ryegrass (Lolium perenne L.). obtained by Agrobacterium tumefaciens-mediated transformation of the vacuolar Na+/H+ antiporter gene. Plant Science, 169: 65–73.
Yaseen, M., Ahmad T., Sablok, G., Standardi, A. and Ahmad Hafiz, I. (2013). Review: role of carbon sources for in vitro plant growth and development. Molecular Biology, 40: 2837–2849.
Zhou, B., Luo, H. and Qu, R. (2016). Expression of the shrimp antimicrobial peptide penaeidin 4-1 confers resistance against brown patch disease in tall fescue. Plant Cell Tissue and Organ Culture, 125:599–603.