تأثیر پرایمینگ بذر با اسید سالیسیلیک بر شاخصهای جوانه زنی و رشد گیاه گل گاوزبان تحت تنش شوری
الموضوعات :ابوالفضل لولایی 1 , علی خلیلی 2 , هدایت محمدی 3 , سارا اسلامی 4
1 - اداره کل منابع طبیعی و آبخیزداری استان تهران
2 - کارشناس ارشد اکولوژی مرتع، دفتر امور مراتع و گیاهان دارویی سازمان جنگلها، مراتع و ابخیزداری کشور تهران، ایران
3 - کارشناس باغبانی
4 - کارشناس ارشد زراعت، اداره کل تعاون روستایی استان هرمزگان
الکلمات المفتاحية: شوری, اسیدسالیسیلیک, گل گاوزبان, درصد جوانه زنی بذر, غلظت پروتئین بذر,
ملخص المقالة :
یکی از روشهای موثر برای غلبه بر تنش در جوانه زنی بذور، استفاده از تکنیک پرایمینگ بذر است. به این منظور به بررسی تأثیر پیش تیمار اسیدسالسیلیک بر جوانه زنی و برخی خصوصیات بذر گیاه دارویی گل گاوزبان (Borago officinalis. L ، در شرایط تنش شوری، آزمایشی به صورت فاکتوریل در قالب طرح کامل تصادفی با سه سطح اسیدسالسیلیک با غلظت غلظت (500، 1000 و 1500 میلی مولار) و تنش شوری با غلظت های (50، 100 و 150 میلی مولار) بودند. صفات مورد آزمایش شامل طول، وزن و نسبت ریشه چه و ساقه چه، غلظت پروتئین بذر، درصد جوانه زنی بذر بودند. بر اساس نتایج بدست آمده در پاسخ به شرایط تنش شـوری، سطح رشد مرفولوژیکی بذور به شدت تغییر کرد، کاهش طول ریشه چه و ساقه چه و همینطور وزن آنها کاهش یافت. ولی تیمـار با تغییر این نسبت، باعث حفظ و ثبـات فعالیت مرفولوژیکی در بذور گل گاوزبان شد. بر اساس نتایج به دست آمده، تیمار سالیسیلیک اسید تحت شرایط تـنش شـوری میتواند برای تقویت فعالیت جوانه زنی و رشدی گل گاوزبان میشود.
Amiri, S., Sabrian, V., Abbasi, A., Enayati, V. and Janmohammadi, M. 2009. Effect of salinity stress on germination and early growth of Alyssum bracteatum, National Conference of Medicinal Plants. Rice and Citrus Research Institute, Sari University of Agricultural Sciences and Natural Resources.
Amirjani, M.R. 2010. Effects of salinity stress on growth, mineral composition, proline content, antioxidant enzymes of soybean. American Journal of Physiology 5(6): 350-360.
Arfan, M., Athar, H.R. and Ashraf, M. 2007. Does exogenous application of salicylic acid through the rooting medium modulate growth and photosynthetic capacity in two differently adapted spring wheat cultivars under salt stress. J. Plant Physiol. 164: 685-694
Ashraf, M. and Orooj, A. 2006. Salt stress effects on growth, ion accumulation and seed oil concentration in an arid zone traditional medicinal plant ajwain (Trachyspermum ammi [L.] Sprague). Journal of Arid Environments, 64: 209–220.
Azooz, M.M. 2009. Salt stress mitigation by seed priming with salicylic acid in two faba bean genotypes differing in salt tolerance. Int. J. Agric. Biol. 11(4), 343-350.
Baalbaki, R,Z., Zurayk, R.A., Blelk, M.M., and Tahouk, S.N. 1999. Germination and seedling development of drought tolerant and susceptible wheat under moisture stress. Seed. Sci. Technol. 27: 291-302.
Bandeoglu, E., Eyidogan, F., Yucel, M. and Oktem, H. 2004. Antioxidant responses of shoots and roots of lentil to NaCl-salinity stress. Plant Growth Regulation 42: 69-77.
Bannayan, M., Nadjafi, F., Azizi, M., Tabrizi, L. and Rastgoo, M. 2008. Yield and seed quality of Plantago ovata and Nigella sativa under different irrigation treatments. Industrial Crops and Products, 27:11–16. (Journal)
Borsani, O., Valpuseta, V., and Botella, M.A. 2001. Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings. J. Plant Physiol. 126, 1024-1030.
Bradford, M. 1976. A rapid and sensitive method for the quantitation of protein utilizing the principle of protein-dye binding. Annu. Rev. Biochim. 72: 248-254.
Broumandrezazadeh, Z., and Kochaki, A. 2014. Investigating the germination response of fenugreek, fennel, and dill seeds to osmotic and matric potentials caused by sodium chloride and polyethylene glycol 6000 at different temperatures, Iranian Agricultural Research Journal. 3: 217-207.
Cavalcanti, F., Lima, J.P., Silva, S., Viegas, R. and Silveria, J. 2007. Roots and leaves display contrasting oxidative response during salt stress and recovery in cowpea. Journal of Plant Physiology 164: 591-600.
Chinnusamy, V., Jagendorf, A. and Zhu, J.K. 2005. Understanding and improving salt tolerance in plants. Crop Sciences. 45: 437-448.
Craine, J.M. 2005.Reconciling plant strategy theories of Grime and Tilman. Journal of Ecology 93: 1041-1052.
Dolat Abadian, A., Seyed Ali M. and Etemadi, F. 2017. The effect of salicylic acid pretreatment on wheat seed germination under salt stress conditions. Iran biology magazine. 21(4).
Dong, Y., Ji, T. and Dong, S. 2007. Stress responses to rapid temperature changes of the juvenile sea cucumber (Apostichopus japonicus Selenka). Journal of Ocean University of Chin 6: 275-280.
Droodi, A., and Ghasemi, M., and Sohani, A. 2016. The effect of salicylic acid on some characteristics of borage flowers under drought stress conditions. Proceedings of the 7th National Conference on Sustainable Agriculture and Natural Resources.
El-Tayeb, M.A. 2005. Response of barley Grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation, 45:215-225.
Fallah, A., Farahmand, A. and Moradi, F. 2014. The effect of salinity stress in different stages of growth on some morphophysiological traits of two rice cultivars under greenhouse conditions. Journal of Agriculture (Research and Production), 107: 182-175.
Farooq, M., Basra, S.M.A., Tabassum, R. and Afzal, I. 2006. Enhancing the performance of direct seeded fine rice by seed priming. Plant Production Science 9: 446-456.
Gholinejad, R., Siros Mehr, A., and Fakheri, B.A. 2015. Evaluation of irrigation regimen and organic fertilizers on quantitative and qualitative yield of borage, Ecophysiological journal of agricultural plants, 10(3): 683-696.
Hanan, E.D. 2007. Influence of salicylic acid on stress tolerance during seed germination of Triticum aestivum and Hordeum vulgare. Biol. Res. 1: 40- 48..
Hashemi, S.A., Imam, Y. and Pirste Anoushe, H. 2014. The effect of time and method of application of salicylic acid on the growth process, yield and yield components of barley under salinity stress conditions. Crop Physiology, 6 (24): 5-18.
Hero, A. 1978. Colored flora of Iran, first volume, publications of the National Association for the Protection of Natural Resources and Human Environment, number 74.
Horvath, E., Szalai, G. and Janda, T., 2007. Introduction of abiotic stress tolerance by salicylic acid signaling. J. Plant Growth Regul. 26, 290-300.
Idris, H., and Marashi, S.K. 2017. Investigating the effect of different methods of salicylic acid application on reducing the effects of salinity stress in wheat lands without drainage system. Scientific Research Quarterly Journal of Plant Physiology - Islamic Azad University, Ahvaz Branch, 10(39): 131-146.
Jabbarzadeh, Z., Khosh-Kkhui, M., and Salehi, H. 2009. The effect of foliar applied salicylic acid on flowering of African violet. Aust. J. Bas. Appl. Sci. 3(4): 4693-4696.
Kabiri, R. and Naghizadeh, M. 2014. Investigating the effect of salicylic acid pretreatment on germination and early growth of black seed seedlings under salt stress conditions. Iranian Journal of Seed Science and Technology, Hald IV, No. 1, page 61-72.
Kaur, S., Gupta, A.K. and Kaur, N. 2006. Effect of hydro-and osmo priming of chickpea (Cicer orietinum L.) seeds on enzymes of sucrose and nitrogen metabolism in nodules. Plant Growth Regulation 49: 177-182.
Kaydan, D., and Yagmur, M. 2008. Germination, seedling growth and relative water content of shoot in different seed sizes of triticale under osmotic stress of water and NaCl. Afr. J. Biotechnol. 7: 2862-2868
Kaydan, D., Yagmur, M. and Okut, N. 2007. Effects of salicylic acid on the growth and some physiological characters in salt stressed wheat (Triticum aestivum L.). J. Tarim Bilimleri Dergisi 13: 114-119.
Khodary, S.E.A. 2004. Effect of salicylic acid on the growth, photosynthesis and carbohydrate
metabolism in salt-stressed maize plants. Int. J. Agri. Biol. 6: 5–8.
Khodary, S.E.A. 2004. Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt stressed maize plants. Int. J. Agric. Biol. 6(1): 5-8.
Khomeri, A., Sarani, S.A. and Dehmardeh, M. 2016. Investigating the effect of salinity on seed germination and seedling growth in six species of medicinal plants. Iranian Medicinal and Aromatic Plants Research Journal. Volume 3, Number 3, Serial 37. Page 331-339.
Khorsandi, A., Hosni, A., Sefidkan, F., Shiraz, H. and Khorsand, A. 2019. The effect of salinity on the yield and amount of essential oil and medicinal compounds of Mexican flower. Journal of Production and Processing of Medicinal Plants 3(26):438-451.
Lolaei, A., Khalili, A., and Moshefifar, Sh. 2018. Investigating some medicinal properties of borage flower in the treatment of salicylic acid and biological fertilizers. Journal of Agricultural Research.
Madady, M. Khomari, S., Javadi, A. and Sofalian, A. 2016. The effect of priming with calcium nitrate and zinc oxide on seed germination and seedling growth of corncockle under salinity stress, Journal of Plant Process and Function, 5(15): 169-179. (In Persian)(Journal)
Magsoumi Holasu, S. and Porakbar, L. 2013. To investigate the effects of salinity stress on the growth and some physiological indicators of the grain of wheat plants.
Mahmoudirad, Z. and Noorafken, Z. 2018. The effect of seed pretreatment with salicylic acid on the germination of Maritigal under salt stress. Journal of Seed Research, 9(4): 11-21.
Manchanda, G. and Garg, N. 2008. Salinity and its effects on the functional biology of legumes. Acta Physiology Plant, 30: 595-618. (Journal)
Mane, A.V., Deshpande, T.V., Wagh, V.B., Karadge, B.A., and Samant, J.S. 2011. Acritical review on physiological changes associated with reference to salinity. International Journal of Environmental Science 1(6): 1192-1216.
Mengel, K. and Kirkby, W. 2004. Principles of Plant Nutrition. Kluwer, The Netherlands. (Book)
Mirzaei, M.M., Ghorbani, S., Rozbahani, A. and Qadri, A. 2016. To investigate the use of biological fertilizers on quantitative and qualitative traits of borage flower in water shortage conditions. Journal of Agricultural Research in the Margin of the Desert, 13(2): 157-173.
Munnas, R. and Tester, M. 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology 59: 651-681.
Nichols, P.G.H., Malik, A.I., Stockdale, M. and Colmer, T.D. 2009. Salt tolerance and avoidance mechanisms at germination of annual pasture legumes: importance for adaptation to saline environments. Plant and Soil, 315:241–255. (Journal)
Oscar, V., Boscaiu, M., Angel, M. and Estrelles, E. 2004. Responses to salt stress in the halophyte Plantago crassifolia (Plantaginaceae). Journal of Arid Environments, 58:463–481. (Journal)
Poshtdar, A., Abdali Mashhadi, A. and Monjezi, F. 2015. Response germination and vigor
index seed of milk thistle to priming with salicylic acid under salt stress. The second
conference on New findings in the Environment and Agricultural Ecosystems. Institute New
Energys and Environment of Tehran University
Ragami, M., Staji, A., Bagheri, V. and Ariakia, A. 2015. Effect of salinity stress and salicylic acid on some morphophysiological characteristics of eggplant (Solanum melongena var. Taki) in soilless cultivation system. Quarterly Journal of Science and Techniques of Greenhouse Cultivation. 7(27): 77-87.
Rahimi, A.R., Mashayekhi, K., Hemmati, Kh., and Dordipour, E. 2009. Effect of salicylic acid and mineral nutrition on fruit yield and yield components of Coriander (Coriandrum sativum L.). J. Plan. Prod. 16: 4. 149-156. (In Persian)
Rajasekaran, L.R., Stiles, A., Surette, M.A., Sturz, A.V, Blake, T.J., Caldwell, C. and Nowak, J. 2002. Stand Establishment Technologies for Processing Carrots: Effects of various temperature regimes on germination and role of salicylates in promoting germination at low temperatures. Can. J. Plant Sci. 82: 443-450.
Rezvani Moghada, P., Lashkari, A. and Amin Ghafouri, A. 2014. Estimation of cardinal temperatours of (Echium amoniom). With application of regression models. Iranian Journal of Field Crops research, 12(2):164-169.
Rostami, M. and Mazaheri, Sh. 2009. Investigating the effects of salinity stress on the germination of carnation seeds (Dianthus barbatus). National Conference of Medicinal Plants.
Rubinigg, M., Posthumus, F., Ferschke, M., Theo, J. and Stulen, I. 2003. Effects of NaCl salinity on 15N-nitrate fluxes and specific root length in the halophyte Plantago maritima L. Plant and Soil, 250: 201–213. (Journal)
Safari, H., Madah, Sh., Azari, Auman and Heshmati, M. 2012. To investigate the effect of salicylic acid pretreatment on sesame seed germination under salt stress conditions. The first national conference on salinity stress in plants and agricultural development, Shahid Madani University of Azerbaijan, p 883-888.
Sepehri, A., Abbasi, R. and Kerami, A. 2014. Effect of drought stress and salicylic acid on yield and yield components of red bean genotypes. Journal of Agriculture, 17(2): 503-516.
Shaheen A.M., Rizk F.A., Abdel-Aal F.S., and Habib H.A.M. 2011. Production of safe and economic onion bulbs. Int. J. Acad. Res., 3(1): 527-532.
Shakirova F.M., and Sahabutdinova D.R. 2003. Changes in the hormonal status of wheat seedlings induced by salicylic aicd and salinity. Plant Science 164: 317-322.
Sharma, A.K. 2002. Bio fertilizers for Sustainable Agriculture. Agrobios, India 407p.
Shekhari, F. and Baljani, R., Saba, J., Shekari, F. and Zangani, A. 2019. To investigate the effect of salicylic acid on the photosynthetic activities of flower yield in borage plant using seed priming method. Journal of Medicinal Plants. P 34-42.
Singh, B. and Usha K. 2003. Salicylic acid induced physiological and biochemical changes
in wheat seedlings under water stress. Plant Growth Regul. 39: 137–141.
Singh, B. and Usha K. 2003. Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regul. 39: 137–141.
Taherian, N., Naghdibadi H.A., MehrAfarin A. and Nikkhah Basti, A. 2019. In the research of researchers regarding the study of the effect of salinity stress on the germination of the medicinal plant Indian Senna. National Conference of Medicinal Plants.
Wang, L., Chen, S., Kong W., Li, S., and Archbold, D.D. 2006. Salicylic acid pretreatment alleviates chilling injury and affects the antioxidant system and heat shock proteins of peaches during cold storage. Postharvest Biology and Technology 41: 244-251.
Yadgari, G. 2014. Investigating the amount and speed of seed germination of twelve medicinal plants under salt stress. Iranian Seed Science and Research Quarterly. Second year / number one / 2014 (27-36).
Yonis, M.E., Abbas, M.A. and Shukry, W.M. 1993. Effect of salinity of growth and metabolism of Phaseolus vulgaris. Biologia Plantarum. 35(3): 417- 424.
Yoon, B.Y.H., Lang, H.J. and Greg Cobb, B. 1997. Priming with salt solutions improves germination of pansy seed at high temperatures. Hort. Sci. 32: 248-50.
Zarei, B., Fazeli, A. and Taghipour, Z. 2019. Investigating the effect of salicylic acid on germination and the activity of some black seed antioxidant enzymes under salt stress conditions, Seed Science and Research District, 7th year, 2nd issue, p: 241-253.
Zhu, JK. 2001. Plant salt tolerance. Trends in Plant Science. 6: 66–71.
Zhu, JK. 2007. Plant Salt Stress. John Wiley & Sons, Ltd.
